Continuous significant effects of intravenous administration of lipo-prostaglandin El on the ischemic gastric tube in pigs

脂前列腺素El持续静脉注射对猪胃管缺血的显着效果

• 批准号: 17591438
• 负责人: IKEDA Yoshifumi
• 金额: $ 2.11万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591438/
• 关键词: esophageal cancer; anastomosis; leakage; tissue blood flow; vasodilator; PGE1; lipo-PGE1; 胃管; Layser-Doppler flowmetry法; PGE1濃度

Introduction: Lipo-prostaglandin E1 (PGE1) is a new preparation of PGE1 in which it is bound to lipids in order to slow PGE1 release and delay its rate of metabolism. We investigated the change of the blood flow on impaired microcirculation of the ischemic gastric tube in pigs after saline, unmodified PGE1 and lipo-PGE1 administration.Materials and Methods: The gastric tube was constructed using fifteen domestic pigs under general anesthesia, and saline, unmodified PGE1 and lipo-PGE1 were infused continuously at a rate of 0.05μg/kg/min for 10 minutes. Tissue blood flow (TBF) were analyzed before administration, after administration for 10 minutes, and until 120 minutes after the end of administration.Results: There were no obvious changes in TBF during the administration of saline. However, TBF during treatment with unmodified PGE1 and lipo-PGE1 was significantly increased to 13.1 ± 1.3 and 13.5 ± 1.4 ml/min/100g, respectively (paired t-test; p<0.01). Although TBF was significantly decreased to 8.0 ± 1.0 ml/min/100g on 10 minutes after the end of unmodified PGE1 administration (paired t-test; p<0.01), it was maintained over 10 ml/min/100g until 120 minutes in lipo-PGE1 group.Conclusion: Lipo-PGE1 infusion leads to the objectively measurable improvement and the prolonged action in the blood perfusion of the gastric tube in pigs. Therefore, treatment with lipo-PGE1 infusion can increase TBF at the anastomotic site, and it may be beneficial for preventing anastomotic leakage in boluses for a couple of times a day.

前言：前列腺素E_1脂微球是一种新型的前列腺素E_1制剂，它与脂类结合，以减缓前列腺素E_1的释放，延缓其代谢速度。材料与方法：15头家猪在全身麻醉下建立胃管，以0.05min0.05g/kg/μ的速度持续滴注生理盐水、未修饰前列腺素E_1和前列腺素E_1脂微循环10分钟。分别于给药前、给药后10min、给药结束后120min测定组织血流量。结果：生理盐水给药过程中组织血流量无明显变化。而未修饰前列腺素E_1和脂微球前列腺素E_1治疗期间的总血流量分别为13.1±1.3和13.5±1.4ml/min/100g(配对t检验，p&lt；0.01)。在给药后10min，未修饰PGE1组的总胆红素流量显著下降至8.0±1.0ml/min/100g(配对t检验，p&lt；0.01)，但脂微球前列腺素E_1组的血流量一直维持在10ml/m in/100g以上，直到120分钟。结论：脂微球前列腺素E_1输注对猪胃管血液灌流有客观可测量的改善和延长作用。因此，输注前列腺素E_1脂微球可以增加吻合口的血流量，而且每天几次可能有利于预防吻合口瘘。