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Clinico-Pharmacological Studies of 1,25(OH)_2D_3 to Hypocalcemia in Cows.

1,25(OH)_2D_3 治疗奶牛低钙血症的临床药理学研究。

基本信息

批准号：
13660313
负责人：
NAITO Yoshihisa
金额：
$ 2.3万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660313/
关键词：
cows hypocalcemia 1,25(OH)_2D_3 calcium metabolism post-parturient disease milk fever TRAP 骨代謝 TRAP 予防 1,25(OH)_2D_3

项目摘要

1. In the first study, 1,25-dihydroxyvitamin D_3 [1,25(OH)_2D_3] was injected into late-pregnant cows 12-48 hours prepartum to evaluate the effect of 1,25(OH)_2D_3 for prevention of parturient hypocalcemia. Twelve Holstein cows, at least second parity, were assigned to two groups. Seven cows received 1 μg/kg of 1,25(OH)_2D_3 (1,25(OH)_2D_3 group) in ethanol, and the other five received only ethanol (control group). All cows calved 12-48 hours after administration. Plasma 1,25(OH)_2D_3 concentration in 1,25(OH)_2D_3 group was significantly higher 1 day pre- to 0.5 day postpartum than in control group. Plasma Ca and iP levels in 1,25(OH)_2D_3 group kept high near parturition, and were significantly higher than those in control group from 0.5 day before parturition to 2 days after parturition.In conclusion, it was considered that the intramuscular injection of 1,25(OH)2D3 before 1-2 day(s) of calving had prophylactic effect for parturient hypocalcemia.2. The aim of the second study was to investigate association between occurrence of milk fever and bone metabolism. To observe changes in bone metabolism and associations between it and the development of milk fever, we investigated serum tartrate-resistant acid phosphatase (TRAP) activity at each lactating stage n 83 cows.In this study, it was confirmed utilization of Trap activity in serum for evaluation of bone metabolism in cow. Occurrence of milk fever thought to be related with inactive bone metabolism from the finding of low TRAP activity in milk fever frequent farm. It was thought that measuring of serum TRAP activity was applied to prevent milk fever.

1.第一项研究是在妊娠晚期母牛产后12-48小时注射1，25-二羟维生素D_3 [1，25（OH）_2D_3]，以评价1，25（OH）_2D_3预防临产低钙血症的效果。12头荷斯坦奶牛，至少第二次产次，被分配到两组。1，25（OH）_2D_3组（7头）注射1，25（OH）_2D_3乙醇溶液（1 μg/kg），对照组（5头）注射乙醇溶液。所有奶牛在给药后12-48小时产犊。1，25（OH）_2D_3组产后1天至产后0.5天血浆1，25（OH）_2D_3浓度显著高于对照组。1，25（OH）_2D_3组临产前血浆Ca和iP水平保持较高水平，从临产前0.5天到产后2天均显著高于对照组，因此认为产犊前1-2天肌肉注射1，25（OH）_2D_3对产妇低钙血症有预防作用。第二项研究的目的是调查产奶热的发生与骨代谢之间的关系。为观察奶牛骨代谢的变化及其与产奶热发生的关系，测定了83头奶牛不同泌乳期血清抗酒石酸酸性磷酸酶（TRAP）活性，证实了TRAP活性可用于奶牛骨代谢的评价。从在奶热多发农场中发现低TRAP活性，认为奶热的发生与骨代谢不活跃有关。认为测定血清TRAP活性可用于预防乳热。