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A method to measure time-varying viscoelastic properties using nanoscale vibration in intact cardiac myocytes

一种利用完整心肌细胞的纳米级振动测量时变粘弹性特性的方法

基本信息

批准号：
17500325
负责人：
SHISHIDO Toshiaki
金额：
$ 2.24万
依托单位：
National Cardiovascular Center Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500325/
关键词：
ventricle cardiomyocyte heart disease biomechanics contractility time-varying elastance model 可変弾性モデル

项目摘要

1.To test whether the minute vibration technique can separate viscosity and elasticity of the cardiac myocyte, we quantitatively assessed the regional myocardial elasticity and viscosity using this technique. Time-varying regional myocardial viscosity as well as elastance increased from diastole to systole and linearly correlated with time-varying ventricular elastance.2.We applied this technique in the regional myocardium to an isolated cardiac myocyte. Nanoscale vibration was generated by atomic force microscope. However, there was only a small difference in the calculated viscoelasticity between the end-systolic and the diastolic phases. Moreover, there was no change of this difference between systole and diastole under the unstressed conditions after the contractility was enhanced by dobutamine.3.We developed a new device to stretch an isolated cardiac myocyte and tried to measure the viscoelasticity of a cardiac myocyte under the loading conditions. However, the isolated cardiac m … More yocytes were easily peeled off from the culture sheet because it was difficult to stretch the sheet uniformly.4.Maximizing yield of viable cardiac myocytes is most important in the processing of small human myocardial biopsies. We established the conditions of protease and collagenase digestion of ventricular myocardium to obtain viable cardiac myocytes in adults rats. The combination of 4U/ml protease (type XXIV) and 400U/ml collagenase-A was more effective than either enzyme alone in increasing viable cardiac myocytes.5.We developed a computer model to relate cardiomyocyte-level viscoelasticity to whole-heart contractility. This model was based on Laplace's law and applied across a multilayer of myocardium. We could integrate the stress-strain relationship in cell-level to the pressure-volume relationship in heart-level.In the next phase, we are planning to measure the viscoelasticity of an isolated cardiac myocyte by the cell fixation technique that uses a pair of carbon fibers to clamp a cardiac myocyte. Less

1.为验证微振动技术能否分离心肌细胞的粘度和弹性，本研究应用微振动技术定量测定了局部心肌的弹性和粘度。时变局部心肌粘度和弹性从舒张期到收缩期逐渐增加，并与时变心室弹性呈线性相关。2.我们将此技术应用于离体心肌细胞的局部心肌。利用原子力显微镜产生纳米级振动。然而，在收缩末期和舒张期之间的计算粘弹性只有很小的差异。在无应力状态下，多巴酚丁胺增强心肌细胞的收缩力后，收缩期与舒张期的这种差异没有改变。3.我们研制了一种新的心肌细胞拉伸装置，并尝试测量了心肌细胞在负荷状态下的粘弹性。然而，孤立的心脏M ...更多信息由于难以均匀地拉伸培养片，因此很容易从培养片上剥离出心肌细胞。4.在处理小型人心肌活组织检查中，最大限度地提高活心肌细胞的产量是最重要的。我们建立了成年大鼠心室肌蛋白酶和胶原酶消化的条件，以获得存活的心肌细胞。4U/ml蛋白酶（XXIV型）和400 U/ml胶原酶-A的组合比单独使用任何一种酶在增加存活心肌细胞方面更有效。5.我们开发了一个计算机模型，将心肌细胞水平的粘弹性与整个心脏的收缩力联系起来。该模型基于拉普拉斯定律并应用于多层心肌。我们可以将细胞水平的应力-应变关系整合到心脏水平的压力-容积关系中。在下一阶段，我们计划通过使用一对碳纤维夹持心肌细胞的细胞固定技术来测量分离的心肌细胞的粘弹性。少

项目成果

期刊论文数量（21）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effects of Ca2+ channel antagonists on nerve stimulation-induced and ischemia-induced myocardial interstitial acetylcholine release in cats

Ca2+通道拮抗剂对猫神经刺激和缺血诱导的心肌间质乙酰胆碱释放的影响

DOI：
发表时间：
2007
期刊：
Am J Physiol Heart Circ Physiol 291
影响因子：
0
作者：
Kawada T;et al.
通讯作者：
et al.

Automated drug delivery system to control systemic arterial pressure, cardiac output, and left heart filling pressure in acute decompensated heart failure.

自动给药系统可控制急性失代偿性心力衰竭的全身动脉压、心输出量和左心充盈压。

DOI：
发表时间：
2006
期刊：
J Appl Physiol. 100
影响因子：
0
作者：
T.Ito et al.;M.Kawashita.et al.;Y.Jujimura;Kazuhiro NAKASHIMA et al.;Uemura K et al.
通讯作者：
Uemura K et al.

Dynamic characteristics of carotid sinus pressure-nerve activity transduction in rabbits.

家兔颈动脉窦压力-神经活动传导的动态特征。

DOI：
发表时间：
2005
期刊：
Jpn J Physiol. 55
影响因子：
0
作者：
Kawada T et al.
通讯作者：
Kawada T et al.

Myocardial interstitial choline and glutamate levels during acute myocardial ischaemia and local ouabain administration

DOI：
10.1111/j.1365-201x.2005.01444.x
发表时间：
2005-07-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
0
作者：
Kawada, T;Yamazaki, T;Sugimachi, M
通讯作者：
Sugimachi, M

Sympathetic neural regulation of heart rate is robust against high plasma catecholamines.

DOI：
10.2170/physiolsci.rp006006
发表时间：
2006-06
期刊：
The journal of physiological sciences : JPS
影响因子：
0
作者：
T. Kawada;T. Miyamoto;Yuichiro Miyoshi;S. Yamaguchi;Y. Tanabe;A. Kamiya;T. Shishido;M. Sugimachi
通讯作者：
T. Kawada;T. Miyamoto;Yuichiro Miyoshi;S. Yamaguchi;Y. Tanabe;A. Kamiya;T. Shishido;M. Sugimachi