Development of optoelectronically active nerve adhesive for accelerating peripheral nerve repair

开发用于加速周围神经修复的光电活性神经粘合剂

• 批准号: 10811395
• 负责人: Bin Duan
• 金额: $ 43.73万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-22 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10811395
• 关键词: Acceleration; Adhesions; Adhesives; Affect; Air; Allergic Reaction; Amines; Autologous Transplantation; Axon; Basic Science; Behavior; Biocompatible Materials; Breathing; Catechols; Cell Proliferation; Cell Size; Cells; Cicatrix; Consumption; Covalent Interaction; Defect; Denervation; Development; Devices; Disease; Distal; Dopamine; Dryness; Electric Stimulation; Electricity; Environment; Extracellular Matrix; Fibrin Tissue Adhesive; Foreign-Body Reaction; Genes; Goals; Health Care Costs; Heart; Hyaluronic Acid; Hydrogels; In Vitro; Inflammatory; Injury; Isothiocyanates; Life; Light; Microsurgery; Modeling; Motor; Muscle; Muscular Atrophy; Natural regeneration; Nerve; Neurites; Neurons; Operative Surgical Procedures; Output; Patients; Performance; Peripheral; Peripheral Nerves; Peripheral nerve injury; Physiological; Procedures; Process; Proliferating; Property; Public Health; Quality of life; Quinones; Rationalization; Rattus; Recovery; Risk; Schwann Cells; Sensory; Silicon; Skeletal Muscle; Spinal Ganglia; Sulfhydryl Compounds; Surgical sutures; Swelling; System; Temperature; Testing; Thick; Thiourea; Time; Tissue Adhesives; Tissues; Work; axon growth; biomaterial compatibility; cell behavior; chronic pain; clinical application; cohesion; cytotoxicity; design; disability; fabrication; functional disability; healing; implantation; improved; in vitro activity; in vivo; injury and repair; innovation; light intensity; mechanical properties; meter; motor function recovery; motor impairment; nerve damage; nerve gap; nerve injury; next generation; novel; operation; peripheral nerve regeneration; peripheral nerve repair; peripheral nerve transection; protein expression; regeneration function; repaired; response; sciatic nerve; social; submicron; tissue injury; tissue regeneration; transmission process; wireless

Project Summary Peripheral nerve (PN) injury represents a major public health problem that leads to functional impairment and permanent disability. Microsurgical suturing, standard approach for long-gap PN repair, is a time-consuming procedure and causes nerve damage, inflammatory foreign body reactions, and scar formation, which delay the PN regeneration. As potential alternatives, tissue adhesives have been developed to reduce operation time and avoid secondary damages. However, current commercially available tissue adhesives, like fibrin glue and others, are far from ideal, considering cytotoxicity, tissue compression due to extensive swelling, and poor mechanical properties. We have developed a novel dual network nerve adhesive (NA) consisting of catechol modified hyaluronic acid and decellularized peripheral nerve matrix hydrogels. Our NAs illustrated significantly higher adhesion strength and adhesion force, compared to catechol modified HA only and commercial fibrin glue. The NAs supported Schwann cell proliferation and improved PN repair after transection injury comparing to fibrin glue. However, both sensory and motor functions were still incompletely recovered after microsuturing or NA repair in the transected and long-gap nerve injury models. In this proposal, we will further incorporate innovative optoelectronic biomaterials (i.e., Si based μ-solar cells) within the NA to develop next generation of optoelectronically active NAs (optoENAs) for long-gap PN injury regeneration. The Si based μ-solar cells are in micrometer size, biocompatible, biodegradable, and photo-stimulable to generate sufficient electrical output. The specific aims of the studies are (1) to develop functional optoENA and determine how the size and concentration of μ-solar cells affect NA properties and PN related cell behaviors; and (2) to determine whether and how optoENAs expedite surgical procedures, facilitate autograft implantation, and promote long-gap PN repair in a rat model. This proposal will develop a novel and clinically applicable tissue adhesive with enhanced adhesive performance and optoelectronic properties for improving healing and regeneration of long-gap PN injury.

项目总结