Feeding behavior and growth in Chicks treated RNA interference

经 RNA 干扰处理的小鸡的摄食行为和生长

• 批准号: 17580237
• 负责人: BUNGO Takashi
• 金额: $ 2.3万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580237/
• 关键词: Chicks; Feeding behavior; RNA interference; Peptide hormone; Central nervous system

(1) Effects of RNAi in vitro: We found that RNAi treatments temporarily inhibited the expression of chLEPR mRNA and the protein in COS-7 cells. In CHO-chLEPR cell strains, the luciferase activity of RNAi treated group was significantly lower than that of control at 3, but not 24 hr after leptin loading (125 ng/ml). It seemed that the designed RNAi was effective to inhibition of the signaling transmission in the leptin receptors.(2) Effects of RNAi in vivo: The designed RNAi did not affect the expression of chLEPR mRNA in diencephalon of chicks at 15 or 24 hr after injection. Similar to the expression, the injection did not influence on both feed intake and body weight gain in chicks.(3) Effect of anti-sense oligonucleotide for leptin or NPY Y1 receptor (in vivo) : Similar to RNAi, anti-sense oligonucleotide for leptin receptor did not affect the expression of chLEPR mRNA, and feed intake and body weight gain in chicks. On the other hand, anti-sense oligonucleotide for NPY Y1 receptor suppressed the expression of the receptor mRNA at 15 hr after injection.

(1)RNAi体外效应：我们发现RNAi处理暂时抑制了COS-7细胞中chLEPR mRNA和蛋白的表达。在CHO-chLEPR细胞株中，在加载瘦素(125ng/ml)后3小时，但不是24小时，RNAi处理组的荧光素酶活性显着低于对照。设计的RNAi似乎能够有效抑制瘦素受体的信号传导。(2)RNAi的体内效应：注射后15或24小时，设计的RNAi不影响雏鸡间脑chLEPR mRNA的表达。与表达相似，注射对雏鸡的采食量和体重增加均没有影响。 (3)瘦素或NPY Y1受体反义寡核苷酸的影响(体内)：与RNAi类似，瘦素受体反义寡核苷酸不影响chLEPR mRNA的表达，以及雏鸡的采食量和体重增加。另一方面，注射后15小时，NPY Y1受体的反义寡核苷酸抑制了受体mRNA的表达。