Elucidation of the mechanism that therapeutic exercise effectively inhibits the aggravation of progressive renal disease in diabetes mellitus.

阐明治疗性运动有效抑制糖尿病进展性肾病恶化的​​机制。

基本信息

  • 批准号:
    17500343
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2005
  • 资助国家:
    日本
  • 起止时间:
    2005 至 2006
  • 项目状态:
    已结题

项目摘要

To assess the renal effects of chronic exercise (EX) in a rat model of diabetic nephropathy (DN) and the benefits of combined EX and angiotensin II type-1 receptor antagonist, losartan (LOS).Heminephrectomized Goto-Kakizaki rats were fed a high-caloric diet and had free access to 30% sucrose solution to develop DN. The rats were divided into 4 groups: 1) no EX (C); 2) EX with treadmill running (20m/min, 60min/day, 5days/week); 3) LOS(5mg/kg/day); 4) EX+LOS, and the rats were treated for 12 weeks. At the end of the experiment, remnant kidneys were removed, and were analyzed for histopathology, and for immunohistologically using antibodies against ED-1, alpha-smooth muscle actin (SMA) and Wilms' tumor (WT)-1.Systolic blood pressure (SBP) in the LOS and EX+LOS groups was significantly lower than that in the C and EX groups. EX, EX+LOS and LOS blunted increases in proteinuria. The index of glomerular sclerosis (IGS) and the relative interstitial volume of the renal cortex were significantly improved in the EX, EX+LOS and LOS groups than those in the C group. Particularly, the IGS, the expressions of ED-1 and alpha-SMA in the glomerulus were the lowest, and the number of WT-1 positive cells was the highest in the EX+LOS group. The ability of endurance running in the EX and EX+LOS groups was significantly stronger than that in the LOS and C groups.These results suggested that EX and LOS have renoprotective effects, and that the effects of EX were not related to the SBP reduction. These results also suggested that the simultaneous treatment with EX and LOS provided greater renoprotective effect than that of LOS alone and may affect the macrophage infiltration, the mesangial activation, and the podocyte loss in this rat model of DN. They also suggested that EX has the effect which enhances endurance without renal complications in this rat model.
为探讨慢性运动(EX)对糖尿病肾病(DN)大鼠肾脏的影响以及EX与血管紧张素Ⅱ 1型受体拮抗剂氯沙坦(LOS)联合应用的益处,采用半肾切除的Goto-Kakizaki大鼠,通过高热量饮食和自由饮用30%蔗糖溶液来建立DN模型。将大鼠分为4组:1)无EX组(C组); 2)EX+跑台运动组(20 m/min,60 min/d,5d/周); 3)LOS组(5 mg/kg/d); 4)EX+LOS组,共治疗12周。实验结束后,取残余肾脏进行组织病理学分析,并应用抗艾德-1、抗α-平滑肌肌动蛋白(SMA)和抗肾母细胞瘤(WT)-1抗体进行免疫组织化学分析。EX、EX+LOS和LOS使蛋白尿的增加减弱。EX组、EX+LOS组和LOS组肾小球硬化指数(IGS)和肾皮质相对间质体积均较C组明显改善。其中EX+LOS组肾小球内IGS、艾德-1和α-SMA表达最低,WT-1阳性细胞数最多。EX组和EX+LOS组的耐力跑能力明显强于LOS组和C组,提示EX和LOS具有肾脏保护作用,EX的作用与降低SBP无关。这些结果也表明,EX和LOS同时治疗提供了更大的肾脏保护作用比单独的LOS,并可能影响巨噬细胞浸润,系膜激活,足细胞丢失在这个大鼠模型的DN。他们还表明,EX具有在该大鼠模型中增强耐力而无肾脏并发症的作用。

项目成果

期刊论文数量(38)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
障害と活動の測定・評価ハンドブック-機能から QOL まで
残疾和活动测量和评估手册 - 从功能到生活质量
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    金澤雅之;他;金澤雅之
  • 通讯作者:
    金澤雅之
Renoprotective and antihypertensive effect o combination therapy with a low protein diet, long- term exercise and angiotensin II recepto : antagonist in rats with renal ablation.
低蛋白饮食、长期运动和血管紧张素 II 受体联合治疗的肾脏保护和抗高血压作用:肾脏消融大鼠体内的拮抗剂。
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sasaki Y;et al.
  • 通讯作者:
    et al.
Combination of exercise and enalapril enhance renoprotective and peripheral effects in rats with renal ablation.
运动和依那普利的结合可增强肾脏消融大鼠的肾脏保护和外周作用。
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kanazawa M;et al.
  • 通讯作者:
    et al.
Expression of cytochrome P-450 4 enzymes in the kidney and liver : Regulation by PPAR and species-differene between rat and human.
细胞色素 P-450 4 酶在肾脏和肝脏中的表达:PPAR 的调节以及大鼠和人类之间的物种差异。
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ito O;et al.
  • 通讯作者:
    et al.
Effect of low-voltage electrical stimulation on angiogenic growth factors in ischaemic rat skeletal muscle
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KANAZAWA Masayuki其他文献

KANAZAWA Masayuki的其他文献

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{{ truncateString('KANAZAWA Masayuki', 18)}}的其他基金

The mechanism elucidation of renal rehabilitation in a progressive renal disease
进行性肾病肾康复的机制阐明
  • 批准号:
    21500463
  • 财政年份:
    2009
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of the mechanism that combination therapy with long-term exercise, G-CSF and nutrition therapy effectively inhibits the aggravation of progressive renal disease
阐明长期运动、G-CSF和营养疗法联合治疗有效抑制进行性肾病加重的机制
  • 批准号:
    19500436
  • 财政年份:
    2007
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of the mechanism that rehabilitation therapeutic exercise protects myocardium
阐明康复治疗运动保护心肌的机制
  • 批准号:
    15500358
  • 财政年份:
    2003
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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DMGV and the AGXT2 pathway in chronic exercise-induced cardiometabolic adaptations.
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  • 批准号:
    10214687
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    2020
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DMGV 和 AGXT2 通路在慢性运动引起的心脏代谢适应中的作用。
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  • 财政年份:
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DMGV and the AGXT2 pathway in chronic exercise-induced cardiometabolic adaptations.
DMGV 和 AGXT2 通路在慢性运动引起的心脏代谢适应中的作用。
  • 批准号:
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  • 财政年份:
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DMGV and the AGXT2 pathway in chronic exercise-induced cardiometabolic adaptations.
DMGV 和 AGXT2 通路在慢性运动引起的心脏代谢适应中的作用。
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Roles of AMPK signaling on memory extinction by chronic exercise in rats with low spatial learning ability
AMPK 信号传导对空间学习能力低下大鼠慢性运动记忆消退的作用
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    16K01748
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长期运动的抗衰老作用机制
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