Analysis on the optimum site for harvest site in tissue engineering of the human ear shaped cartilate

人耳形软骨组织工程最佳采集位点分析

• 批准号: 17591881
• 负责人: ISOGAI Noritaka
• 金额: $ 2.3万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591881/
• 关键词: tissue engineering; chondrocyte; mechanical test; メカニカルテスト

In this experiment, we cultured chondrocytes that were harvested chondrocytes from 4 different sites (nasoseptal, articular, costal and auricular cartilage), which were subsequently seeded onto biodegradable poly(LA-CL,50:50) polymer. Chondrocyte-copolymer composites were implanted in vivo, and then analyzed for long term observation that includes gross morphology, histology, and mechanical property. Results on gross morphology revealed that all tissue engineered cartilages retained human ear shape for up to 40 weeks in vivo. Such tissue engineered cartilages as nasoseptal, articular, and auricular origin demonstrated similar mechanical property with the native bovine auricular cartilage. On the contrary, tissue engineered cartilage of costal origin developed mineralization and showed remarkably rigid mechanical property, which is indicative of probable stem cells in cartilage structure. These results lead us to conclude that tissue engineered cartilages of nasoseptal, articular, and auricular origin were appropriate sites as cell sources as they retained three dimensional shape of the human ear with the restoration of original mechanical property, while that of costal origin further needed to regulate the mineralization process.

在该实验中，我们培养了从4个不同部位（鼻中隔、关节、肋和耳软骨）收获的软骨细胞，随后将其接种到可生物降解的聚（LA-CL，50：50）聚合物上。将软骨细胞-共聚物复合物植入体内，然后进行长期观察，包括大体形态学、组织学和机械性能。大体形态学结果显示，所有组织工程软骨在体内保持人耳形状长达40周。这些组织工程软骨如鼻中隔软骨、关节软骨和耳廓软骨表现出与天然牛耳廓软骨相似的力学性能。相反，肋缘来源的组织工程化软骨形成矿化，并显示出显著的刚性机械性能，这表明软骨结构中可能存在干细胞。这些结果使我们得出结论，组织工程软骨的鼻中隔，关节和耳廓起源的合适的网站作为细胞来源，因为他们保留了三维形状的人耳与原始的机械性能的恢复，而肋起源的进一步需要调节矿化过程。

项目成果

Comparison of different chondrocytes for use in tissue engineering of cartilage model structares

不同软骨细胞用于软骨模型结构组织工程的比较

• 发表时间: 2006
• 期刊: Tissue Engineeing Apr;12 (4)
• 作者: Isogai N;Kusuhara H;Ikada Y;Ohtani H;etc
• 通讯作者: etc

Comparison of different chondrocytes for use in tissue engineering of cartilage model structures

• DOI: 10.1089/ten.2006.12.691
• 发表时间: 2006-04-01
• 期刊: TISSUE ENGINEERING
• 作者: Isogai, Noritaka;Kusuhara, Hirohisa;Landis, William J.
• 通讯作者: Landis, William J.