Flowcytometer for high dimensional analytics (64 channels) and multiwell uptake

用于高维分析（64 通道）和多孔吸收的流式细胞仪

• 批准号: 491149881
• 金额: --
• 依托单位: Technische Universität München (TUM)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/491149881?language=en
• 关键词: Flowcytometer; dimensional; analytics; 64; channels

Flow cytometric technology has become a "main workhorse" on the Biederstein campus of the Klinikum rechts der Isar. In addition to blood analyzes, cells from tissue that have previously been brought into single-cell suspension are also increasingly being analyzed. The limiting factor in immunological research is and will remain the limited access to human cell material and therefore the number of available colors is extremely important for all researchers at the Biederstein campus. The now possible expansion to up to 64 colors is therefore really a fundamental step forward. An important upcoming challenge is the research of cells that are isolated from the tissue, brought into single cell suspension and characterized with flow cytometry using surface markers. Furthermore functional parameter based on secreted products allow functional conclusions. Tissue cells and innate immune cells of the tissue (brushings of the respiratory tract, sputum, skin biopsies, intestinal biopsies) are diverse (dendritic cells, innate lymphocytes, gamma delta-T cells). Basal stem cell analysis of airway and skin barrier place high demands on molecular differentiation with a small number of cells. In addition, there is strong autofluorescence in epithelial cells that can now be easily managed and even utilised for cell identification.The existing device was also acquired at the time through a DFG large-scale device application - the necessity of this equipment for the campus was beyond question. Unfortunately, the old device (after almost 10 years of use) no longer works reliably, causes immense maintenance costs and the lasers are also at the end of their life. However, the requested device is (in addition to the lower operating costs and replacement of the old one) also an important innovation element, as it removes technical limitations, opens up new research possibilities and will ultimately facilitate research with limited cell material and thus new impulses for clinical research at the Biederstein campus will put.

流式细胞仪技术已成为Klinikum rechts der Isar的Biederstein校园的“主要主力”。除了血液分析，以前被带入单细胞悬浮液的组织细胞也越来越多地被分析。免疫学研究的限制因素是并将继续限制人类细胞材料的获取，因此可用颜色的数量对Biederstein校园的所有研究人员来说都非常重要。因此，现在可能扩展到多达64种颜色是一个根本性的进步。即将到来的一个重要挑战是研究从组织中分离的细胞，将其置于单细胞悬浮液中，并使用表面标记物用流式细胞术进行表征。此外，基于分泌产物的功能参数允许功能性结论。组织的组织细胞和先天免疫细胞（呼吸道刷拭、痰、皮肤活检、肠活检）是多样的（树突状细胞、先天淋巴细胞、γ δ-T细胞）。气道和皮肤屏障的基础干细胞分析对少量细胞的分子分化提出了很高的要求。此外，上皮细胞中存在强烈的自发荧光，现在可以轻松管理，甚至用于细胞识别。现有的设备也是通过DFG大规模设备应用获得的-该设备对校园的必要性是毋庸置疑的。不幸的是，旧设备（经过近10年的使用）不再可靠地工作，导致巨大的维护成本，激光器也处于寿命的尽头。然而，所要求的设备（除了降低运营成本和更换旧设备之外）也是一个重要的创新元素，因为它消除了技术限制，开辟了新的研究可能性，并最终将促进有限细胞材料的研究，从而为Biederstein校园的临床研究带来新的动力。