Molecular functions of class II Glycine-rich proteins (GRPs) in plant parasitism and plant immunity
II类富含甘氨酸的蛋白(GRP)在植物寄生和植物免疫中的分子功能
基本信息
- 批准号:492015347
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:
- 资助国家:德国
- 起止时间:
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Cuscuta spp. (dodder) are root- and leaf-less holoparasitic plants which infect dicotyledonous plants by winding around the shoots and penetrating host tissues. With multicellular infection organs, called haustoria, Cuscuta spp. connect to the host’s vasculature and withdraw water, solutes, nutrients and carbohydrates. In this manner, dodders harm host plants and pose an extra threat to many crops in addition to other phytopathogens and herbivores. Cultivated tomato as one notable exception displays an active defense against Cuscuta reflexa including immune responses like the induction of ethylene, reactive-oxygen species as well as a type of hypersensitive response (HR), in common finally leading to a full resistance of tomato against C. reflexa. In previous works we identified the leucine-rich repeat receptor protein Cuscuta Receptor 1 (CuRe1) as a critical component of tomato’s immune system to recognize C. reflexa and to induce the defense responses described. As a pathogen-associated molecular pattern (PAMP) of the parasitic plant C. reflexa, we recently identified a C. reflexa glycine-rich protein (CrGRP) that localizes to the parasite’s cell walls and that seems present in all parts and tissues of Cuscuta plants. CrGRP physically interacts with CuRe1 and we identified a 21 amino acid long peptide epitope, crip21 (cysteine-rich peptide 21), that is sufficient to bind to CuRe1 and to induce tomato immune responses in nanomolar ranges. After BLAST-analyses, homologs of CrGRPs can be found in all other organisms (microbes, fungi, arthropods and vertebrates) and other plants, even in tomato. The Solanum lycopersicum GRP (SlGRP) or Slcrip21 peptide, however, was not able to be recognized by tomato CuRe1 and we identified a single aa residue (Tyr11) that abolishes its function as defense-trigger and also demonstrates that CrGRP is not acting as potential damage-associated molecular pattern (DAMP). The original function of CrGRP and SlGRP are unknown and after literature research, it turned out that in general not much is known about the molecular function or physiological role of such GRPs and derived peptides in plants. In the proposed work, we therefore would like to address the following questions; i) What functions has CrGRP for Cuscuta spp. and how are those related to susceptibility or resistance of hosts? ii) what physiological roles and molecular functions do have GRPs for host plants in general and during an ongoing infection process by dodder? iii) How do GRPs associate with other proteins or (cell wall) components and does it matter for Cuscuta – host interaction? iv) Peptide motifs with high similarity to crip21 can be found in other parasitic plants, in insect or in microbial phytopathogens – do they have any function as trigger or suppressor of plant defense? and v) How are potential receptors for crip21 are distributed among the Solanaceae and how efficient do they recognize crip21-like peptides from other phytopathogens?
