MALDI-TOF/TOF mass spectrometer

MALDI-TOF/TOF质谱仪

• 批准号: 491708830
• 金额: --
• 依托单位: Freie Universität Berlin
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/491708830?language=en
• 关键词: MALDI; TOF; mass; spectrometer

A high-resolution MALDI-TOF/TOF mass spectrometer is to be procured for the Bio-Mass Spectrometry (Bio-MS) unit in the Department of Biology, Chemistry, Pharmacy at Freie Universität Berlin. This will replace the Bruker Ultraflex II MALDI-TOF/TOF, which was applied for in 2006 and has been an indispensable part of the Bio-MS unit ever since. The MALDI-TOF/TOF technology complements the two LC-coupled ESI-Orbitrap-MS instruments, so that a wide range of analytical problems can be addressed. The MALDI-TOF technology is particularly suitable for rapid and robust identification and characterization of a broad spectrum of biomolecules as well as other substance classes (e.g. polymers) and often provides complementary results to the ESI-MS technology. For many applications, which are described in detail in the application, a combination of both technologies is therefore advantageous. For highly complex samples, which are typically present in classical proteomics experiments, chromatographic separation of the peptides is necessary, so LC-ESI-MS technology is used here. In contrast, for less complex samples, MALDI technology without chromatographic separation is usually preferred. This is mainly due to the direct fast analysis and the simplicity of the generated spectra. Also, the theoretically unlimited mass range of the TOF mass analyzer is particularly conducive to the analysis of large proteins or molecular assemblies. Especially for electrochemically unstable analytes, the use of MALDI is also more suitable compared to ESI, since oxidation and reduction processes can occur at the metal capillary during the ESI process. In order to be able to optimally deal with the numerous and diverse questions of the different working groups, equipping our facility with both technologies (MALDI-TOF as well as LC-ESI-Orbitrap) is therefore essential. After 15 years of operation, the currently used instrument is no longer state of the art in terms of speed, resolution and sensitivity, and support from Bruker is no longer guaranteed since 2017. In addition to the use of the instrument as part of the BioSupraMol instrument facility (Bio-MS area), some groups have a special need that is covered by a separate usage share. Detailed project descriptions can be found in section 3. The use as part of the BioSupraMol instrument center is outlined in the operation and use concept in section 2.2.

将为柏林自由大学生物、化学、药学系的生物质谱（Bio-MS）单位采购一台高分辨率MALDI-TOF/TOF质谱仪。这将取代布鲁克Ultraflex II MALDI-TOF/TOF，后者于2006年申请，此后一直是Bio-MS单元不可或缺的一部分。MALDI-TOF/TOF技术是对两种LC耦合ESI-Orbitrap-MS仪器的补充，因此可以解决广泛的分析问题。MALDI-TOF技术特别适用于快速和稳健地鉴定和表征广谱生物分子以及其他物质类别（例如聚合物），并且通常提供与ESI-MS技术互补的结果。因此，对于在本申请中详细描述的许多应用，两种技术的组合是有利的。对于典型存在于经典蛋白质组学实验中的高度复杂的样品，肽的色谱分离是必要的，因此这里使用LC-ESI-MS技术。相比之下，对于不太复杂的样品，通常首选不进行色谱分离的MALDI技术。这主要是由于直接快速分析和生成的光谱的简单性。此外，TOF质量分析仪的理论上无限的质量范围特别有利于分析大的蛋白质或分子组装体。特别是对于电化学不稳定的分析物，与ESI相比，MALDI的使用也更合适，因为在ESI过程中，氧化和还原过程可以在金属毛细管中发生。因此，为了能够以最佳方式处理不同工作组的众多不同问题，为我们的设施配备这两种技术（MALDI-TOF和LC-ESI-Orbitrap）至关重要。经过15年的运行，目前使用的仪器在速度、分辨率和灵敏度方面已不再是最先进的，自2017年以来，布鲁克不再保证提供支持。除了将该仪器作为BioSupraMol仪器设施（Bio-MS区域）的一部分使用外，一些小组还具有特殊需求，这些需求由单独的使用份额覆盖。详细的项目说明见第3节。第2.2节的操作和使用概念中概述了作为BioSupraMol仪器中心一部分的使用。