Identification of fish larvae collected from Tokyo Bay based on genetic biology

基于遗传生物学的东京湾鱼类幼体鉴定

• 批准号: 17580161
• 负责人: KOHNO Hiroshi
• 金额: $ 1.86万
• 依托单位: Tokyo University of Marine Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580161/
• 关键词: larvae and juveniles; taxonomy; Tokyo Bay; gene biology; morphology; Yellowfin goby; Acanthogobius

Larvae of two gobiids, Acanthogobius flavimanus and A. lactipes, distributed and abundant in the inner part of Tokyo Bay, are difficult to be identified by their morphological characters ; however, they are distinguished each other by their occurrence seasons, the former occurring in Spring and the latter in Summer. Therefore, in this study, larvae of both the species were tried to be identified by the genetic characters.Adult fish and larvae/juveniles were collected from the inner part of Tokyo Bay. The adults were identified by their morphological characters. Furthermore, their cytochrome b gene of mitochondrial DNA, amplified by using the polymerase chain reaction (PCR), was analyzed by RFLP method, and the nucleotide sequences were obtained.The larvae were, on the other hand, divided into two types, Type A occurred in Spring and Type B in Summer, and the same genetic analyses were done as in the adults. Consequently, 99.9% of nucleotide sequences of these larvae were matched with those of A. flavimanus and A. lactipes, respectively; therefore, Type A and B larvae were identified to be the respective species. Detailed study on morphology, especially melanophore distribution of these larvae, revealed that the distribution patterns of melanophores on the isthmus and lateral part of body were different between these two species.The methods performed in this study such as larval collection, larval identification by genetic characters and detailed morphological studies of larvae can be applied widely to the field of larval taxonomy.

在东京湾内部分布且丰富的两个Gobiids，Acanthogobius flavimanus和A. lactipes的幼虫很难被其形态特征鉴定出来。但是，他们的出现季节彼此区分，前者发生在春季，后者在夏季。因此，在这项研究中，试图通过遗传特征鉴定这两个物种的幼虫。从东京湾的内部收集了亚种鱼和幼虫/幼虫。成年人通过形态学特征来识别。此外，通过使用聚合酶链反应（PCR）扩增了线粒体DNA的细胞色素B基因，通过RFLP方法进行了分析，并获得了核苷酸序列。另一方面，幼虫在春季和夏季和分析中均在夏季和分析中分为A型A类型A型出现在春季和B中。因此，这些幼虫的核苷酸序列的99.9％分别与黄曲霉和乳酸曲霉的核苷酸序列相匹配。因此，A型和B幼虫被确定为各自的物种。关于形态学的详细研究，尤其是这些幼虫的黑色素酚分布，表明这两个物种之间的黑色素载体的分布模式与身体的分布模式不同。本研究中执行的方法，例如幼虫收集，通过遗传特征鉴定幼体特征和幼虫的详细形态研究，可以广泛地应用于larval a raLval agalson的领域。