Pathways and fate of epithelial root sheath cells during cementogenesis in rat incisors

大鼠门牙牙骨质形成过程中上皮根鞘细胞的途径和命运

• 批准号: 17591903
• 负责人: KAWANO Yoshiro
• 金额: $ 2.24万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591903/
• 关键词: epithelio-mesenchymal transformation; S100; cementblasts; heat shock protein; 器官培養; 歯根上皮鞘

It is well known that the epithelial root sheath is greatly involved in the root dentin formation and the development of periodontium. Two possibilities on the fate of epithelial root sheath cells are proposed, the pathway to be extinguished by the apoptosis, and the pathway to transform into cementblasts by epithelio-mesenchymal transformation. Phenotypic transformation from the epithelial cell into the mesenchymal cell is reported in developing Muellerian duct and the epithelial cells in the median palatine suture, and recognized in the transformation from cancer cells into invasive metastatic cells in a pathogenic case.The origin of cementblastic cells remains controversial. Mature cementblasts produce matrix proteins common to osteoblasts, such as Type I collagen, osteocalcin, and osteopontin. These features led to the suggestion that the cementblasts are uniquely positioned osteoblasts. On the other hand, morphological data and the expression in some cementblasts of dental epithel … More ium markers, such as karatin, E-cadherin and amelin/ameloblastion/sheathlin, support the hypothesis that root epithelium-derived cells also persist during cementum formation. These findings support the epithelial-mesenchymal transformation hypothesis that epithelial root sheath cells differentiate into cementoblasts. However, to date, the spatial-temporal behavior of epithelial root sheath cells and its mechanism has not been elucidated.The immunohistochemisty demonstrated that a certain kind of heat shock protein was expressed continuously from the epithelial cells to the cementblasts on the root dentin and that a certain kind of aquapolin and S100 protein were expressed in the cementoblasts in the rat incisor. The expression pattern of these proteins during cementum formation illustrates the complex origin of cementblasts and the fate of epithelial root sheath cells. These results suggest mesenchymal transition in odontogenic epithelium and the importance of these proteins during the cement genesis. Less

众所周知，上皮根鞘在牙根牙本质形成和牙周组织发育中起重要作用。上皮根鞘细胞的命运提出了两种可能性，即通过细胞凋亡而被消灭的途径和通过上皮间质转化而转化为成牙骨质细胞的途径。在Muellerian管和腭中缝上皮细胞的发育过程中，上皮细胞向间充质细胞的表型转化已被报道，并在一个致病病例中，癌细胞向侵袭性转移细胞的转化也被确认。成熟的成骨细胞产生成骨细胞常见的基质蛋白，如I型胶原、骨钙素和骨桥蛋白。这些特征提示成骨细胞是唯一定位的成骨细胞。另一方面，我们还对牙本质细胞的形态学数据和在牙本质细胞中的表达进行了研究。 ...更多信息 牙本质标记物，如karatin、E-cadherin和amelin/ameloblastion/sheathlin，支持这样的假设，即根上皮衍生的细胞在牙骨质形成期间也持续存在。这些发现支持上皮-间充质转化假说，即上皮根鞘细胞分化为成牙骨质细胞。然而，迄今为止，上皮根鞘细胞的时空行为及其机制尚未阐明，细胞化学研究表明，在大鼠切牙中，从上皮细胞到成牙骨质细胞，某种热休克蛋白持续表达，成牙骨质细胞中表达某种水蛋白和S100蛋白。这些蛋白质在牙骨质形成过程中的表达模式说明了成牙骨质细胞的复杂起源和上皮根鞘细胞的命运。这些结果提示牙源性上皮的间充质转化以及这些蛋白在牙骨质形成过程中的重要性。少

项目成果

Characteristic distribution of immunoreaction for estrogen receptor alpha (ERα) in rat ameloblasts

大鼠成釉细胞雌激素受体α（ERα）免疫反应的特征分布

• 发表时间: 2005
• 期刊: Anat.Rec. 284A
• 作者: Simamura;E.;Ferrer V-L et al.;Ferrer V-L et al.
• 通讯作者: Ferrer V-L et al.