樹状細胞ワクチン法を導入した特異的GVT療法の口腔がんへの応用

移植物抗肿瘤（Graft-versus-tumor， GVT）是恶性肿瘤的细胞免疫治疗方法之一。在这项研究中，我们检测了细胞毒性T细胞（ctl）通过树突状细胞（DC）向表达细胞间粘附分子-1 （ICAM-1）的黑色素瘤细胞呈递黑色素瘤抗原的作用。成熟的树突状细胞被B16（黑色素瘤细胞）细胞溶液脉冲。cd8阳性T细胞（ctl）被成熟的和脉冲的dc共培养刺激。在这些程序之后，ctl必须在体内与黑色素瘤细胞反应。B16细胞在含干扰素- γ (IFN-g)的DMEM培养基中培养，细胞表面表达ICAM-1。将表达ICAM-1的B16细胞注入舌部，制备小鼠黑色素瘤模型。对照组为：1)注射DMEM代替B16细胞注射，2)注射不表达ICAM-1的B16细胞。与对照组相比，注射ICAM-1-B16细胞的舌部肿瘤生长明显减少。提示GVT结合脉冲dc和ICAM-1在肿瘤细胞上的表达，可能会增加肿瘤免疫治疗的效果。

Graft-versus-tumor, GVT, is one of cellular immunotherapies for malignant tumors. In this study, we examined the effect of cytotoxic T cells (CTLs) presented melanoma antigens by dendritic cell (DC) to melanoma cell which have expressed intercellular adhesion molecule-1 (ICAM-1). Matured DCs were pulsed by a solution of B16 (melanoma cells) cells. CD8-positive T cells (CTLs) were stimulated by the co-culture of matured and pulsed DCs. After those procudures, the CTLs must be reactive with melanoma cells in vivo. As B16 cells were cultured in DMEM medium with interferon-gamma (IFN-g), those cells expressed ICAM-1 on the cell surface. The mouse melanoma model was made by the injection of B16 cell expressed ICAM-1 into the tongue. The controls were prepared as 1) the injection of DMEM insted of the B16 cell-injection, and 2) the injection of B16 cells without the expression with ICAM-1. The tongue with the injcetion of ICAM-1-B16 cells revealed remakable reduction of tumor growth, compared with the controls. These results suggested that the GVT, with combination of pulsed DCs and ICAM-1 expression on tumor cells, may be increased effects of tumor immunotherapy.