权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Free Radical Monitoring of Brain Injury and their Basic Studies

脑损伤的自由基监测及其基础研究

基本信息

批准号：
17591895
负责人：
SATOH Kazue
金额：
$ 2.05万
依托单位：
SHOWA University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591895/
关键词：
electron spin resonance (ESR)free radical alkoxyl radical brain injury reactive oxygen species spin trapping lipid hydroperoxide oxidative stress アルコキシルラジカル

项目摘要

Oxygen Free radicals have important roles of various acute neuronal conditions. Especially neuroemergency diseases such as neurotrauma, stroke, traumatic brain injury and ischemia after cardiac arrest are strongly related with oxidative stress. However, the study of free radicals in body fluid is currently severely hampered by technical difficulties in their detection. We have developed an ex vivo electron spin resonance (ESR) method, that employs 5,5-dimethyl-l-pyrroline-N-oxide (DMPO) as a spin trap, to measure the alkoxyl radical (^●OR) in human blood. We found that this method can detect ^●OR produced by lipid peroxidation, which is caused by reactive oxygen species. Blood samples were collected from the catheter of the internal jugular bulb. The control samples were also collected from healthy volunteers. Alkoxyl radical level of all patients with neuroemergency deseases, who were admitted to Showa University Hospital Emergency Centre, were elevated in comparison with control. Alkoxyl radical level was shown the condition of patients and was suppressed after administration of Edaravone, a novel synthetic free radical scavenger shown to exert neuroprotective effect in acute ischemic stroke. This method is useful to monitor the free radical level immediately at bedside and it will enable to detect the oxidative stress and the severity of brain damage. In the future we will examine to prevention and the deletion using antioxidant or antioxidative food. This study was supported by ethical committee of Showa University.

氧自由基在各种急性神经元疾病中具有重要作用。特别是神经创伤、脑卒中、创伤性脑损伤、心脏骤停后缺血等神经急症与氧化应激密切相关。然而，体液中自由基的研究目前受到检测技术困难的严重阻碍。我们建立了一种以5，5-二甲基-1-吡咯啉-N-氧化物（DMPO）为自旋捕集剂的离体电子自旋共振（ESR）方法，用于测定人血液中的烷氧基自由基（^●OR）。我们发现这种方法可以检测由活性氧引起的脂质过氧化反应产生的^●OR。从颈内静脉球导管采集血样。还从健康志愿者中采集对照样品。在昭和大学医院急救中心的神经急症患者中，烷氧基自由基水平与对照组相比均升高。烷氧基自由基水平显示了患者的状况，并在给予依达拉奉后受到抑制，依达拉奉是一种新型的合成自由基清除剂，在急性缺血性卒中中发挥神经保护作用。该方法可用于在床边立即监测自由基水平，并能够检测氧化应激和脑损伤的严重程度。今后我们将研究使用抗氧化剂或抗氧化食品来预防和消除。本研究得到昭和大学伦理委员会的支持。

项目成果

期刊论文数量（69）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Cytotoxic Activity of Styrylchromones against Human Tumor Cell Lines.

苯乙烯色酮对人肿瘤细胞系的细胞毒活性。

DOI：
发表时间：
2005
期刊：
In Vivo 19
影响因子：
0
作者：
Momoi K.;Sugita Y.;Ishihara M.;Satoh K.;Kikuchi H.;Hashimoto K.;Yokoe I.;Nishikawa H.;Fujisawa S.;Sakagami H.
通讯作者：
Sakagami H.

Protection by Antioxidants of Copper-induced Decline of Proliferation and SOD Activity.

抗氧化剂对铜引起的增殖和 SOD 活性下降的保护。

DOI：
发表时间：
2005
期刊：
Anticancer Res. 25
影响因子：
0
作者：
Kinoshita N.;Hashimoto K.;Yamamura T.;Teramura H.;Koizumi T.;Satoh K.;Katayama T.;Sakagami H.
通讯作者：
Sakagami H.

Influence of SNPs in cytokine-related genes on the severity of food allergy and atopic eczema in children

DOI：
10.1111/j.1399-3038.2006.00463.x
发表时间：
2006-12-01
期刊：
PEDIATRIC ALLERGY AND IMMUNOLOGY
影响因子：
4.4
作者：
Negoro, Takaharu;Orihara, Kanami;Tobe, Takashi
通讯作者：
Tobe, Takashi

Investigation of False-positive Reactions for CBH351 Maize in Screening PCR Analysis;

筛选 PCR 分析中 CBH351 玉米假阳性反应的调查；

DOI：
发表时间：
2006
期刊：
J.Food Hyg. Soc Japan 47
影响因子：
0
作者：
Monma K;Moriuchi R;Sagi N;Ichikwa H;Satoh K;Tobe T;Kamata K
通讯作者：
Kamata K

Elevated Levels of Bilirubin in Patients withNeurotrauma Reflect its Pathophysiological Role in Free Radical Scavenging

神经创伤患者胆红素水平升高反映了其在自由基清除中的病理生理作用

DOI：
发表时间：
2005
期刊：
In Vivo 19
影响因子：
0
作者：
Sakagami H;Matsumoto H;Satoh K;Shioda S;Chowdhury SA;Hashimoto K;Kikuchi H;Nishikawa H;Terakubo S;Shoji Y;Nakashima H;Shimada J;Momoi K et al.;Kinoshita N et al.;Suzuki F. et al.;Dohi K et al.
通讯作者：
Dohi K et al.