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Study on metabolism and microcirculation of the liver during isolated liver perfusion in weanling swine.

断奶猪离体肝灌注过程中肝脏代谢及微循环的研究

基本信息

批准号：
10671667
负责人：
SEO Takahiko
金额：
$ 2.11万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671667/
关键词：
artificial organs organ perfusion liver perfusion extracorporeal circuit organ preservation automatic blood pump metabolism children 臓器還流肝灌流対外循環回路

项目摘要

The present study was designed to investigate the metabolic changes in the body and the liver during isolated liver perfusion with double bypass using automatic blood pumps. Isolated liver perfusion was established with bypass between the hepatic vein and portal vein in 12 puppies weighing 3.7 to 6.5 kg and in 9 weanling Yorkshire swine weighing 7.4 to 12.7 kg, at a flow rate of 10 to 30 ml/min/kg for up to 4 hours. In the body, marked hypoglycemia was developed in the group with infusion without containing glucose and marked hyperglycemia in the group infused with 5% glucose solution. Blood glucose was stable at a level of 500 mg/dl in the group infused with 1.2% glucose solution. In the liver, blood glucose level was gradually increased in the group with perfusion at a 30-ml/min/kg flow rate, but blood glucose level was decreased in groups with 10-and 20-ml/min/kg perfusion. Total protein, albumin, and total cholesterol were gradually decreased with time in the body. They were also decreased in the liver, especially in the group perfused at a flow rate of 30 ml/min/kg.Then swine were divided in 4 groups ; group 1, liver perfusion with perfusate without glucose, group 2, with perfusate with 2.5% glucose solution, group 3 with perfusate not containing glucose but containing insulin, groups 4, with perfused containing 2.5% glucose and insulin. As a results, total protein and albumin were gradually decreased with time. The degree of decrease was mild in groups perfused with glucose-containing perfusate. Blood glucose was gradually increased in groups perfused with perfusate containing glucose. Insulin did not affect the glucose level. Total cholesterol was gradually decreased with time, however, triglyceride was kept constant or rather increased during perfusion. In all experiments, insulin did never influence the hepatic metabolism during isolated perfusion.

本研究旨在探讨在使用自动血泵的双转流隔离肝脏灌注过程中身体和肝脏的代谢变化。在12只体重为3.7 - 6.5 kg的幼犬和9只体重为7.4 - 12.7 kg的断奶约克郡猪中，通过肝静脉和门静脉之间的旁路建立了隔离肝脏灌注，流速为10 - 30 ml/min/kg，持续4小时。在体内，输注不含葡萄糖的组出现明显的低血糖，输注5%葡萄糖溶液的组出现明显的高血糖。输注1.2%葡萄糖溶液组的血糖稳定在500 mg/dl水平。在肝脏中，以30 ml/min/kg流速灌注的组中血糖水平逐渐升高，但以10 ml/min/kg和20 ml/min/kg灌注的组中血糖水平降低。总蛋白、白蛋白和总胆固醇在体内随时间逐渐降低。然后将猪分为4组，第1组为不含葡萄糖的灌流液，第2组为含2.5%葡萄糖的灌流液，第3组为不含葡萄糖但含胰岛素的灌流液，第4组为含2.5%葡萄糖和胰岛素的灌流液。结果，总蛋白和白蛋白随时间逐渐降低。用含葡萄糖的灌注液灌注的组的降低程度是轻度的。葡萄糖灌流液组血糖逐渐升高。胰岛素不影响血糖水平。总胆固醇随着时间的推移逐渐下降，然而，甘油三酯保持不变，而是在灌注过程中增加。在所有实验中，胰岛素在隔离灌注期间从未影响肝脏代谢。