Development of microchip liquid chromatography for ultra-sensitive analysis of trace amounts of bio-molecules.

开发用于微量生物分子超灵敏分析的微芯片液相色谱。

• 批准号: 17590030
• 负责人: SANTA Tomofumi
• 金额: $ 2.24万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590030/
• 关键词: chromatography; microchip; fluorescence; mass spectrometry; labeling reagent; benzofurazan; LC; MS

The purpose of this study is development of microchip liquid chromatography for ultra-sensitive analysis of trace amounts of bio-molecules. At first, water-soluble fluorescent derivatization regents having benzofurazan structure were developed. These reagents are expected to prevent the adsorption of the analytes to the analytical instruments and improve the sensitivity. Next, fluorescent bi-cyclic nucleic acid, deoxycitidine derivative was synthesized. This structure might be useful as the fluorescent derivatization reagents. Using the synthesized reagents and high-performance liquid chromatography, we have developed the sensitive determination methods of bio-molecules and drugs. These methods are applicable for the microanalysis using microchip liquid chromatography. Mass spectrometry(MS) is promising for the detection method of microchip liquid chromatography. Thus, the derivatization reagents for MS were developed and applied to the analysis of bio-molecules such as fatty acids in biological samples. These methods are also applicable for the microchip liquid chromatography. For the small scale separation, we have synthesized monolithic column in capillary column and on microchip. Finally, we have tried to develop the fluorescence detection system by laser excitation. The compounds near the object lens are sensitively detectable. Therefore, the narrow space less than 5 μm was prepared in the flow area of the microchip and used for the laser excitation fluorescence detection. This method enabled the sensitive detection of the fluorescent compounds. In the future, the methods developed in this study, the derivatization reagents, the micro separation monolithic columns and the fluorescent micro detection system, will be summarized in the microanalysis using microchip liquid chromatography.

本研究旨在发展微芯片液相层析技术，以超灵敏度分析微量生物分子。首先，开发了具有苯并呋咱结构的水溶性荧光衍生试剂。这些试剂有望防止分析物吸附到分析仪器上，提高灵敏度。其次，合成了具有荧光的双环核酸脱氧胞苷衍生物。该结构可用作荧光衍生试剂。利用合成的试剂和高效液相色谱技术，建立了生物分子和药物的灵敏检测方法。这些方法适用于微芯片液相色谱的微量分析。质谱法是一种很有前途的微芯片液相色谱检测方法。因此，开发了MS衍生试剂并应用于生物样品中脂肪酸等生物分子的分析。这些方法也适用于微芯片液相色谱。对于小规模分离，我们在毛细管柱和微芯片上合成了整体柱。最后，我们尝试研制了激光激发荧光检测系统。靠近物镜透镜的化合物是可以灵敏地检测到的。因此，在芯片的流动区域制备了小于5 μm的狭窄空间，用于激光激发荧光检测。该方法能够灵敏地检测荧光化合物。今后将对本研究开发的衍生化试剂、微分离整体柱和荧光微检测系统在微芯片液相色谱微量分析中的应用进行总结。