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The role of cell cortex structures in mechanical stress signal transduction in osteoblasts.

细胞皮层结构在成骨细胞机械应力信号转导中的作用。

基本信息

批准号：
16591822
负责人：
IKEGAME Mika
金额：
$ 2.18万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591822/
关键词：
mechanical stress osteoblasts cytoskeleton cell cortex ultrastructure histochemistr actin bone suture

项目摘要

Tensile stress (TS) has been shown to stimulate osteogenesis in the cranial suture of mouse calvaria. In order to clarify the role of the adhesion complex and cytoskeleton in this mechanism, the morphological changes in such stressed suture cells were investigated.TS was applied to the cranial suture of neonatal mouse calvaria in culture. Some were stained with Alexa phalloidin and their actin fibers observed with confocal laser scanning microscopy, with others being observed with TEM. The results show that in the mature osteoblasts in the suture, actin was accumulated in the cell cortex. In the preosteoblastic cells and fibroblastic cells, actin was observed as stress fiber-like structures. Those were thicker in fibroblastic cells than in the preosteoblastic cells. With the tensile stress for 3 or 6 hours, all the cells in the sutures were stretched and elongated in the direction of the tensile stress. Morphological change of actin-cytoskeleton was observed consistent with the change of the cell shape. Thickness of the actin stress fiber-like structure and actin cell cortex were increased in the cells of the tensile stressed suture for 6 hours, but they were not clearly increased in the 3 hour-stressed group. Cadherin, one of the cell adhesion molecules, was observed along the cell cortex of osteoblasts at any experimental time course. On the other hand, immunological activities of β-catenin and vinculin became intense along the cell cortex in the osteoblasts of the tensile stressed groups.

拉伸应力(TS)可以刺激小鼠颅骨缝内的成骨。为了阐明黏附复合体和细胞骨架在这一机制中的作用，研究了这种应力性缝合细胞的形态变化。将TS应用于培养的新生小鼠颅骨缝。其中一些用Alexa phalloidin染色，用激光共聚焦显微镜观察其肌动蛋白纤维，用透射电子显微镜观察另一些。结果表明，在缝合线内成熟的成骨细胞中，肌动蛋白在细胞皮质中积聚。在成骨前细胞和成纤维细胞中，肌动蛋白呈应力纤维样结构。成纤维细胞比成骨前细胞更厚。在张应力作用3~6h后，缝线内的细胞均沿张应力方向拉伸、延长。肌动蛋白细胞骨架的形态变化与细胞形态的变化相一致。张力缝合6h后细胞内肌动蛋白应力性纤维样结构和肌动蛋白细胞皮质厚度增加，3h应激组增加不明显。钙粘附素是一种细胞黏附分子，在任何实验时间段均可沿成骨细胞的细胞皮质观察到。另一方面，张力应激组成骨细胞中β-连环蛋白和纽蛋白的免疫活性沿细胞皮质增强。