Excretion of cytoplasmic proteins (ECP) in Staphylococcus (A06)

许多微生物和真核生物分泌典型的细胞质蛋白。由于没有一个经典的分泌系统参与，这种类型的分泌被称为“非经典蛋白质分泌”。假设ECP是由细胞溶解引起的。然而，越来越多的证据表明，细胞溶解不是ECP的主要原因。我们可以证明，在金黄色葡萄球菌ECP发生在指数生长期，像Sec系统易位蛋白。我们计划鉴定与ECP相关的基因，并希望解决ECP的机制。

Many microorganisms and also eukaryotes excrete typical cytoplasmic proteins. As none of the classical secretion systems appears to be involved, this type of secretion has been referred to as "nonclassical protein secretion". It is assumed that ECP is caused by cell lysis. There are however mounting evidences that cell lysis is not the primary cause of ECP. We could show that in Staphylococcus aureus ECP occurs in the exponential growth phase, like Sec-system translocated proteins. We plan the identification of genes involved in ECP and would like to solve the mechanism of ECP.