Development of the prominent labeling reagents for LC/MS/MS and sensitive analysis of the biomolecules

开发用于 LC/MS/MS 和生物分子灵敏分析的著名标记试剂

• 批准号: 21590039
• 负责人: SANTA Tomofumi
• 金额: $ 3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590039/
• 关键词: 分析化学; 標識試薬; クロマトグラフィー; 質量分析; LC/MS/MS; 生体分子; 新生児マススクリーニング

Liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) is widely used method. In this research, several labeling reagents were synthesized to enhance the sensitivity and selectivity in LC/MS/MS. These reagents enabled sensitive analysis of the biomolecules such as diseases markers. These methods were useful for the screening of the inherited metabolic disorders.

液-质联用(LC/MS/MS)是目前广泛使用的一种分析方法。本研究合成了几种标记试剂，以提高LC/MS/MS的灵敏度和选择性。这些试剂可以对疾病标志物等生物分子进行灵敏分析。这些方法可用于遗传性代谢紊乱的筛查。

项目成果

Novel approaching for the determination of methycitrate in dried blood spots (DBS) by liquid chromatography tandem mass spectrometry.

液相色谱串联质谱法测定干血斑 (DBS) 中甲基柠檬酸盐的新方法。

• 发表时间: 2011
• 作者: Al-Dirbashi OY;McIntosh N;McRoberts C;Rashed MS;Geraghty MT;Fisher L;Santa T;Chakraborty P.
• 通讯作者: Chakraborty P.

Analysis of3-hydroxyglutalic acid in dried urine spots by LC-MS/MS; application for the diagnosis of glutaric aciduria type 1.

采用 LC-MS/MS 分析干燥尿斑中的 3-羟基戊二酸；

• 发表时间: 2011
• 作者: Al-Dirbashi OY;Kolker S;Ng D;Fisher L;Rupar T;Lepage N;Rashed MS;Santa T;Goodman SI;Geraghty MT;Zschocke J;Christensen E;Hoffmann GE;Chakraborty P.
• 通讯作者: Chakraborty P.

Novel approach for quantification of fatty acid markers of peroxisomal disorders by LC-MS/MS.

通过 LC-MS/MS 定量过氧化物酶体疾病脂肪酸标记物的新方法。

• 发表时间: 2009
• 作者: Al-Dirbashi;Osama Y
• 通讯作者: Osama Y

Isothiocyanates as derivatization reagents for amines in liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS)

异硫氰酸盐作为液相色谱/电喷雾电离串联质谱 (LC/ESI-MS/MS) 中胺的衍生试剂

• 发表时间: 2010
• 期刊: Biomedical Chromatography
• 影响因子: 1.8
• 作者: Kohei Otomo;Akira Toyama;Takashi Miura;Hideo Takeuchi;Santa T
• 通讯作者: Santa T

Zellweger Syndrome Caused by PEX13 Deficiency: Report of Two Novel Mutations

• DOI: 10.1002/ajmg.a.32874
• 发表时间: 2009-06-01
• 期刊: AMERICAN JOURNAL OF MEDICAL GENETICS PART A
• 影响因子: 2
• 作者: Al-Dirbashi, O. Y.;Shaheen, R.;Alkuraya, F. S.
• 通讯作者: Alkuraya, F. S.