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Study on biochemical and morphological changes in the liver and metabolism of the body and liver during isolated liver perfusion in weanling swine.

断奶仔猪离体肝灌注过程中肝脏生化、形态变化及机体和肝脏代谢的研究

基本信息

批准号：
08672055
负责人：
SEO Takahiko
金额：
$ 1.22万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

The present study was designed to investigate the biochemical and morphologic changes in the liver, and also metabolism during isolated liver perfusion with double bypass using automatic blood pumps. Isolated liver perfusion was established with bypass between the hepatic and portal veins in 15 mongrel dogs weighing 3.5 to 6.0 kg and in 18 weanling Yorkshire swine weighing 6.8 to 12.2 kg, at a flow rate of 10,20,30 ml/min/kg for up to 4 hours. Venous blood from the intestine and lower body was bypassed to the superior vena cava with another extracorporeal circuit. Perfusate glutamic pyruvic transaminase and lactate concentrations did not change during liver perfusion in all groups in swine, in contrast to ischemic changes at 10 ml/min/kg and congestion at 30 ml/min/kg in the liver of the puppy. Energy charge was maintained. Microscopically, normal histology of the hepatic parenchyma and portal tract structures was preserved in three groups. Transmission electron microscopy showed no gross structural abnormalities in most of the hepatocytes for up to 2 hours in three groups. However, swelling of the mitochondria and smooth endoplasmic reticulum was visualized occasionally in a very small number of the hepatocytes after more than 3 hours of perfusion. This phenomenon resembled that at the early phase of shock. This swelling was observed in more hepatocytes at a flow rate of 10 ml/min/kg. Glycogen granules decreased with time in some animals. On metabolic analysis, blood glucose gradually increased in the body at the anhepatic phase. In the liver, blood glucose rapidly increased at the commencement of perfusion and gradually decreased with time. Total cholesterol and total protein gradually decreased with time in the body, and rapidly decreased at the commencement and maintained in the liver. Those results demonstrated that the isolated liver perfusion strongly affected hepatic and general metabolism.

本研究旨在探讨自动血泵双旁路离体肝灌注过程中肝脏生化、形态学及代谢的变化。在15只体重为3.5 - 6.0 kg的杂种狗和18只体重为6.8 - 12.2 kg的断奶约克郡猪中，分别以10、20、30 ml/min/kg的流速进行肝静脉和门静脉间的旁路灌注，持续4小时。从肠道和下体的静脉血通过另一个体外回路分流到上腔静脉。与幼犬肝脏缺血（10 ml/min/kg）和充血（30 ml/min/kg）相比，各组猪肝脏灌注时谷丙转氨酶和乳酸浓度没有变化。能量电荷保持不变。镜下观察，三组肝脏实质及门静脉结构均保持正常组织学。透射电镜显示，三组肝细胞在2小时内大部分未见明显结构异常。然而，在灌注超过3小时后，极少数肝细胞可见线粒体肿胀和光滑的内质网。这种现象类似于休克初期。当流速为10 ml/min/kg时，在更多肝细胞中观察到这种肿胀。一些动物的糖原颗粒随着时间的推移而减少。代谢分析显示，在无肝期，体内血糖逐渐升高。肝脏中，血糖在灌注开始时迅速升高，随着时间的推移逐渐降低。总胆固醇和总蛋白在体内随着时间的推移逐渐降低，在开始时迅速下降并维持在肝脏中。这些结果表明，离体肝脏灌注强烈影响肝脏和全身代谢。