Immunohistochemical observations on the initial disorders of the epiphyseal growth plate in the cause of Hyena disease.

鬣狗病病因中骨骺生长板初始紊乱的免疫组织化学观察。

• 批准号: 11836003
• 负责人: NAITO Yoshihisa
• 金额: $ 2.11万
• 依托单位: IWATE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11836003/
• 关键词: vitamin A; Bovine Hyena disease; epiphyseal growth plate; rat; collagen; osteoclast; calcification; chondrocite matrix; 骨端軟骨板; レチノイン酸

Bovine Hyena disease is regarded as a disorder of skeletal growth, with an appearance similar to a Hyena figure due to lower hind limbs than fore limbs in height. It has been definitely shown by us that Hyena disease is caused by excessive administration of vitamin A (VA). In the present study, as the next step, we examined the succeeding histological disorders in the process of the focal disappearance of the epiphyseal growth plate cartilage histochemically in young rats administered a high dose of VA.1. Animals were given 100,000 IU/100 g body weight/day of VA for 5 days from 4 weeks after birth and sacrificed on Day 12 and 19 of the experiment. Tibiae were examined by immunohistochemistry for type I, II and X collagens, and lectin-histochemistry for Helix pomatia. These findings suggested that the cartilage matrix of the epiphyseal growth plate was abnormally calcified and showed the phenotypes like bone matrix. The eroded regions of the epiphyseal growth plated seemed to be caused by the invasion of osteoclasts into the altered cartilage matrix and might develop to the focal disappearances by the modeling or remodeling due to action of osteoclasts and osteioblasts.2. A differential process from the proliferatic chondrocites to the hypertrofic chondrocites was examined in a retinoic acid (RA) of metabolic products of VA with the centrifugal culture methods. It was shown that decreases of the matrix of proliferatic chondrocites were occurred by addition of RA.These findings indicated that a modeling or remodeling process, such as the absorption of the calcified cartilage matrix by osteoclasts and the formation of bone tissues by osteoblasts, were occurred within the epihyseal growth plate in VA rats. Such a modeling or remodeling might further proceed, and finally bring about the focal disappearances of the epiphyseal growth plate.

牛鬣狗病被认为是一种骨骼生长障碍，由于后肢的高度低于前肢，其外观类似于鬣狗。我们已经明确表明，鬣狗病是由过量服用维生素A（VA）引起的。在本研究中，作为下一步，我们研究了随后的组织学疾病的过程中的局灶性消失的骺生长板软骨组织化学在年轻大鼠给予高剂量的VA。1。动物从出生后4周开始接受100，000 IU/100 g体重/天VA给药，持续5天，并在实验第12天和第19天处死。用免疫组化法检测I型、II型和X型胶原，用凝集素组织化学法检测苹果酸酯。这些结果表明，骨骺生长板的软骨基质异常钙化，并表现出类似骨基质的表型。骨骺生长板的侵蚀区域可能是由于破骨细胞侵入改变的软骨基质所致，并可能在破骨细胞和成骨细胞的作用下通过造模或重塑发展至局灶性消失.用离心培养法研究了VA代谢产物维甲酸（RA）中增殖软骨细胞向肥大软骨细胞的分化过程。结果表明，RA可使增殖软骨基质减少，提示VA大鼠骺生长板内存在破骨细胞吸收钙化软骨基质、成骨细胞形成骨组织等造模或重塑过程。这种塑形或重塑可能进一步进行，最终导致骺生长板的局灶性消失。

项目成果

Soeta,S.,and Naito,Y., et al.: "Histological desorders related to the focal desappearence of the epiphyseal growth plate in rats induced by high dose of vitamin A."J.Vet.Med.Sci.. 62. 293-299 (2000)

Soeta, S. 和 Naito, Y. 等人：“与高剂量维生素 A 诱导的大鼠骨骺生长板局灶性消失相关的组织学紊乱。”J.Vet.Med.Sci. 62。