Study of placental formation and functional differentiation by tissue specific stem cell transplantation

组织特异性干细胞移植研究胎盘形成和功能分化

• 批准号: 17591728
• 负责人: SUZUKI Ayako
• 金额: $ 2.24万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591728/
• 关键词: IUGR; placenta; mononuclear cell; glucocorticoid; fetal growth; 妊娠; 胎児; 分岐鎖アミノ酸; IUGR

The placenta plays an essential role in the growth of fetus in the uterus. Any insult in placental development, such as abnormal immune reaction or maternal malnutrition, can result in poor placental growth and placental insufficiency. In the present study, we evaluated an involvement of maternal bone-marrow derived mononuclear cells in the development of the placenta by transplanting these cells in growing placenta. Bone-marrow cells obtained from GFP transgenic mice were injected in the growing placenta and was observed to be retained in the placental tissue just prior to delivery. Moreover, GFP positive maternal mononuclear cells were observed in the placenta of non-GFP pups growing in the uterus of GFP transgenic dams, thus indicating that maternal mononuclear cells may contribute somehow to the formation of the placenta. In the present study, we also elucidated an involvement of glucocorticoid inactivating enzyme, 11 beta-hydroxysteroid dehydrogenase type 2(11βHSD2). 11βHSD2 was expressed in chorionic epithelium of the mouse placenta. 11βHSD2 expression in the placenta was increased by maternal food restriction during pregnancy. Therefore, it is implicated that fetus is protected from exposure to glucocorticoid in maternal circulation increased by food restriction stress. In addition, placental 11βHSD2 expression was further increased by feeding high protein diet during food restriction, together with improved fetal growth. These findings may give us a clue to develop a new strategy to have a good fetal outcome.

胎盘对胎儿在子宫内的生长发育起着至关重要的作用。任何对胎盘发育的损害，如免疫反应异常或母体营养不良，均可导致胎盘生长不良和胎盘功能不全。在本研究中，我们通过将母体骨髓来源的单核细胞移植到生长中的胎盘中，评估了这些细胞在胎盘发育中的作用。从GFP转基因小鼠获得的骨髓细胞被注射到生长中的胎盘中，观察到在分娩前胎盘组织中保留了细胞。此外，在GFP转基因母鼠子宫内生长的非GFP幼崽的胎盘中也观察到GFP阳性的母核细胞，这表明母核细胞可能在某种程度上参与了胎盘的形成。在本研究中，我们还阐明了糖皮质激素失活酶11β -羟基类固醇脱氢酶2型（11β hsd2）的参与。11βHSD2在小鼠胎盘绒毛膜上皮中表达。11βHSD2在胎盘中的表达随着孕期母体食物限制而增加。因此，胎儿被保护免受暴露于糖皮质激素在母体循环增加食物限制应激。此外，在限食期间饲喂高蛋白饲料可进一步提高胎盘11βHSD2的表达，促进胎儿生长。这些发现可能给我们提供了一个线索，以开发一种新的策略，以获得良好的胎儿结局。

项目成果

A case of spontaneous regression of congenital cystic adenomatoid malformation of the lung -Longitudinal examinations by magnetic resonance imaging

先天性肺囊性腺瘤样畸形自然消退一例——磁共振纵向检查

• 发表时间: 2005
• 期刊: Congenital Anomalies 45・4
• 作者: 鈴木彩子;他;鈴木彩子;Kazuyo Kakui;Makoto Kawamura
• 通讯作者: Makoto Kawamura

Diagnostic criteria based on magnetic resonance imaging for uterine sarcoma

子宫肉瘤的磁共振诊断标准

• 发表时间: 2005
• 期刊: Acta Obst Gynaec Jpn Vol.57
• 作者: 鈴木彩子;他;鈴木彩子;Kazuyo Kakui;Makoto Kawamura;鈴木彩子;鈴木彩子;Kazuyo Kakui;Makoto Kawamura;Ayako Suzuki;Ayako Suzuki
• 通讯作者: Ayako Suzuki

IN VIVO ANTI-TUMOR EFFECT OF DUAL RELEASE OF CISPLATIN AND ADRIAMYCIN FROM BIODEGRADABLE GELATIN HYDROGEL

• DOI: 10.1136/ijgc-00009577-200409001-00695
• 发表时间: 2004-09
• 期刊: International Journal of Gynecologic Cancer
• 作者: Masatoshi Kariya;Yasuhiko Tabata;Mitsunaga Konishi;H. Yagi;Shingo Fujii

Experimental Transplantation Study for Possible Transformation of Bone Marrow Cells in the Mouse Placenta

小鼠胎盘骨髓细胞可能转化的实验移植研究

• 发表时间: 2005
• 期刊: Placenta 26・8-9
• 作者: 鈴木彩子;他;鈴木彩子;Kazuyo Kakui
• 通讯作者: Kazuyo Kakui

非定型子宮筋腫(グレーゾーン)

非典型子宫肌瘤（灰色区域）

• 发表时间: 2005
• 期刊: 日本産科婦人科学会雑誌 57
• 作者: T.Yasuda;H.Takeshita;T.Nakajima;K.Mogi;Y.Kaneko;R.Iida K.Kishi;鈴木 彩子
• 通讯作者: 鈴木 彩子