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Transplantation of bone marrow stromal cells via aorta for rat spinal ischemia model

经主动脉移植骨髓基质细胞建立大鼠脊髓缺血模型

基本信息

批准号：
17591875
负责人：
TOKUMINE Joho
金额：
$ 2.05万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591875/
关键词：
bone marrow stromal cell transplantation spinal ischemia regeneration 経動脈神経再生再生医学

项目摘要

Some reports have suggested that bone marrow stromal cells may differentiate into neurons. We hypothesized that bone marrow stromal cells transplanted in the spinal cord after ischemic injury may differentiate into neurons and promote the recovery of motor function.In previous studies, bone marrow stromal cells were transplanted directly through surgery. The results of these studies were unsatisfactory because the transplanted cells differentiated into glia rather than neurons. The reason for this was thought to be the effect of inflammation of the local spinal tissue caused by surgical damage. We aimed to employ a non-injurious technique, unlike the conventional method, for transplantation by using the arterial flow to the spinal cord tissue.A rat spinal cord ischemia model was prepared by transient aortic occlusion and reperfusion using intra-aortic balloon under low systemic blood pressure. A rat suffering from motor dysfunction of the lower extremities over 2 weeks was used in the … More transplantation experiment. The bone marrow stromal cells were transplanted via the aorta by inserting a catheter from the femoral artery.Histochemistry revealed that many bone marrow stromal cells had been transplanted to the spinal cord tissue. Some of the transplanted cells migrated from the blood vessel inside the interstitial tissue and were positive for a specific neuronal maker. However, most transplanted cells did not migrate to the interstitial tissue and remained in the blood vessel. Our evaluation of motor function in these rats showed that all rats that underwent transplantation did not recover.In this study, many bone marrow stromal cells could be transplanted without any damage to the spinal cord tissue. In addition, some of the transplanted cells migrated to the ischemic-injured interstitial tissue of the spinal cord and expressed a specific neuronal marker.Further studies are expected to reveal the chemotactic factor that directs the migration of marrow stem cells to the ischemic-injured interstitial tissue of the spinal cord. Less

有报道认为骨髓基质细胞可以分化为神经元。我们假设骨髓基质干细胞移植于缺血损伤后的脊髓内可能分化为神经元，促进运动功能的恢复，在以往的研究中，骨髓基质干细胞是通过手术直接移植的。这些研究的结果并不令人满意，因为移植的细胞分化成神经胶质而不是神经元。其原因被认为是手术损伤引起的局部脊柱组织炎症的影响。本研究采用动脉阻断脊髓组织的方法建立大鼠脊髓缺血模型，并在低体压条件下采用主动脉内球囊阻断脊髓组织，建立脊髓缺血模型。将患有下肢运动功能障碍超过2周的大鼠用于实验。 ...更多信息移植实验从股动脉插入导管经主动脉移植骨髓基质细胞，组织化学显示大量骨髓基质细胞已移植到脊髓组织中。一些移植的细胞从血管迁移到间质组织内，并对特定的神经元标记呈阳性。然而，大多数移植的细胞没有迁移到间质组织，而是留在血管中。我们对这些大鼠运动功能的评估显示，所有接受移植的大鼠都没有恢复。在这项研究中，许多骨髓基质细胞可以在不损伤脊髓组织的情况下移植。此外，部分移植细胞迁移至脊髓缺血损伤间质并表达特异性神经元标志物，进一步研究有望揭示指导骨髓干细胞迁移至脊髓缺血损伤间质的趋化因子。少