Continuous significant effects of intravenous administration of lipo-prostaglandin El on the ischemic gastric tube in pigs

简介：脂质体前列腺素E1（Lipo-prostaglandin E1，PGE 1）是一种新型的前列腺素E1制剂，它与脂质结合，以减缓PGE 1的释放和延缓其代谢速率。材料与方法：15头家猪在全麻下建立胃管，分别以生理盐水、未修饰的PGE_1和脂质体PGE_1（lipo-PGE_1），以0.05μg/kg/min的速度持续灌注10 min，观察生理盐水、未修饰的PGE_1和脂质体PGE_1对缺血胃管微循环的影响。分别于给药前、给药后10 min及给药结束后120 min测定组织血流量（TBF）。然而，在用未修饰的PGE 1和脂质体-PGE 1治疗期间，TBF分别显著增加至13.1 ± 1.3和13.5 ± 1.4ml/min/100 g（配对t检验; p<0.01）。TBF在未修饰的PGE_1给药结束后10分钟显著下降至8.0 ± 1.0 ml/min/100 g（配对t检验; p<0.01），在lipo-PGE 1组中维持超过10 ml/min/100 g直到120分钟。Lipo-PGE 1输注导致猪胃管血液灌注的客观可测量的改善和延长的作用。因此，用脂质体-PGE 1输注治疗可以增加吻合口部位的TBF，并且每天几次大剂量输注可能有利于预防吻合口瘘。

Introduction: Lipo-prostaglandin E1 (PGE1) is a new preparation of PGE1 in which it is bound to lipids in order to slow PGE1 release and delay its rate of metabolism. We investigated the change of the blood flow on impaired microcirculation of the ischemic gastric tube in pigs after saline, unmodified PGE1 and lipo-PGE1 administration.Materials and Methods: The gastric tube was constructed using fifteen domestic pigs under general anesthesia, and saline, unmodified PGE1 and lipo-PGE1 were infused continuously at a rate of 0.05μg/kg/min for 10 minutes. Tissue blood flow (TBF) were analyzed before administration, after administration for 10 minutes, and until 120 minutes after the end of administration.Results: There were no obvious changes in TBF during the administration of saline. However, TBF during treatment with unmodified PGE1 and lipo-PGE1 was significantly increased to 13.1 ± 1.3 and 13.5 ± 1.4 ml/min/100g, respectively (paired t-test; p<0.01). Although TBF was significantly decreased to 8.0 ± 1.0 ml/min/100g on 10 minutes after the end of unmodified PGE1 administration (paired t-test; p<0.01), it was maintained over 10 ml/min/100g until 120 minutes in lipo-PGE1 group.Conclusion: Lipo-PGE1 infusion leads to the objectively measurable improvement and the prolonged action in the blood perfusion of the gastric tube in pigs. Therefore, treatment with lipo-PGE1 infusion can increase TBF at the anastomotic site, and it may be beneficial for preventing anastomotic leakage in boluses for a couple of times a day.