Possible roles of adhesion complex and cytoskeleton as a mechano-receptor in osteoblasts.

粘附复合物和细胞骨架作为成骨细胞中的机械受体的可能作用。

• 批准号: 14571729
• 负责人: IKEGAME Mika
• 金额: $ 1.86万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571729/
• 关键词: Stress, Mechanical; Osteoblasts; Cytoskeleton; Adhesion complex; Cell cortex; Ultrastructure

Tensile stress(TS) has been shown, to stimulate osteogenesis in the cranial suture of mouse calvaria. In order to clarify the role of the adhesion complex and cytoskeleton in this mechanism, the morphological changes in such stressed suture cells was investigated.TS was applied to the cranial suture of neonatal mouse calvaria in culture. Some were stained with Alexa phalloidin and their actin fibers observed with con focal laser scanning microscopy, with others being observed with TEM. The results show that in the osteogenic layers of the cranial suture, TS causes actin-fiber morphological change, principally in the osteoblasts located at the bone edge, as well as in the cells located in the center of the suture.Furthermore, we previously demonstrated that TS induced protein production, as well as the gene expression of α-adaptin C following the initiation of loading. In situ hybridization and immunohistochemical analysis revealed that the induction of α-adaptin C mostly occurred in fibroblastic cells in the sutures, suggesting that it precedes TS-induced osteoblast differentiation. Consistent with this result, TS significantly increased the number of coated pits and coated vesicles in the undifferentiated fibroblastic cells but not in the osteoblastic cells around calvarial bones. These results, taken together, suggest that TS accelerates osteoblast differentiation and osteogenesis, possibly through the induction of the α-adaptin C expression and consequent activation of receptor-mediated endocytosis.

已经显示了拉伸应力（TS），以刺激小鼠瓦尔瓦里亚颅缝合中的成骨。为了阐明粘合剂复合物和细胞骨架在该机制中的作用，研究了这种应力缝合细胞的形态变化。有些用Alexa鬼笔环肽染色，其肌动蛋白纤维用CON焦点激光扫描显微镜观察到，另一些则观察到TEM。结果表明，在颅骨缝合的成骨层中，TS导致肌动蛋白纤维形态变化，主要是在位于骨边缘的成骨细胞中，以及位于缝合线中心的细胞中。原位杂交和免疫组织化学分析表明，α-蛋白蛋白C的诱导主要发生在缝合线中的成纤维细胞中，这表明它先于TS诱导的成骨细胞分化。与此结果一致，TS显着增加了未分化的成纤维细胞中涂层坑和涂层蔬菜的数量，但在成骨细胞中钙细胞周围的骨膜周围骨骼的数量显着增加了。这些结果共同表明，TS加速了成骨细胞的分化和成骨，可能是通过诱导α-适应素C的表达以及随之而来的受体介导的内吞作用的激活。

项目成果

Shimomura.J, Ishibashi O, Ikegame M, YoshizawQ T, Ejiri S, Noda T, Kawashima H: "Tensile stress induces α-adaptin C production in mouse calvariae in an organ culture : possible involvement of endbcytosis in mechanical stress-stimulated osteoblast differen

Shimomura.J、Ishibashi O、Ikegame M、YoshizawQ T、Ejiri S、Noda T、Kawashima H：“拉伸应力诱导器官培养中小鼠颅骨中 α-适应素 C 的产生：可能涉及机械应力刺激的成骨细胞中的胞吞作用不同

Shimomura, J.: "Tensile stress induces α-adaptin C production in mouse calvariae in an organ culture : possible imvolvement of endocytosis in mechanical stress-stimulated osteoblast differentiation"Journal of Cellular Physiology. 195. 488-496 (2003)

Shimomura, J.：“拉伸应力诱导器官培养中小鼠颅骨产生 α-适应素 C：内吞作用可能参与机械应力刺激的成骨细胞分化”，《细胞生理学杂志》195. 488-496 (2003)。

Shimomura, J.: "Tensile stress induces α-adaptin C production in mouse calvariae in an organ culture -The possible imvolvement of endocytosis in mechanical stress-stimulated osteoblast"Journal of Cellular Physiology. (発表予定).

Shimomura, J.：“拉伸应力诱导器官培养中小鼠颅骨产生 α-适应素 C - 机械应力刺激的成骨细胞内吞作用的可能参与”《细胞生理学杂志》（待出版）。