Effect of local environment, fibrin, and basic FGF incorporation on a canine autologous model of bio-engineered cartilage tissue

局部环境、纤维蛋白和基本 FGF 掺入对生物工程软骨组织的犬自体模型的影响

• 批准号: 21592300
• 负责人: ISOGAI Noritaka
• 金额: $ 3.08万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592300/
• 关键词: 生分解性高分子; 自家移植; 軟骨再生; bFGF; 徐放システム; フィブリン; 生分解性ポリマー; 軟骨; 組織工学; ポリマー

We developed a technique to form a bioabsorbable synthetic polymer(poly glycolic acid ; PGA) combined with a natural polymer(fibrin) to serve as a scaffold to help retain seeded cells and improve the seeding efficiency of chondrocytes in an implantable construct. This approach was evaluated in a canine autologous implant model of bio-engineered cartilage. The implantation site(subcutaneous or intra-fascial) and the use of basic fibroblast growth factor(b-FGF) were also evaluated with this system. The intra-fascial implantation site yielded optimal results, especially when used in conjunction with fibrin and a b-FGF sustained-release system incorporated into the complex. A thicker, more sustained cartilagenous layer was formed, with a more vascularized outer fibrous supporting tissue layer. This combined approach of implant environment selection, natural polymer for cell retention, and growth factor supplementation offers a more optimized method for generating bio-engineered auricular cartilage.

我们开发了一种技术，形成一种生物可吸收的合成聚合物（聚乙醇酸; PGA）与天然聚合物（纤维蛋白）相结合，作为一种支架，以帮助保留接种的细胞，并提高软骨细胞在可植入结构中的接种效率。在犬自体生物工程软骨植入模型中评价了这种方法。还使用该系统评价了植入部位（皮下或筋膜内）和碱性成纤维细胞生长因子（b-FGF）的使用。筋膜内植入部位产生了最佳效果，特别是当与纤维蛋白和b-FGF缓释系统结合使用时。形成了更厚、更持久的软骨层，具有更血管化的外部纤维支持组织层。这种植入环境选择、用于细胞保留的天然聚合物和生长因子补充的组合方法为产生生物工程化耳软骨提供了更优化的方法。

项目成果

Cytokine-Rich autologous serum system for cartilaginous tissue engineering in the shape of human ear

用于人耳形状软骨组织工程的富含细胞因子的自体血清系统

• 发表时间: 2009
• 作者: Mohammad Ghazizadeh;Hajime Shimizu;Isogai N.
• 通讯作者: Isogai N.

Advances in tissue engineering models of human phalanx and ears

人类指骨和耳朵组织工程模型的进展

• 发表时间: 2008
• 作者: Sato M.;Yajima Y.;Ogura K.;Shimizu M.;Nishitho Y.;Miyachi H.;植村貞繁;Isogai N
• 通讯作者: Isogai N

Tissue engineering of the ear : Effect of local environment, fibrin, and basie FGF incorporation on a canine autologous model

耳组织工程：局部环境、纤维蛋白和基础 FGF 掺入对犬自体模型的影响

• 发表时间: 2011
• 期刊: Oral & Craniofacila Tissue Engineering
• 作者: Sato S;Ghazizadeh M;Isogai N
• 通讯作者: Isogai N

Tissue engineering of the ear : Effect of local environment, fibrin, and basic FGF incorporation on a canine autologous model

耳组织工程：局部环境、纤维蛋白和基本 FGF 掺入对犬自体模型的影响

• 发表时间: 2012
• 作者: Wakabayashi T;Kosaka J;Mochii M;Miki M;Mori T;Takamori Y and Yamada H;Isogai N
• 通讯作者: Isogai N

Topical application of b-FGF-impregnated gelatin microspheres to tissue engineer bone and cartilage or plastic surgical reconstruction

b-FGF 浸渍明胶微球在组织工程骨和软骨或整形外科重建中的局部应用

• 发表时间: 2011
• 作者: 丸藤哲;澤村淳;早川峰司;菅野正寛上垣慎二;久保田信彦;Isogai N
• 通讯作者: Isogai N