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Development of grafting bone tissue from periosteum using in vivo tissue engineering

利用体内组织工程从骨膜移植骨组织的发展

基本信息

批准号：
14571721
负责人：
YONEHARA Yoshiyuki
金额：
$ 2.18万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571721/
关键词：
perlosteum bone bone formation In vivo tissue engineering artificial material bone reconstruction collagen

项目摘要

Autogenous bone graft still remains the standard for bone reconstructive techniques. However, because of concerns associated with donor site morbidity and because there is only a limited supply of. autogenous bone available, there has been intense interest in the development of bone graft substitutes. The estrogenic capacity of periosteum can also be used to induce bone. Vascularized periosteum has been shown to contribute to osteogenesis in bone development with variable success. The purpose of our study was to observe a newly formed bone fortune by using a vascularized periosteal flap and to evaluate osteogenic capacity of vascularized periosteum with atelo-collagen sponge. Both hind limbs of rats were used to construct vascularized periosteal flaps. A vascularized periosteal flap was dissected off the surface of the tibia and fibula. The flap was tubed on itseW with, the periosteal layer inside. In the atelo-collagen sponge graft case, the tube of right side leg containing atelo-collagen sponge. Bone formation Was evaluated radiographically and histlogically. Good bone formations were radiographically observed in the vascularized periosteal flaps 5 days after operation. Length of newly formed bone gradually increased still 8 weeks after operation. After B weeks operation, newly formed bone length gradually decreased. Width of newly formed bone gradually increased still 4 weeks after operation. After 4 weeks operation, newly formed bone width gradually decreased. On histological evaluation, woven bone formation was observed in the vascularized periosteal flap 5 days after operation. After 2 weeks post operation, lamellar bone formation was observed. New bone formation increased both radiographically and histlogically from 5 days td 4 weeks in the periosteal flaps containing atelo-collage sponge. After 4 weeks operation, there was no significant more bone formation radiographically and histlogicafly in the group with atelo-collagen sponge graft.

自体骨移植仍然是骨重建技术的标准。然而，由于与供体部位发病率相关的担忧，并且因为只有有限的供应。由于自体骨的存在，人们对骨移植替代物的开发产生了浓厚的兴趣。骨膜的雌激素样能力也可用于诱导成骨。血管化骨膜已被证明有助于骨发育中的骨生成，并具有不同的成功率。本研究的目的是观察带血管骨膜瓣的新骨形成情况，并评价带血管骨膜与端胶原海绵的成骨能力。用大鼠双后肢构建带血管蒂骨膜瓣。从胫骨和腓骨表面切下带血管蒂的骨膜瓣。皮瓣上置管，内有骨膜层。在缺端胶原海绵移植病例中，右侧腿的管含有缺端胶原海绵。骨形成通过放射学和组织学进行评价。术后第5天，X线片显示带血管骨膜瓣骨形成良好。术后8周新生骨长度逐渐增加。术后B周，新生骨长度逐渐减少。术后4周新生骨宽度逐渐增加。术后4周，新生骨宽度逐渐减小。术后5天，带血管骨膜瓣内可见编织骨形成。术后2周，可见板层骨形成。在含缺骨胶原海绵的骨膜瓣中，从第5天到第4周，新骨形成在放射学上和组织学上均增加。术后4周，X线片和组织学检查显示，端胶原海绵组骨形成量无明显增加。