Quantitative analysis of mRNA of Intraductal papillary mucinous neoplasms: To differentiate carcinoma from benign neoplasms

导管内乳头状粘液性肿瘤 mRNA 的定量分析：区分癌与良性肿瘤

• 批准号: 17591412
• 负责人: NAGAI Eishi
• 金额: $ 2.24万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591412/
• 关键词: gene; pancreatic cancer; diagnosis; 癌; 臨床

Intraductal papillary mucinous neoplasms(IPMN) are common cystic tumors of the pancreas and are known to show transition from adenoma to carcinoma accompanied by several molecular abnormalities. The prognosis of patients with intraductal papillary mucinous carcinoma is poor. It is very important to make a early diagnosis of carcinoma to reduce the mortality of the patients. We used quantitative real-time reverse transcription-PCR with gene specific priming to measure MUC1, MUC4, MUC5AC, MUC6, SlOOP, S100A11, Sonic hedghog(SHH), hTERT in various types of pancreatic tumours.The quantitative analyses of MUC1, MUC5AC, S 100P, S100A11 and SHH are useful for differentiation between neoplasms and non-neoplastic lesions. There is significant differences in the expression of hTERT between carcinoma and IPMN. The hTERT analysis of pancreatic juice may provide some advantages for the follow-up of a subset of patients with IPMN.

导管内乳头状粘液性肿瘤是胰腺常见的囊性肿瘤，可由腺瘤转变为癌，并伴有多种分子异常。导管内乳头状黏液腺癌预后差。早期诊断对降低患者死亡率具有重要意义。应用实时定量逆转录聚合酶链反应（real-time reverse transcription-PCR，RT-PCR）结合基因特异性引物，检测了不同类型胰腺肿瘤组织中MUC 1、MUC 4、MUC 5AC、MUC 6、S100 P、S100 A11、S10 hTERT在肿瘤和IPMN中的表达存在显著差异。胰液的hTERT分析可能为IPMN患者亚组的随访提供一些优势。

项目成果

SlOOP is early Developmental marker of pancreatic carcinogenesis

SlOOP是胰腺癌发生的早期发展标志物

• 发表时间: 2006
• 期刊: Clin Cancer Res 12(8)
• 作者: Ohuchida K;Nagai E et al.
• 通讯作者: Nagai E et al.

S100A11, a putative tumor suppressor gene, is overexpressed in pancreatic carcinogenesis

• DOI: 10.1158/1078-0432.ccr-06-0222
• 发表时间: 2006-09-15
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Ohuchida, Kenoki;Mizumoto, Kazuhiro;Tanaka, Masao
• 通讯作者: Tanaka, Masao

Sonic hedgehog is an early development marker of intraductal papillary mucinous neoplasms : clinical implications of mRNA levels in pancreatic juice

Sonic Hedgehog是导管内乳头状粘液性肿瘤的早期发展标志物：胰液中mRNA水平的临床意义

• 发表时间: 2006
• 期刊: J Pathol 210
• 作者: Ohuchida K;Nagai E et al.
• 通讯作者: Nagai E et al.

Quantative analysis of MUC1 and MUC5AC mRNA in pancreatic juice for preoperative diagnosis of Pancreatic cancer

胰液中MUC1和MUC5AC mRNA的定量分析用于胰腺癌术前诊断

• 发表时间: 2006
• 期刊: Int. J. Cancer 118
• 作者: Ohuchida K;Nagai E et al.
• 通讯作者: Nagai E et al.

Sl00A11, A putative tumor suppressor gene, is overexpressed in pancreatic carcinogenesis

Sl00A11，一种假定的肿瘤抑制基因，在胰腺癌发生过程中过度表达

• 发表时间: 2006
• 期刊: Clin Cancer Res 12(8)
• 作者: Ohuchida K;Nagai E et al.
• 通讯作者: Nagai E et al.