Basic research to sonodynamic therapy for the brain tumor

脑肿瘤声动力治疗的基础研究

• 批准号: 09671458
• 负责人: FUKUSHIMA Takeo
• 金额: $ 1.6万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671458/
• 关键词: Sonodynamic therapy; Brain tumor; Rat; Ultrasound; Rose bengal; ultrasound; sonodynamic approach; 活性化学物質; Rose Bengal; Cavitation効果

A sonodynamic approach has been proposed for the treatment of brain tumors. Prior to clinical application, investigations were made to clarify whether or not the sonodynamic approach might induce tissue damage in the normal brain and demonstrate how effective it was on experimental brain tumor in rats. 1) In the normal brain tissue of rats, focused ultrasound irradiation with or without Rosebengal induced localized destructive lesions and the occurrence or lesions was proportional to the intensity of the ultrasound and the irradiation time. 2) The lesions which coincidentally occurred in the area irradiated with focused ultrasound, was discrete when focusing on the basal ganglia and coagulation necrosis was observed in the histological examination. After Rosebengal was administered, the lesions became larger and associated with focal hemorrhage. 3) The mechanism of tissue damage was considered to mainly be due to the heat effect relation to ultrasound irradiaton, because the brain temp … More erature increased remarkably during ultrasound irradiation. Following ultrasound irradiation, the lesions became larger with Rosebengal administration than without Rosebengal, and thus the lesions were induced with a cavitation effect as well as a heat effect, because no difference was observed in the brain temperature between irradiation with and without Rosebengal. 4) The lesions enhanced by Evans blue became larger with Rosebengal than without Rosebengal, thus suggesting an opening of the blood brain barrier due to sonochemical activity. 5) The intensity of ultrasound, which induced destructive lesions, was of 100 w/cm^2 for 5 minutes and the lesion was the same after 1 hour and 24 hours. With an irradiation intensity of 24 w/cm^2 for 5 minutes, none of the destructive or demyelinating lesions were found 2 months later. 6) In terms of the sonochemical effect to brain tumors in rats, the tumors turned into either a fusiform or a crescent after forcused irradiation, thus suggesting a reduction in the tumor which was more significant after Rosebengal administration. These results indicated that sonodynamic therapy was thus effective for the treatment of brain tumors in rats. Sonodynamic therapy is one of newly developed treatment modalities for brain tumors and it might be also applicable to residual tumors by means of intraoperative sonodynamic irradiation. Less

已经提出了声动力方法用于治疗脑肿瘤。在临床应用之前，进行了研究以澄清声动力方法是否会引起正常大脑的组织损伤，并证明它对大鼠实验性脑肿瘤的有效性。 1）在大鼠正常脑组织中，使用或不使用Rosebengal的聚焦超声照射可引起局部破坏性病变，且病变的发生与超声强度和照射时间成正比。 2)聚焦超声照射部位同时发生的病变，聚焦基底节时病变离散，组织学检查可见凝固性坏死。服用 Rosebengal 后，病灶变大并伴有局灶性出血。 3）组织损伤的机制被认为主要是与超声照射的热效应有关，因为超声照射时脑温度显着升高。超声照射后，给予 Rosebengal 的病灶比未给予 Rosebengal 的病灶更大，因此通过空化效应和热效应诱导病灶，因为在照射和未照射 Rosebengal 之间没有观察到脑温度差异。 4) 使用 Rosebengal 时，伊文思蓝增强的病灶比未使用 Rosebengal 时更大，因此表明血脑屏障由于声化学活性而打开。 5)引起破坏性损伤的超声强度为100w/cm^2，持续5分钟，1小时和24小时后损伤相同。照射强度24w/cm^2，照射5分钟，2个月后未发现任何破坏性或脱髓鞘病变。 6) 就声化学对大鼠脑肿瘤的影响而言，在强制照射后，肿瘤变成梭形或新月形，这表明肿瘤缩小，并且在给予Rosebengal后更显着。这些结果表明声动力疗法对于治疗大鼠脑肿瘤是有效的。声动力疗法是新开发的脑肿瘤治疗方式之一，也可能适用于术中声动力照射的残留肿瘤。较少的