Purification and ampliefication of hematopoietic stem cells

造血干细胞的纯化和扩增

基本信息

  • 批准号:
    06277104
  • 负责人:
  • 金额:
    $ 128.83万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 1998
  • 项目状态:
    已结题

项目摘要

Soda, Miura and Kanakura have shown that some signal transduction pathways in hemopoictic stem cells (HSC). Novel receptor tyrosine kinases such as TIEs, HTK and STK/RON were cloned from purified HSC by RT-PCR and especially TIE-1 and 2 are expressed in hematopotetic stem cells as well as in vascular endothelial cells From these findings, it becomes possible to analyze the development of hematopoietic stem cells from angioblasts, which are endothelial precursor cells. It was clarified that gain of function of c Kit caused the autonomous proliferation of hematopoietic cells (mast cells) and specified cells in the gut (Kajal cells).Nakauchi has demonstrated that CD34tィイD1+ィエD1KitィイD1+ィエD1ScaィイD1+ィエD1LinィイD1-ィエD1 and CD34ィイD1+ィエD1KitィイD1+ィエD1ScaィイD1+ィエD1LinィイD1-ィエD1 cells of adult murine bone marrow have been shown to contain long-and short-term repopulating hematopoictic stem cells by a transplantation assay. CD34 negative stein cells are also confirmed in human hematopoletic system. There has been great interest in the ex vivo expansion of human hemopoictic stem/progenitor cells for clinical applications. Nakahata has shown that heanatopoietic progenitor cellscan be expanded in the presence of soluble IL-6 receptor+IL-6+SCF. However. stein cell expansion is still insatisfactoiy. Asano has tried to establish monkey transplantation system to analyze the hurtan HSC.Yamamoto has clearly shown that GATA and Maf families am involved in erythropoiesis by the GATA-1 KO mice and GATA promoter-LacZ transgenic mice.
Soda, Miura和Kanakura已经证明了造血干细胞(HSC)中的一些信号转导途径。通过RT-PCR从纯化的HSC中克隆出新的酪氨酸受体激酶,如TIEs、HTK和STK/RON,特别是TIE-1和2在造血干细胞和血管内皮细胞中表达。这些发现使得分析造血干细胞从内皮前体细胞成血管细胞发育成为可能。阐明了c Kit功能的获得可引起造血细胞(肥大细胞)和肠道特定细胞(Kajal细胞)的自主增殖。Nakauchi通过移植实验证明,成年小鼠骨髓中的CD34t γ γ γ γ γ + γ γ γ γ + γ γ γ + γ γ γ + γ γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ + γ γ γ - γ γ - γ γ - γ γ细胞含有长期和短期再生造血干细胞。CD34阴性的stein细胞也在人造血系统中得到证实。人类造血干细胞/祖细胞的体外扩增用于临床应用已经引起了人们极大的兴趣。Nakahata已经证明造血祖细胞在可溶性IL-6受体+IL-6+SCF存在下可以扩增。然而。斯坦因细胞扩增仍不令人满意。浅野试图建立猴子移植系统来分析人体造血干细胞。Yamamoto清楚地表明GATA和Maf家族参与了GATA-1 KO小鼠和GATA启动子- lacz转基因小鼠的红细胞生成。

项目成果

期刊论文数量(69)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
HiraO A: "Translocation of the Csk homologous kinase(Chk/Hyl)controls activity of CD36-anchored Lyn tyrosine in thrombin-stimulated platelets." EMBO J. 16. 2342-2351 (1997)
HiraO A:“Csk 同源激酶 (Chk/Hyl) 的易位控制了凝血酶刺激的血小板中 CD36 锚定的 Lyn 酪氨酸的活性。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Iwama A et al.: "Molecular cloning of a novel receptor tyrosine kinase gene,STK,derived from enriched hematopoietic stem cells." Blood. 83. 3160-3169 (1994)
Iwama A 等人:“源自富集造血干细胞的新型受体酪氨酸激酶基因 STK 的分子克隆。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Itoh K et al: "Cloning and characterization of a novel erythroid-derived CNC family transcription factor heterodimerizing with the small Maf." Mol.Cell.Biol. 15. 4184-4193 (1995)
Itoh K 等人:“与小 Maf 异二聚化的新型红系衍生 CNC 家族转录因子的克隆和表征。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Komatsu N et al: "In vitro development of erythrold and megakaryocytic cells from a UT-7 subline, UT-7/GM"Blood. 89. 4021-4033 (1997)
Komatsu N 等人:“来自 UT-7 亚系 UT-7/GM 的红细胞和巨核细胞的体外发育”血液。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Matsuzaki Y.: "Role of bcl-2 in the Development of Lymphoid Cells From the Hematopoietic Stem Cell." Blood. 89. 853-862 (1997)
Matsuzaki Y.:“bcl-2 在造血干细胞发育成淋巴细胞中的作用。”
  • DOI:
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  • 期刊:
  • 影响因子:
    0
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SUDA Toshio其他文献

SUDA Toshio的其他文献

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{{ truncateString('SUDA Toshio', 18)}}的其他基金

Self-renewal capacity of hematopoietic stem cells and mitochondrial metabolism
造血干细胞的自我更新能力与线粒体代谢
  • 批准号:
    18H05284
  • 财政年份:
    2018
  • 资助金额:
    $ 128.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Homeostasis of hematopoietic stem cells and its breakdown
造血干细胞的稳态及其分解
  • 批准号:
    26221309
  • 财政年份:
    2014
  • 资助金额:
    $ 128.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Nicher Regulation for Normal Hemopoietic Stem Cells and Leukemic Stem Cells
正常造血干细胞和白血病干细胞的利基调节
  • 批准号:
    23249053
  • 财政年份:
    2011
  • 资助金额:
    $ 128.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Regulation of Hematopoietic Stem Cell Division in a Niche
微环境中造血干细胞分裂的调节
  • 批准号:
    16002011
  • 财政年份:
    2004
  • 资助金额:
    $ 128.83万
  • 项目类别:
    Grant-in-Aid for Specially Promoted Research
Differentiation of Mesenchymal Stem Cells in Bone Marrow
骨髓间充质干细胞的分化
  • 批准号:
    12557082
  • 财政年份:
    2000
  • 资助金额:
    $ 128.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular analysis for microenvironment of hematopoietic cells.
造血细胞微环境的分子分析。
  • 批准号:
    10307024
  • 财政年份:
    1998
  • 资助金额:
    $ 128.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A).
Self Renew and Cell Differentiation in Hemopoietic System
造血系统的自我更新和细胞分化
  • 批准号:
    10181103
  • 财政年份:
    1998
  • 资助金额:
    $ 128.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Cloning and functional analysis of specific molecules of osteoclast
破骨细胞特定分子的克隆及功能分析
  • 批准号:
    09557086
  • 财政年份:
    1997
  • 资助金额:
    $ 128.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Functional analyzes for common receptor tyrosine kinases of hematopoietic stem cells and vascular endothelial cells
造血干细胞和血管内皮细胞共同受体酪氨酸激酶的功能分析
  • 批准号:
    08457279
  • 财政年份:
    1996
  • 资助金额:
    $ 128.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Cloning of Seel-renewal factor receptors from hemopoietic stem cells
造血干细胞 Seel 更新因子受体的克隆
  • 批准号:
    05454333
  • 财政年份:
    1993
  • 资助金额:
    $ 128.83万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
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