Osteogenic differentiation of culture expanded bone marrow cells under tensile and compressive strains

拉伸和压缩应变下培养扩增的骨髓细胞的成骨分化

• 批准号: 21591915
• 负责人: KITOH Hiroshi
• 金额: $ 2.83万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591915/
• 关键词: メカニカルストレス; 骨髄細胞培養; 骨組織再生; 骨延長モデル; 多血小板血漿; HIF 1α; 三次元培養; 細胞培養; 骨形成マーカー; 骨髄間葉系細胞; 骨芽細胞分化; VEGF

Alkaline phosphatase activity within the culture media were significantly elevated in the dynamic stretch(DS) group and the dynamic compression(DC) group compared to the control group. The DC group showed significant elevated levels of mRNA expression of HIF 1α. Western blotting analysis demonstrated increased amount of HIF 1αprotein within the nucleous in the DC, DS, and static stretch(SS) groups. Favorable new bone formation was observed when the bone marrow cells cultured under the dynamic stretch condition were implanted with PRP into the distraction gap of rat bone lengthening models.

与对照组相比，动态拉伸（DS）组和动态压缩（DC）组培养基中碱性磷酸酶活性显著升高。DC组HIF 1α mRNA表达水平显著升高。Western blotting分析显示，在DC、DS和静态拉伸（SS）组，细胞核内HIF 1α蛋白的数量增加。动态拉伸条件下培养的骨髓细胞经PRP植入大鼠骨延长模型牵张间隙后，新骨形成良好。

项目成果

Novel and recurrent TRPV4 mutations and their association with distinct phenotypes within the TRPV4 dysplasia family

• DOI: 10.1136/jmg.2009.075358
• 发表时间: 2010-10-01
• 期刊: JOURNAL OF MEDICAL GENETICS
• 影响因子: 4
• 作者: Dai, J.;Kim, O-H;Ikegawa, S.
• 通讯作者: Ikegawa, S.

ペルテス病の治療成績に関連する因子の検討

Perthes病治疗结果相关因素的研究

• 发表时间: 2011
• 作者: 鬼頭浩史;金子浩史;北小路隆彦;服部義;石黒直樹.
• 通讯作者: 石黒直樹.

Hyperuricemia cosegregating with osteogenesis imperfecta is associated with a mutation in GPATCH8

• DOI: 10.1007/s00439-011-1006-9
• 发表时间: 2011-11-01
• 期刊: HUMAN GENETICS
• 影响因子: 5.3
• 作者: Kaneko, Hiroshi;Kitoh, Hiroshi;Ohno, Kinji
• 通讯作者: Ohno, Kinji

Drug screening for transcriptional activation of SOX9

SOX9转录激活药物筛选

• 发表时间: 2011
• 作者: Kaneko H;Kitoh H;Yamamoto R;IshiguroN;Ohno K
• 通讯作者: Ohno K

Induction of systemic bone changes by preconditioning total body irradiation for bone marrow transplantation

骨髓移植预处理全身照射诱导全身骨变化

• 发表时间: 2009
• 期刊: Pediatr Radiol 39
• 作者: Miyazaki O;Nishimura G;Okamoto R;Masaki H;Kumagai M;Shioda Y;Nozawa K;Kitoh H
• 通讯作者: Kitoh H