Bone tissue engineering by transplantation of culture expanded bone marrow cells and platelet rich plasma

1.骨特异性碱性磷酸酶（BAP），羧基末端前肽的I型胶原（P1CP），和骨钙素的分泌和mRNA表达的升高，在培养的细胞与成骨补充剂和不。在体内骨形成，这是量化的面积的百分比，是阳性钙化（%钙化）在扩散室，与活性的BAP和P1CP。骨钙素活性与钙化百分比无相关性。2.检测骨髓细胞在不同血小板浓度PRP凝胶中的成骨分化和增殖能力。PRP凝胶的增殖能力与血小板浓度呈正相关，而骨髓细胞的成骨分化能力在凝胶间无显著性差异。3.将GFP（绿色荧光蛋白）转基因大鼠骨髓细胞移植到大鼠延长模型延长终止后的牵张区，以验证移植细胞的定位。移植后21天的组织学检查显示GFP标记的细胞定位于新形成的松质骨内。4.回顾性比较细胞移植与未移植骨在牵张成骨中的作用，以评估该细胞疗法的疗效。治疗组的平均愈合指数明显低于未治疗组。治疗组的并发症发生率也显著降低。股骨延长组的愈合速度明显快于胫骨细胞移植延长组。

1.The secretion and mRNA expression of bone-specific alkaline phosphatase (BAP), carboxy-terminal propeptide of type I collagen (P1CP), and osteocalcin were elevated in the cells cultured with and without osteogenic supplements. In vivo bone formation, which was quantified by the percentage of area that was positive for calcification (% calcification) in the diffusion chamber, was correlated with the activity of BAP and P1CP. There was no correlation between the osteocalcin activity and the % calcification.2.Osteogenic differentiation and proliferation capability were examined among the bone marrow cells cultured within the PRP gel with different platelet concentration. Positive correlation was demonstrated between the proliferation capability and the platelet concentration of PRP gel, whereas, there were no significant differences in osteogenic differentiation of bone marrow cells among the gels.3.The cells harvested from GFP (green fluorescent protein) transgenic rats were transplanted into the distracted area of the rat lengthening model after the termination of lengthening to verify the localization of the transplanted cells. Histological examinations of the 21 days after transplantation showed that the GFP labeled cells were localized within the newly formed trabecular bone.4.Retrospective comparative study was conducted between the bones treated with and without cell transplantation in distraction osteogenesis to assess the efficacy of this cell therapy. The average healing index of the treated group was significantly lower than that of the untreated group. The rate of complications was also significantly lower in the treated group. The femoral lengthening showed significantly faster healing than the tibial lengthening by the cell transplantation.