Accelerating the genetic improvement of grain legumes for Australia by developing doubled haploid technology for field pea and chickpea.

通过开发豌豆和鹰嘴豆的双单倍体技术，加速澳大利亚谷物豆类的遗传改良。

• 批准号: LP0562111
• 负责人: Prof Kadambot Siddique
• 金额: $ 26.23万
• 依托单位: The University of Western Australia
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2005
• 资助国家: 澳大利亚
• 起止时间: 2005-12-31 至 2010-01-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0562111
• 关键词: Accelerating; genetic; improvement; grain; legumes

Doubled haploid technology is used in many broad acre crop species to accelerate cultivar development and create homozygous populations for genetic mapping. Field pea and chickpea have been unresponsive to this technique but a recent breakthrough by UWA researchers has resulted in haploid pro-embryos from in vitro cultured immature pollen. A barrier to further embryo maturation has been identified, which we propose to overcome using powerful microscopy tools to elucidate haploid embryology processes. This information will be applied to develop world-first in vitro doubled haploid protocols for these species, which will facilitate the development and accelerated delivery to industry of better adapted, high yielding cultivars.

双单倍体技术用于许多大面积作物品种，以加速栽培品种的开发和创建遗传作图的纯合群体。豌豆和鹰嘴豆对这种技术没有反应，但西澳大学的研究人员最近取得了突破，从体外培养的未成熟花粉中获得了单倍体原胚。进一步的胚胎成熟的障碍已被确定，我们建议克服使用强大的显微镜工具来阐明单倍体胚胎学过程。这些信息将用于开发世界上第一个针对这些物种的体外加倍单倍体方案，这将有助于开发和加速向工业提供更好的适应性，高产品种。