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An experimental study of craniofacial bone degeneration in a heterotopic rat head transplant

大鼠异位换头颅面骨退变的实验研究

基本信息

批准号：
16591801
负责人：
YONEHARA Yoshiyuki
金额：
$ 1.92万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

It has been assumed that the formation of craniofacial bone requires synthetic growth of each piece of bone which is separated into several parts. However, it is not known whether the factors which determine the growth exist in the bone itself, the periostea, or surrounding soft tissues. The purpose of the present study is to confirm the location of the factor(s) which determine craniofacial bone formation by creating a craniofacial bone model which can grow without being affected by soft tissues. The descending aorta and inferior vena cava of a 2-week old Wistar rat were anastomosed to the femoral vessels in the inguinal region of a mature rat under a microscope. The transplanted rats were labeled one and two weeks after the transplantation, and sacrificed after 3 weeks for sample preparation. A 3-week old rat without surgery was used as a control, and labeling was performed in the same manner. Measurements were conducted in the vicinity of sutured areas in the nasal bone and premaxil … More la, and each area was divided into the front, middle, and back regions. For the nasal bone, the volume of bone accretion was measured, while the distance of bone accretion was measured for the premaxilla. At the end, the bone volume was determined in the Cole's HE staining. Significant differences were observed in the morphology of bone accretion in the nasal bone, while no difference was observed in the amount (volume) of bone accretion. In the premaxilla, differences were also observed in the morphology of bone accretion. The largest amount of bone volume was observed in the control. The results of the present study suggested that a gene which determines the "volume" of bone formation upon growth was expressed in the cells in hard tissues, such as periosteum. On the other hand, it, was also suggested that cells in the surrounding soft tissues take an important role for the expression of a gene which determines the "shape~ of bones. And, in clinical cases, we made examinations of bone regenerative courses after treatment of facial bone fracture and rhinoplasty in cleft lip nasal deformity. Less

据推测，颅面骨的形成需要每一块骨的合成生长，每一块骨被分成几个部分。然而，目前尚不清楚决定生长的因素是否存在于骨本身、骨膜或周围软组织中。本研究的目的是通过建立一个可以生长而不受软组织影响的颅面骨模型来确认决定颅面骨形成的因素的位置。在显微镜下，将2周龄Wistar大鼠的降主动脉和下腔静脉与成年大鼠腹股沟区的股血管吻合。在移植后1周和2周对移植大鼠进行标记，并在3周后处死以制备样品。将未手术的3周龄大鼠用作对照，并以相同的方式进行标记。在鼻骨和前颌骨缝合区域附近进行测量 ...更多信息 la，每个区域分为前、中、后区。对于鼻骨，测量骨生长的体积，而对于前上颌骨，测量骨生长的距离。最后，通过科尔氏HE染色测定骨体积。在鼻骨中观察到的骨增生的形态学上的显著差异，而在骨增生的量（体积）上没有观察到差异。在前上颌骨中，也观察到不同的骨增生的形态。在对照组中观察到最大量的骨体积。本研究的结果表明，在硬组织（如骨膜）的细胞中表达了一种决定生长时骨形成"体积"的基因。另一方面，也有人提出，周围软组织中的细胞对决定骨形状的基因的表达起着重要作用。并在临床病例中，对唇裂鼻畸形面骨骨折及鼻整形术后的骨再生过程进行了检测。少