RapifleX MALDI-TOF/TOF Mass Spectrometer

项目总结 此提案是重新提交的，请求为Bruker RapifleX基质辅助激光解吸提供资金 可进行MS成像的电离(MALDI)飞行时间/飞行时间(TOF/TOF)质谱仪 (MSI)，用于新墨西哥大学(UNM)综合分子分析核心设施。MSI是一款功能强大的 一种同时探测和绘制数百个已知和未知分子位置的技术 他们所在的州没有标签。RapifleX是市场上最先进的MALDI-TOF/TOF系统。 与其他商业化的分子MSI仪器相比，RapifleX使用 最高的重复激光频率和空间分辨率。最新一代的RapifleX系统包括新的 坚固耐用的设计，确保高数据完整性和长激光寿命。此外，请求的RapifleX TOF/TOF质量分析仪具有分析复杂生物分子所需的能力，这些生物分子对 使用我们目前的MALDI和电喷雾电离系统进行分析。这是一项基本的MS/MS功能 我们的化学生物学家的用户社区需要产生修饰的蛋白质/肽并开发 生物正交反应。目前，没有可用于执行这种类型的MSI和MS/MS的仪器 在新墨西哥州或新墨西哥州进行的分析。重要的是，RapifleX将补充现有的MS机队 UNM的仪器。该平台无缝集成了MALDI-TOF/TOF、LC耦合高分辨率MS、 激光消融电感耦合等离子体质谱用于空间引导组学以及分子和元素成像的组合。 因此，这个平台在我们的地理区域将是独一无二的，我们希望为调查人员提供服务，不仅来自 UNM还有来自周边机构的资金。这些创新能力将为 共享核心地区的资源，使其成为该地区的卓越中心。 我们来自几个用户的令人兴奋的新的初步结果突出了针对我们的 社区的社会健康问题，包括肝病、脑损伤和卵巢癌。我们的发现 不仅证明了选择RapifleX的合理性，还说明了MSI对我们的研究具有变革性的好处 项目。主要用户群覆盖4个学术部门和3个学院(药学、医学、艺术 和科学)，并代表基础和翻译研究兴趣。因此，添加 RapifleX将对许多由NIH资助的研究人员产生直接和持久的影响，这些研究 涵盖癌症、肥胖症、神经科学、心血管疾病和化学生物学。此外， 这款高性能MS仪器的推出将有助于开发新的教育平台，并促进 来自代表人数不足的群体的受训人员在为拉美裔服务的机构--UNM的研究经验。 重要的是，S10共享仪器将支持和协同三个方面的质谱学工作 美国国立卫生研究院机构发展奖(IDEA)生物医学研究卓越项目中心。

PROJECT SUMMARY This proposal is a resubmission requesting funding for a Bruker rapifleX matrix-assisted laser desorption ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometer (MS) capable of MS imaging (MSI) for the University of New Mexico (UNM) Integrative Molecular Analysis Core facility. MSI is a powerful technique that simultaneously detects and maps the location of hundreds of known and unknown molecules in their native state label-free. The rapifleX is the most advanced MALDI-TOF/TOF system on the market. Compared to other commercially available molecular MSI instruments, the rapifleX acquires images with the highest repetition lasing rate and spatial resolution. The latest generation of the rapifleX system includes new robust designs to ensure high data integrity and long laser lifetime. Additionally, the requested rapifleX TOF/TOF mass analyzer has the capabilities needed to analyze complex biomolecules that are challenging to analyze using our current MALDI and electrospray ionization systems. This is an essential MS/MS capability needed by our user community of chemical biologists generating modified proteins/peptides and developing bioorthogonal reactions. Currently, there is no instrument available to perform this type of MSI and MS/MS analysis at UNM or in the State of New Mexico. Importantly, the rapifleX will complement a fleet of existing MS instruments at UNM. The platform seamlessly integrates MALDI-TOF/TOF, LC coupled high-resolution MS, and laser ablation ICP-MS for spatially guided omics and a combination of molecular and elemental imaging. Thus, this platform will be unique in our geographic area, and we expect to serve investigators not only from UNM but also from surrounding institutions. These innovative capabilities will bring a new dimension to the shared resources at the Core, making it a center of excellence for the region. Our exciting new preliminary results from several users highlight a broad spectrum of research addressing our communities' societal health concerns, including liver disease, brain injury, and ovarian cancer. Our findings not only justify the choice of the rapifleX but also illustrate the transformative benefits of MSI on our research projects. The major user group extends over 4 academic departments & 3 Colleges (Pharmacy, Medicine, Arts and Sciences) and represents both basic and translational research interests. As such, the addition of the rapifleX will have an immediate and lasting impact on many NIH-funded investigators conducting research that spans cancer, obesity, neurosciences, cardiovascular diseases, and chemical biology. Moreover, the availability of this high performance MS instrument will help develop new educational platforms and boost the research experience of trainees from underrepresented groups at UNM, a Hispanic-serving institution. Importantly, this S10 Shared Instrumentation will support and synergize mass spectrometric efforts for three NIH Institutional Development Award (IDeA) Centers of Biomedical Research Excellence programs.