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Influence of Enzymes on the Biofilm Formation by the Isolated Bacteria from the Infected Root Canals.

酶对感染根管中分离细菌生物膜形成的影响。

基本信息

批准号：
16591927
负责人：
HOSOYA Noriyasu
金额：
$ 2.37万
依托单位：
Tsurumi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591927/
关键词：
Biofilm Enzyme Bacteria of infected root canal Electrophoresis 根管分離細菌

项目摘要

Objective : Biofilm formation in root canals is one of the main causes of intractable infected root canal cases. Biofilm is formed by Extracellular Polymeric Substances (EPS), which the bacteria produce and secrete outside cells. Structures and features of EPS differ depending on the bacteria forming them. In this study, we observed the influence of enzymes on EPS production by Porphyromonas gingivalis, and Staphylococcus aureus isolated from the infectedMaterials and Methods : Inhibition effect ; A cell disk coated with collagen type I was suspended in nutrient medium containing pectinase (1%) or dextranase (1%), and cultured in an aerobic environment for 7 days. The surface of the cell disk was observed with a scanning electron microscope (SEM). Destructive effect; After culturing the cell disk in the medium for 7 days, pectinase and dextranase were added to the medium to reach a density of 1%. These were cultured for another 24 hours. The medium without enzyme was used as the control in bothResults and Conclusion : P.gingivalis, and S.aureus formed biofilms in all control groups. However, the formation of biofilm was different between bacteria. There was less EPS production by both bacteria cultured in medium containing 1% pectinase, compared with the control group. However, EPS production in medium containing 1% dextranase and in the control group was not different. The destructive effect of the enzyme for EPS was not evident. It is assumed that pectinase affects the process of EPS production by P.gingivalis and S.aureus.

目的：根管内生物膜形成是难治性根管感染病例的主要原因之一。生物膜是由细菌在细胞外产生和分泌的细胞外聚合物质（EPS）形成的。EPS的结构和特征因形成它们的细菌而异。在本研究中，我们观察了酶对牙龈卟啉单胞菌和金黄色葡萄球菌产生EPS的影响。将包被I型胶原蛋白的细胞盘悬浮在含有1%果胶酶或1%葡聚糖酶的营养培养基中，在有氧环境中培养7天。用扫描电子显微镜（SEM）观察细胞盘表面。破坏性的效果;细胞盘在培养基中培养7天后，在培养基中加入果胶酶和葡聚糖酶，使其密度达到1%。再培养24小时。结果与结论：各对照组牙龈假单胞菌和金黄色葡萄球菌均形成生物膜。然而，细菌之间形成的生物膜是不同的。在含有1%果胶酶的培养基中培养的两种细菌EPS产量均低于对照组。然而，在含有1%葡聚糖酶的培养基中，EPS的产量与对照组没有差异。酶对EPS的破坏作用不明显。假设果胶酶影响牙龈假单胞菌和金黄色假单胞菌生产EPS的过程。