Effects of transdermal absorption of 1,25(OH)_2D_3 to Hypocalcaemia in Cows.

1,25(OH)_2D_3 透皮吸收对奶牛低钙血症的影响。

• 批准号: 17580277
• 负责人: NAITO Yoshihisa
• 金额: $ 2.18万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580277/
• 关键词: cows; hypocalcaemia; 1,25(OH)_2D_3; calcium metabolism; post-parturient disease; milk fever; transdermal; prevention; 周産期疾患

In this studies, we did vaginal or transdermal absorption of 1,25(OH)_2D_3 for prevention of hypocalcaemia in dairy cows.1.Vaginal absorption of 1,25(OH)_2D_3 in cattleIn the first study, the 1,25(OH)_2D_3 was administered into the bovine vaginal lumen, subsequently to observe the blood biochemical changes in the 1,25(OH)_2D_3 and some minerals. The plasma 1,25(OH)_2D_3 levels reached to a peak at 6 hr. The plasma Ca increased from 6 hr, and reached at 24 hr in the peak. The plasma iP increased at 6 hr, and reached to the plateu during 24 to 96hr.2.Transdermal absorption of 1,25(OH)_2D_3 in cattle.In the second study, the 1,25(OH)_2D_3 was dropped into the ventral root of tail of Jersey cattle, subsequently to observe the blood biochemical changes in the 1,25(OH)_2D_3 and some minerals. The plasma 1,25(OH)_2D_3 of increased at 2 hr after dropping. The plasma Ca at skin dropping test was higher from 6 hr. The plasma iP at skin dropping test was higher from 24 to 120 hr.In conclusion, these results indicates the possibility of the intravaginal or transdermal dropping 1,25(OH)_2D_3 for prophylaxis of parturient hypocalcaemia in cows.

在本研究中，我们通过阴道或透皮吸收1,25(OH)_2D_3来预防奶牛低钙血症。牛阴道对1,25(OH)_2D_3的吸收在第一项研究中，将1,25(OH)_2D_3注入牛阴道腔，随后观察血液中1,25(OH)_2D_3和一些矿物质的生化变化。血浆1,25(OH)_2D_3水平在6小时达到峰值。血钙从6小时开始升高，24小时达到峰值。血浆iP在6小时升高，24 ~ 96小时达到平稳。牛对1,25(OH)_2D_3的透皮吸收。在第二项研究中，将1,25(OH)_2D_3滴入泽西牛的尾腹根部，随后观察1,25(OH)_2D_3和一些矿物质的血液生化变化。血浆1,25(OH)_2D_3在下降后2小时升高。血浆钙降皮试验从6小时开始升高。24 ~ 120小时降皮试验血浆iP较高。综上所述，阴道内滴入或经皮滴入1,25(OH)_2D_3可能对奶牛产后低钙血症有预防作用。

项目成果

乳牛の低Ca血症予防のための基礎試験 : 1,25(OH)_2D_3の経皮吸収

预防奶牛低钙血症的基本测试：1,25(OH)_2D_3的透皮吸收

• 发表时间: 2007
• 期刊: 日本家畜臨床学会誌 (In press)
• 作者: 浪岡 徹;山岸則夫;大倉徳太;内藤善久
• 通讯作者: 内藤善久

The expression of genes for transepithelial calcium-transporting protein in the bovine duodeum

跨上皮钙转运蛋白基因在牛十二指肠中的表达

• 发表时间: 2006
• 期刊: Veterinary Journal 171
• 作者: N.Yamagishi;M.Miyazaki;Y.Naito
• 通讯作者: Y.Naito

Dose Response to Vaginal Administration of 1,25(OH)_2D_3 to cows

奶牛阴道给药 1,25(OH)_2D_3 的剂量反应

• 发表时间: 2006
• 期刊: Veterinary Journal (In press)
• 作者: N.Okura;N.Yamagishi;Y.Naito;M.Koiwa
• 通讯作者: M.Koiwa

若齢牛における1, 25-dihydroxyvitamin D_3 の経皮吸収

1, 25-二羟基维生素D_3在幼牛体内的真皮吸收

• 期刊: 日本家畜臨床学会誌 (印刷中)
• 作者: N.Yamagishi;M.Miyazaki;Y.Naito;波岡 徹 他
• 通讯作者: 波岡 徹 他

Dose response to vaginal administration of 1, 25-dihydroxyvitamin D_3 to cows

奶牛阴道给药 1, 25-二羟基维生素 D_3 的剂量反应

• 发表时间: 2006
• 期刊: Veterinary Journal
• 影响因子: 2.2
• 作者: Norimoto Okura;et al.
• 通讯作者: et al.