In Vivo Evaluation of Small Caliber Vascular Prosthesis Impregnated with Bone Marrow Cells Overexpressing eNOS.

使用腺病毒介导的基因转导系统，我们已经创建了间充质干细胞（MSC）过度表达eNOS。从大鼠心脏cDNA文库中分离eNOS cDNA，并将其亚克隆到腺病毒中。从大鼠骨髓中制备MSC，用重组腺病毒转染。为了检测eNOS基因转导的MSCs在体内的有益作用，我们将转导的MSCs植入皮下注射野百合碱诱导的肺动脉高压大鼠体内。通过监测右室收缩压、右室肥厚和生存时间来评价其有益效果。本研究将转eNOS基因的MSCs植入小口径人工血管，以期在天然冠状动脉床上提供可行的NO和MSCs促血管生成作用，并改善自身通畅性。我们计划使用动物模型来评估过表达eNOS的人造血管的有用性。

Using an adenovirus-mediated gene transduction system, we have created mesenchymal stem cells (MSCs) overexpressing eNOS. The eNOS cDNA was isolated from a rat heart cDNA library and subcloned into adenovirus. The MSCs were prepared from rat bone marrow and transfected with the recombinant adenovirus. The production of eNOS in the MSCs was confirmed enzymatically.In order to examine the beneficial effect of the eNOS gene-transduced MSCs in vivo, we implanted the transduced MSCs into rats suffering from pulmonary hypertension which was induced by subcutaneous injection of monocrotaline. The beneficial effect was evaluated by monitoring right ventricular systolic pressure, right ventricular hypertrophy and survival time. The implantation of the eNOS gene-transduced MSCs was clearly effective in the treatment of the pulmonary hypertension.Then, we impregnated the eNOS gene-transduced MSCs into small caliber vascular prostheses which are expected to offer feasible effects of NO and angiogenic effects of MSCs on the native coronary arterial beds, as well as improvement of self-patency. We are planning to evaluate the usefulness of the synthetic vascular prostheses overexpressing eNOS using animal model.