Basic research to sonodynamic therapy for the brain tumor

脑肿瘤声动力治疗的基础研究

• 批准号: 11671407
• 负责人: FUKUSHIMA Takeo
• 金额: $ 0.38万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671407/
• 关键词: rat brain; C6glioma cell; human glioma cell; ultrasound; sonochemical agent; blood brain barrier; sonodynamic therapy; Photofrin; C6グリオーマ細胞; ヒトグリオーマ細胞

In this study, we investigated whether or not the sonodynamic treatment might induced tissue damage in the rat normal brain, and the effect of sonodynamic treatment for glioma cells in vitro. In the normal brain tissue of rats, focused ultrasound irradiation with or without Rose bengal (RB) induced localized destructive lesions, and the occurrence of lesions was proportional to the intensity of the ultrasound and the irradiation time. The lesions were induced with a cavitation effect as well as a heat effect, because no difference was observed in the brain temperature between irradiation with and without RB.The lesions investigated by Evans blue histochemically became larger with RB than without RB suggesting an opening of the blood brain barrier due to sonochemical activity. Apoptosis was induced in C6 rat glioma cells, and two human glioma cell lines (U251 and U105) by ultrasound with photosensitizers (Photofrin, sonodynamic treatment), which was strongly inhibited by rat recombinant RAP.The effect of ultrasound with photosensitizers (Photofrin) in human glioma cells depended on the intensity and duration of the ultrasound treatment. These results indicated that a sonodynamic therapy can be used in the brain if the exposure parameters were selected properly, and it might be effective for the treatment of glioma.

本研究旨在探讨声动力治疗是否会对大鼠正常脑组织造成损伤，以及声动力治疗对体外培养的胶质瘤细胞的影响。在大鼠正常脑组织中，聚焦超声辐照加或不加虎红（RB）均引起局部破坏性损伤，损伤的发生与超声强度和辐照时间成正比。的病变引起的空化效应，以及热效应，因为没有观察到的差异，在照射与不RB之间的脑温度。伊文思蓝组织化学研究的病变变得更大，RB比没有RB表明开放的血脑屏障，由于声化学活性。超声联合光敏剂（Photofrin，声动力学处理）可诱导C6大鼠胶质瘤细胞和两种人胶质瘤细胞系（U251和U105）凋亡，而大鼠重组RAP可强烈抑制该作用。超声联合光敏剂（Photofrin，声动力学处理）对人胶质瘤细胞的作用依赖于超声处理的强度和时间。这些结果表明，声动力学治疗可以用于脑内，如果选择适当的曝光参数，它可能是有效的治疗胶质瘤。

项目成果

野中将,福島武雄: "先端医療シリーズ6脳神経外科脳神経外科の最先端No.2第1章脳腫瘍治療への挑戦4.音響化学療法"先端医療技術研究所. 472 (2000)

野中正志、福岛武夫：“先进医学系列第 6 期神经外科第 2 期神经外科前沿第 1 章对脑肿瘤治疗的挑战 4. 声学化疗”先进医疗技术研究所 472 (2000)。

野中将,福島武雄: "先端医療シリーズ6・脳神経外科 脳神経外科の最先端No.2第1章 脳腫瘍治療への挑戦 4音響化学療法"先端医療技術研究所. 472 (2000)

野中正志、福岛武夫：“先进医学系列6：神经外科，神经外科最前沿的第2号，第1章：脑肿瘤治疗的挑战4：声学化疗”，先进医疗技术研究所472（2000）。