Analysis of the bacterial flora for the zero emission oriented toilet system and improvement of the clarification function

实验室规模的模型零排放厕所系统一直使用人类排泄物，直到2005年。2006年，由于人类排泄物的成分每天都在变化，厕所系统使用了由蛋白调、酵母膏、纤维素和氯化钠组成的人工污水来代替人类排泄物。木片或海绵块被包装在处理槽中作为细菌载体。结果表明，木屑和海绵对BOD的去除效果分别为83%和67%。木片需要补充，因为木片被细菌消化了。在海绵格子池中观察到水流方向的变化，可以认为这是BOD去除较少的原因。2006年，将处理池改进为充填海绵格子的好氧池和厌氧池两级。该系统对BOD的去除效率几乎超过90%。分别对好氧池和厌氧池的菌群进行了分析。细菌区系和BOD的测定结果表明，类杆菌门中的细菌对好氧池中BOD的去除起主要作用，类杆菌门和贝塔保护杆菌门中的细菌对厌氧池中的BOD去除起主要作用，我将通过FISH法和定量PCR来分析细菌数量的变化对好氧池中BOD去除的影响。

The laboratory-scale model zero emission oriented toilet system was operated with the human excrement until 2005. In 2006, the toilet system was operated with the artificial sewage which consisted of peptone, yeast extract, cellulose and NaCl instead of the human excrement, because the components of the human excrement daily changed. And the wood chips or the sponge cubes were packed in a treatment tank as bacterial supports. The results were that the removal of BOD in the wood chips and sponge cubes was 83 and 67%, respectively. The wood chips were necessary to replenish, since the chips were digested by bacteria. The changing the direction of the flow tract was observed in the sponge cubes tank, so it could be considered the reason of less BOD removal.In 2006, the treatment tank was improved to two levels of aerobic tank and anaerobic tank that filled up sponge cubes. The BOD removal rates in this system almost exceeded 90%. The bacterial flora of the aerobic and anaerobic tank was analyzed, respectively. The results of the bacterial flora and the measurement of the BOD were that the bacteria in the phylum Bacteroidetes carried a main role of the BOD removal in the aerobic tank, and that the bacteria in the phylum Bacterioidetes and the class Betaproteobacteria carried a main role of the BOD removal in the anaerobic tank.I am going to perform FISH method and quantitive PCR to analyze a change of bacterial quantity to affect the BOD removal suggested by this study in future.