菟丝子属(菟丝子)是无根无叶的全寄生植物,其通过缠绕在芽周围并穿透宿主组织来感染双子叶植物。菟丝子属(Cuscuta spp.)连接到宿主的脉管系统并提取水、溶质、营养物和碳水化合物。以这种方式,除了其他植物病原体和草食动物外,菟丝子还会危害宿主植物并对许多作物构成额外的威胁。栽培番茄是一个显著的例外,表现出对Cuscuta reflexa的积极防御,包括乙烯诱导的免疫反应、活性氧以及一种过敏反应(HR),共同最终导致番茄对Cuscuta reflexa的完全抗性。反射在以前的工作中,我们确定了富含亮氨酸的重复受体蛋白菟丝子受体1(CuRe 1)作为番茄免疫系统识别C。反射并诱导所述的防御反应。病原相关分子模式(PAMP)是寄生植物C. reflexa,我们最近发现了一个C。reflexa富含甘氨酸的蛋白质(CrGRP),定位于寄生虫的细胞壁,似乎存在于菟丝子植物的所有部位和组织中。CrGRP与CuRe 1物理相互作用,我们确定了一个21个氨基酸长的肽表位,crip 21(富含半胱氨酸的肽21),这是足以结合到CuRe 1,并诱导番茄免疫反应纳摩尔范围。BLAST分析后,CrGRP的同源物可以在所有其他生物(微生物,真菌,节肢动物和脊椎动物)和其他植物中找到,甚至在番茄中。然而,番茄GRP(SlGRP)或Slcrip 21肽不能被番茄CuRe 1识别,我们确定了一个单一的氨基酸残基(Tyr 11),废除其作为防御触发器的功能,也表明CrGRP不是作为潜在的损伤相关分子模式(DAMP)。CrGRP和SlGRP的原始功能是未知的,并且在文献研究之后,事实证明,通常对植物中的此类GRP和衍生肽的分子功能或生理作用知之甚少。因此,在拟议的工作中,我们希望解决以下问题:i)CrGRP对菟丝子属物种有什么功能?它们与宿主的易感性或抗性有何关系?ii)GRP在一般情况下和在菟丝子持续感染过程中对宿主植物有哪些生理作用和分子功能?iii)GRP如何与其他蛋白质或(细胞壁)组分结合,以及它对菟丝子-宿主相互作用是否重要?iv)与crip 21高度相似的肽基序可以在其他寄生植物、昆虫或微生物植物病原体中发现-它们是否具有作为植物防御的触发剂或抑制剂的任何功能?和v)crip 21的潜在受体在茄科中是如何分布的,它们识别来自其他植物病原体的crip 21样肽的效率如何?
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Professor Dr. Markus Albert其他文献
Professor Dr. Markus Albert的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Professor Dr. Markus Albert', 18)}}的其他基金
Parasitic molecular cues during plant-plant interaction - recognition and influence on host plants
植物与植物相互作用过程中的寄生分子线索——对寄主植物的识别和影响
- 批准号:
350258880 - 财政年份:2017
- 资助金额:
-- - 项目类别:
Research Grants
Defence-triggering molecules of the parasitic plant Cuscuta and the recognition by cell surface receptors
寄生植物菟丝子的防御触发分子及其细胞表面受体的识别
- 批准号:
194018311 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Research Grants
Heterotrophic Nutrition Strategies of the Parasitic Plant Cuscuta
寄生植物菟丝子的异养营养策略
- 批准号:
523910401 - 财政年份:
- 资助金额:
-- - 项目类别:
Research Grants
相似国自然基金
数学物理中精确可解模型的代数方法
- 批准号:11771015
- 批准年份:2017
- 资助金额:48.0 万元
- 项目类别:面上项目
相似海外基金
Theory and algorithms for a new class of computationally amenable nonconvex functions
一类新的可计算非凸函数的理论和算法
- 批准号:
2416250 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant
Theory and algorithms for a new class of computationally amenable nonconvex functions
一类新的可计算非凸函数的理论和算法
- 批准号:
2309729 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Standard Grant
Role of RIPK2 in the neuroinflammatory response to ischemic stroke
RIPK2 在缺血性卒中神经炎症反应中的作用
- 批准号:
10680081 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Novel functions for Sm-class RNAs in the regulation of gene expression
Sm 类 RNA 在基因表达调控中的新功能
- 批准号:
10330827 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Novel functions for Sm-class RNAs in the regulation of gene expression
Sm 类 RNA 在基因表达调控中的新功能
- 批准号:
10578942 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Novel functions for Sm-class RNAs in the regulation of gene expression
Sm 类 RNA 在基因表达调控中的新功能
- 批准号:
10574621 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Treatment of Primary Amoebic Meningoencephalitis via Modulation of Antibody Effector Functions
通过调节抗体效应器功能治疗原发性阿米巴脑膜脑炎
- 批准号:
10550175 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Treatment of Primary Amoebic Meningoencephalitis via Modulation of Antibody Effector Functions
通过调节抗体效应器功能治疗原发性阿米巴脑膜脑炎
- 批准号:
10179955 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Treatment of Primary Amoebic Meningoencephalitis via Modulation of Antibody Effector Functions
通过调节抗体效应器功能治疗原发性阿米巴脑膜脑炎
- 批准号:
10374907 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Protective lung memory B cell functions and dynamics during respiratory infection
呼吸道感染期间保护性肺记忆 B 细胞的功能和动态
- 批准号:
10621961 - 财政年份:2021
- 资助金额:
-- - 项目类别: