Flowcytometer for high dimensional analytics (64 channels) and multiwell uptake

流式细胞仪技术已经成为伊萨尔克里尼库姆大学比德斯坦校区的一匹主力马。除了血液分析，以前被带入单细胞悬液中的组织细胞也越来越多地被分析。免疫学研究中的限制因素仍然是人类细胞材料的有限获取，因此可用颜色的数量对比德斯坦校区的所有研究人员来说极其重要。因此，现在可能扩展到最高颜色是真正向前迈出的根本一步。即将到来的一个重要挑战是研究从组织中分离出来的细胞，将其引入单细胞悬液中，并使用表面标记进行流式细胞术表征。此外，基于分泌产物的功能参数允许得出功能结论。组织细胞和组织的先天免疫细胞(呼吸道、痰、皮肤活检、肠道活检)多种多样(树突状细胞、先天淋巴细胞、γ-T细胞)。呼吸道和皮肤屏障的基础干细胞分析对少量细胞的分子分化提出了很高的要求。此外，上皮细胞中有很强的自发荧光，现在可以很容易地管理甚至用于细胞鉴定。现有的设备也是当时通过DFG大规模设备应用获得的--这种设备对校园的必要性是毋庸置疑的。不幸的是，旧设备(在使用了近10年后)不再可靠地工作，导致巨大的维护成本，激光也在使用寿命的尽头。然而，所要求的设备(除了较低的运营成本和旧设备的更新换代)也是一个重要的创新元素，因为它消除了技术限制，开辟了新的研究可能性，并最终将促进使用有限细胞材料的研究，从而为比德斯坦校区的临床研究带来新的推动力。

Flow cytometric technology has become a "main workhorse" on the Biederstein campus of the Klinikum rechts der Isar. In addition to blood analyzes, cells from tissue that have previously been brought into single-cell suspension are also increasingly being analyzed. The limiting factor in immunological research is and will remain the limited access to human cell material and therefore the number of available colors is extremely important for all researchers at the Biederstein campus. The now possible expansion to up to 64 colors is therefore really a fundamental step forward. An important upcoming challenge is the research of cells that are isolated from the tissue, brought into single cell suspension and characterized with flow cytometry using surface markers. Furthermore functional parameter based on secreted products allow functional conclusions. Tissue cells and innate immune cells of the tissue (brushings of the respiratory tract, sputum, skin biopsies, intestinal biopsies) are diverse (dendritic cells, innate lymphocytes, gamma delta-T cells). Basal stem cell analysis of airway and skin barrier place high demands on molecular differentiation with a small number of cells. In addition, there is strong autofluorescence in epithelial cells that can now be easily managed and even utilised for cell identification.The existing device was also acquired at the time through a DFG large-scale device application - the necessity of this equipment for the campus was beyond question. Unfortunately, the old device (after almost 10 years of use) no longer works reliably, causes immense maintenance costs and the lasers are also at the end of their life. However, the requested device is (in addition to the lower operating costs and replacement of the old one) also an important innovation element, as it removes technical limitations, opens up new research possibilities and will ultimately facilitate research with limited cell material and thus new impulses for clinical research at the Biederstein campus will put.