Possible roles of adhesion complex and cytoskeleton as a mechano-receptor in osteoblasts.

粘附复合物和细胞骨架作为成骨细胞中的机械受体的可能作用。

• 批准号: 14571729
• 负责人: IKEGAME Mika
• 金额: $ 1.86万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571729/
• 关键词: Stress, Mechanical; Osteoblasts; Cytoskeleton; Adhesion complex; Cell cortex; Ultrastructure

Tensile stress(TS) has been shown, to stimulate osteogenesis in the cranial suture of mouse calvaria. In order to clarify the role of the adhesion complex and cytoskeleton in this mechanism, the morphological changes in such stressed suture cells was investigated.TS was applied to the cranial suture of neonatal mouse calvaria in culture. Some were stained with Alexa phalloidin and their actin fibers observed with con focal laser scanning microscopy, with others being observed with TEM. The results show that in the osteogenic layers of the cranial suture, TS causes actin-fiber morphological change, principally in the osteoblasts located at the bone edge, as well as in the cells located in the center of the suture.Furthermore, we previously demonstrated that TS induced protein production, as well as the gene expression of α-adaptin C following the initiation of loading. In situ hybridization and immunohistochemical analysis revealed that the induction of α-adaptin C mostly occurred in fibroblastic cells in the sutures, suggesting that it precedes TS-induced osteoblast differentiation. Consistent with this result, TS significantly increased the number of coated pits and coated vesicles in the undifferentiated fibroblastic cells but not in the osteoblastic cells around calvarial bones. These results, taken together, suggest that TS accelerates osteoblast differentiation and osteogenesis, possibly through the induction of the α-adaptin C expression and consequent activation of receptor-mediated endocytosis.

张应力（TS）已被证明，刺激小鼠颅骨的颅缝中的骨生成。为了阐明粘附复合物和细胞骨架在这一机制中的作用，我们研究了这种应激缝细胞的形态学变化。有些用Alexa鬼笔环肽染色，用共聚焦激光扫描显微镜观察它们的肌动蛋白纤维，另一些用TEM观察。结果表明，在颅缝的成骨层中，TS引起肌动蛋白纤维的形态学改变，主要发生在骨边缘的成骨细胞中，也发生在骨缝中心的细胞中，此外，我们先前已经证明TS诱导蛋白质的产生以及α-adaptin C的基因表达。原位杂交和免疫组织化学分析显示，α-adaptin C的诱导主要发生在骨缝中的成纤维细胞，提示其先于TS诱导的成骨细胞分化。与此结果一致，TS显着增加未分化成纤维细胞中的包被小凹和包被小泡的数量，但在颅骨周围的成骨细胞中没有。综合这些结果，表明TS加速成骨细胞分化和成骨，可能是通过诱导α-adaptin C表达和随后激活受体介导的内吞作用。

项目成果

Shimomura.J, Ishibashi O, Ikegame M, YoshizawQ T, Ejiri S, Noda T, Kawashima H: "Tensile stress induces α-adaptin C production in mouse calvariae in an organ culture : possible involvement of endbcytosis in mechanical stress-stimulated osteoblast differen

Shimomura.J、Ishibashi O、Ikegame M、YoshizawQ T、Ejiri S、Noda T、Kawashima H：“拉伸应力诱导器官培养中小鼠颅骨中 α-适应素 C 的产生：可能涉及机械应力刺激的成骨细胞中的胞吞作用不同

Shimomura, J.: "Tensile stress induces α-adaptin C production in mouse calvariae in an organ culture : possible imvolvement of endocytosis in mechanical stress-stimulated osteoblast differentiation"Journal of Cellular Physiology. 195. 488-496 (2003)

Shimomura, J.：“拉伸应力诱导器官培养中小鼠颅骨产生 α-适应素 C：内吞作用可能参与机械应力刺激的成骨细胞分化”，《细胞生理学杂志》195. 488-496 (2003)。

Shimomura, J.: "Tensile stress induces α-adaptin C production in mouse calvariae in an organ culture -The possible imvolvement of endocytosis in mechanical stress-stimulated osteoblast"Journal of Cellular Physiology. (発表予定).

Shimomura, J.：“拉伸应力诱导器官培养中小鼠颅骨产生 α-适应素 C - 机械应力刺激的成骨细胞内吞作用的可能参与”《细胞生理学杂志》（待出版）。