樹状細胞ワクチン法を導入した特異的GVT療法の口腔がんへの応用

树突状细胞疫苗法特异性GVT疗法在口腔癌中的应用

• 批准号: 16591901
• 负责人: OHNO JUN
• 金额: $ 2.24万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591901/
• 关键词: GVT; ICAM-1; CTL; Melanoma; sialic acid; Dendritic cell; tumor immunology; ICAM-1; 口腔がん; がん免疫; GVHD; がんワクチン療法; 悪性黒色腫; 免疫組織化学; マウス

Graft-versus-tumor, GVT, is one of cellular immunotherapies for malignant tumors. In this study, we examined the effect of cytotoxic T cells (CTLs) presented melanoma antigens by dendritic cell (DC) to melanoma cell which have expressed intercellular adhesion molecule-1 (ICAM-1). Matured DCs were pulsed by a solution of B16 (melanoma cells) cells. CD8-positive T cells (CTLs) were stimulated by the co-culture of matured and pulsed DCs. After those procudures, the CTLs must be reactive with melanoma cells in vivo. As B16 cells were cultured in DMEM medium with interferon-gamma (IFN-g), those cells expressed ICAM-1 on the cell surface. The mouse melanoma model was made by the injection of B16 cell expressed ICAM-1 into the tongue. The controls were prepared as 1) the injection of DMEM insted of the B16 cell-injection, and 2) the injection of B16 cells without the expression with ICAM-1. The tongue with the injcetion of ICAM-1-B16 cells revealed remakable reduction of tumor growth, compared with the controls. These results suggested that the GVT, with combination of pulsed DCs and ICAM-1 expression on tumor cells, may be increased effects of tumor immunotherapy.

移植物抗肿瘤（GVT）是治疗恶性肿瘤的细胞免疫疗法之一。本研究探讨树突状细胞（DC）介导的细胞毒性T细胞（CTL）对表达细胞间粘附分子-1（ICAM-1）的黑色素瘤细胞的作用。用B16（黑色素瘤细胞）细胞溶液脉冲成熟DC。CD 8阳性T细胞（CTL）通过成熟和脉冲的DC的共培养来刺激。在这些过程之后，CTL必须在体内与黑素瘤细胞反应。当B16细胞在含有干扰素-γ（IFN-g）的DMEM培养基中培养时，这些细胞在细胞表面上表达ICAM-1。舌内注射表达ICAM-1的B16细胞制备小鼠黑色素瘤模型。对照制备为1）注射DMEM代替B16细胞注射，和2）注射不表达ICAM-1的B16细胞。舌部注射ICAM-1-B16细胞后肿瘤生长明显减少。这些结果表明，GVT与脉冲DC和肿瘤细胞表面ICAM-1表达的组合可能是肿瘤免疫治疗的增强效应。