RapifleX MALDI-TOF/TOF Mass Spectrometer

RapifleX MALDI-TOF/TOF 质谱仪

• 批准号: 10630621
• 负责人: Changjian Feng
• 金额: $ 60万
• 依托单位: UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630621
• 关键词: Ablation; Address; Arts; Award; Basic Science; Biology; Brain Injuries; Cardiovascular Diseases; Centers of Research Excellence; Chemicals; Communities; Complement; Complex; Core Facility; Coupled; Development; Dimensions; Education; Electrospray Ionization; Elements; Ensure; Funding; Generations; Geographic Locations; Health; Hispanic-serving Institution; Image; Imaging Device; Inductively Coupled Plasma Mass Spectrometry; Institution; Label; Lasers; Liver diseases; Location; Malignant Neoplasms; Malignant neoplasm of ovary; Maps; Marketing; Medicine; Molecular; Molecular Analysis; Neurosciences; New Mexico; Obesity; Peptides; Performance; Pharmacy facility; Proteins; Reaction; Research; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Science; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; System; Techniques; Time; Translational Research; Underrepresented Populations; United States National Institutes of Health; Universities; college; data integrity; design; experience; innovation; instrument; instrumentation; interest; mass analyzer; mass spectrometer; molecular mass; programs; synergism

PROJECT SUMMARY This proposal is a resubmission requesting funding for a Bruker rapifleX matrix-assisted laser desorption ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometer (MS) capable of MS imaging (MSI) for the University of New Mexico (UNM) Integrative Molecular Analysis Core facility. MSI is a powerful technique that simultaneously detects and maps the location of hundreds of known and unknown molecules in their native state label-free. The rapifleX is the most advanced MALDI-TOF/TOF system on the market. Compared to other commercially available molecular MSI instruments, the rapifleX acquires images with the highest repetition lasing rate and spatial resolution. The latest generation of the rapifleX system includes new robust designs to ensure high data integrity and long laser lifetime. Additionally, the requested rapifleX TOF/TOF mass analyzer has the capabilities needed to analyze complex biomolecules that are challenging to analyze using our current MALDI and electrospray ionization systems. This is an essential MS/MS capability needed by our user community of chemical biologists generating modified proteins/peptides and developing bioorthogonal reactions. Currently, there is no instrument available to perform this type of MSI and MS/MS analysis at UNM or in the State of New Mexico. Importantly, the rapifleX will complement a fleet of existing MS instruments at UNM. The platform seamlessly integrates MALDI-TOF/TOF, LC coupled high-resolution MS, and laser ablation ICP-MS for spatially guided omics and a combination of molecular and elemental imaging. Thus, this platform will be unique in our geographic area, and we expect to serve investigators not only from UNM but also from surrounding institutions. These innovative capabilities will bring a new dimension to the shared resources at the Core, making it a center of excellence for the region. Our exciting new preliminary results from several users highlight a broad spectrum of research addressing our communities' societal health concerns, including liver disease, brain injury, and ovarian cancer. Our findings not only justify the choice of the rapifleX but also illustrate the transformative benefits of MSI on our research projects. The major user group extends over 4 academic departments & 3 Colleges (Pharmacy, Medicine, Arts and Sciences) and represents both basic and translational research interests. As such, the addition of the rapifleX will have an immediate and lasting impact on many NIH-funded investigators conducting research that spans cancer, obesity, neurosciences, cardiovascular diseases, and chemical biology. Moreover, the availability of this high performance MS instrument will help develop new educational platforms and boost the research experience of trainees from underrepresented groups at UNM, a Hispanic-serving institution. Importantly, this S10 Shared Instrumentation will support and synergize mass spectrometric efforts for three NIH Institutional Development Award (IDeA) Centers of Biomedical Research Excellence programs.

项目摘要 该提案是一份重新提交的申请，要求为布鲁克rapifleX基质辅助激光解吸提供资金。 能够进行MS成像的电离（MALDI）飞行时间/飞行时间（TOF/TOF）质谱仪（MS） (MSI)新墨西哥州大学（UNM）整合分子分析核心设施。MSI是一个强大的 一种同时检测和绘制数百种已知和未知分子位置的技术， 他们的家乡没有标签。rapifleX是市场上最先进的MALDI-TOF/TOF系统。 与其他市售分子MSI仪器相比，rapifleX使用 最高的重复频率和空间分辨率。最新一代rapifleX系统包括新的 坚固的设计，确保高数据完整性和长激光寿命。此外，请求的rapifleX TOF/TOF质量分析仪具有分析复杂生物分子所需的能力，这些生物分子具有挑战性， 使用我们目前的MALDI和电喷雾电离系统进行分析。这是MS/MS的基本功能 我们的化学生物学家用户社区需要生成修饰的蛋白质/肽， 生物正交反应目前，没有仪器可用于执行这种类型的MSI和MS/MS 在UNM或新墨西哥州进行分析。重要的是，rapifleX将补充现有的MS车队 仪器在UNM该平台无缝集成了MALDI-TOF/TOF，LC耦合高分辨率MS， 和激光烧蚀ICP-MS用于空间引导组学以及分子和元素成像的组合。 因此，这个平台在我们的地理区域将是独一无二的，我们希望不仅为来自世界各地的研究人员提供服务， 也包括周边的机构。这些创新能力将为 在核心共享资源，使其成为该地区的卓越中心。 我们来自几个用户的令人兴奋的新的初步结果突出了广泛的研究， 社区的社会健康问题，包括肝病，脑损伤和卵巢癌。我们的研究结果 不仅证明了rapifleX的选择，而且还说明了MSI对我们研究的变革性好处 项目主要用户群扩展到4个学术部门和3个学院（药学，医学，艺术 和科学），并代表了基础和转化的研究兴趣。因此，增加 rapifleX将对许多NIH资助的研究人员产生直接和持久的影响， 涵盖癌症、肥胖症、神经科学、心血管疾病和化学生物学。而且 这种高性能MS仪器的可用性将有助于开发新的教育平台， 研究经验的学员从代表性不足的群体在墨西哥国立大学，一个西班牙裔服务机构。 重要的是，S10共享仪器将支持和协同质谱工作， NIH机构发展奖（IDEA）生物医学研究卓越计划中心。