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Development of brain controlling mechanism of sexual orientation

大脑性取向控制机制的发展

基本信息

批准号：
17570054
负责人：
ITOH Masanori
金额：
$ 2.24万
依托单位：
St. Marianna University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570054/
关键词：
behavior brain serotonin insect

项目摘要

Pairs of adult male crickets fight and immediately determine their dominant and subordinate statuses. The dominant male repeatedly produces aggressive (rival) song by rubbing his forewings together. Previous studies have suggested that aggressive song emitted by a dominant male prevents reduction of serotonin (5-hydroxytryptamine ; 5-HT) levels in his own brain. To test this hypothesis, we removed the plectrum (a sound-producing structure in the forewing) from male crickets and measured their brain 5-HT levels immediately after a 10-min aggressive interaction, using high-performance liquid chromatography and immunohistochemistry. Pairs of plectrum-removed males fought and established clear dominant-subordinate relationships, like the case of intact males. The plectrum-removed dominant males frequently rubbed their forewings together, but were unable to produce song. Brain 5-HT levels in the plectrum-removed males were significantly reduced immediately after the aggressive interaction, regardless of their dominance status. The decrease in 5-HT primarily occurred in the central body of the brain. In contrast, in pairs of intact males, aggressive interaction reduced brain 5-HT levels in the subordinate males, but not in the dominant males. Plectrum removal alone did not affect the brain 5-HT levels. These findings demonstrate that aggressive song emitted by a dominant male cricket, but not rotation opening/closing movements of his forewings, prevents reduction of 5-HT levels in his own brain (especially in the central body). It is strongly suggested that in aggressive male crickets, self-generated song affects brain functions such as synthesis and/or degradation of 5-HT in neurons of the central body, resulting in alteration of synaptic transmission.

成对的成年雄性蟋蟀打斗并立即确定它们的统治地位和从属地位。占主导地位的雄性通过摩擦前翅反复发出攻击性（竞争性）的歌声。先前的研究表明，占主导地位的雄性发出的攻击性歌曲会阻止其大脑中血清素（5-羟色胺；5-HT）水平的降低。为了验证这一假设，我们去除了雄性蟋蟀的拨片（前翅中的发声结构），并使用高效液相色谱和免疫组织化学在 10 分钟的攻击性互动后立即测量了它们的大脑 5-HT 水平。一对被去除拨片的雄性会互相争斗，并建立起明确的主从关系，就像完好无损的雄性一样。去除了拨片的雄性雄性经常摩擦前翅，但无法发出声音。去除拨片的雄性大脑中的 5-HT 水平在攻击性互动后立即显着降低，无论其统治地位如何。 5-HT的减少主要发生在大脑的中央体。相比之下，在成对的完整雄性中，攻击性互动会降低从属雄性的大脑 5-HT 水平，但不会降低占主导地位的雄性的大脑 5-HT 水平。单独去除拨片并不影响大脑 5-HT 水平。这些发现表明，占主导地位的雄性蟋蟀发出的攻击性歌曲，而不是其前翅的旋转打开/关闭运动，可以防止其大脑（尤其是中央身体）中 5-HT 水平的降低。强烈表明，在攻击性雄性蟋蟀中，自生鸣声影响大脑功能，例如中央体神经元中 5-HT 的合成和/或降解，导致突触传递的改变。