Construction of self-assembled membranes that can support lipid bilayers

构建可支持脂质双层的自组装膜

• 批准号: 17550141
• 负责人: SHIBAKAMI Motonari
• 金额: $ 1.15万
• 依托单位: National Institute of Advanced Industrial Science and Technology (AIST)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17550141/
• 关键词: self-assembled membranes; lipid membranes; 基板

The primary aim of this project is to create self-assembled membranes (SAMs) that can support lipid bilayers. First, t investigator synthesized the lipooligomers containing thiol group by conjugating a cyclic lipid and oligoethyleneglycol. Th SAMS were prepared by dipping gold substrate (quartz crystal with gold electrode) into a mixture of the lipooligomer a thioalcohol with various molar ratios.To examine if the SAMs would immobilize lipid bilayers in aqueous solution, the investigator used quartz crystal microbalance as a detecting tool. Namely, liposome dispersion was placed on the SAM attached to gold electrode on a quartz crystal, and measured frequency veering. The decrease of frequency indicates immobilization of lipid bilayers on the SAM. The investigator examined various SAMS, found that the membranes that are composed of cyclic lipid lipooligomer and thioalcohol with a ratio of 2:98 effectively immobilized.

该项目的主要目标是创造能够支持脂质双层的自组装膜(SAM)。首先，研究人员通过将环状脂质与低聚乙二醇偶联制得了含硫基脂低聚物。将金基质(含金电极的石英晶体)浸入不同摩尔比的脂低聚物硫醇混合物中制备自组装膜，研究人员使用石英晶体微天平作为检测工具，考察自组装膜在水溶液中是否能固定化脂质双层。即将脂质体分散在石英晶体上金电极上的SAM上，测量频率偏转。频率的降低表明脂质双层被固定在SAM上。研究人员检查了各种自组装膜，发现由环状脂质低聚物和硫醇以2：98的比例组成的膜有效地固定化了。

项目成果

環状エーテル・アミド化合物

环醚/酰胺化合物

• 发表时间: 2006