Molecular analysis for microenvironment of hematopoietic cells.
造血细胞微环境的分子分析。
基本信息
- 批准号:10307024
- 负责人:
- 金额:$ 25.15万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (A).
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The association between hemopoietic stem cells (HSCs) and their microenvironment is central to the development and maintenance of a functional hematopoietic system.We clarified following 4 points.(1) We have investigated the function of TIE2 in the development of definitive hematopoiesis during embryogenesis. In vitellin artery, TIE2^+ hemopoietic cells aggregated and adhered to TIE2^+ endothelial cells (Immunity, 1998). Single TIE2^+ cells generated hematopoietic cells and endothelial cells(ECs) simultaneously. This TIE2^+ cells is identified as hemangioblasts(Blood, 1999).(2) A TIE2-ligand, angiopoietin-1 (Ang-1) promoted the adhesion of TIE2^+ HSCs to fibronectin through integrins. This adhesion enhances not only the proliferation of hematopoietic progenitor cells but also the maintenance of stemness (Immunity, 1998).(3) We found that Ang-1 derived from TIE2^+ HSCs promoted vessel sprouting into avascular areas (Cell, 2000). This suggests a close interaction between HSCs and ECs.(4) IL-3-dependent BaF cells transfected constitutive active form of TIE2 receptor kinase represented long survival without IL-3. This indicates two possibilities ; one is the changes of cell cycle by adhesion and the other is the avoidance of apoptosis through TIE2 signaling. It is likely that hemopoietic stem cells forms their own microenvironment (Niche) by the adhesion to stromal cells or cell matrix.
造血干细胞(HSCs)与其微环境之间的联系对于造血系统的发育和维持至关重要。(1)我们已经研究了TIE 2在胚胎发生过程中确定性造血发育中的功能。在卵黄蛋白动脉中,TIE 2 ^+造血细胞聚集并粘附于TIE 2 ^+内皮细胞(Immunity,1998)。单个TIE 2 ^+细胞可同时生成造血细胞和内皮细胞。这种TIE 2 ^+细胞被鉴定为成血管细胞(Blood,1999)。(2)TIE 2配体血管生成素-1(Ang-1)通过整合素促进TIE 2 ^+ HSC与纤维连接蛋白的粘附。这种粘附不仅增强造血祖细胞的增殖,而且还增强干细胞的维持(Immunity,1998)。(3)我们发现来源于TIE 2 ^+ HSC的Ang-1促进血管出芽进入无血管区(Cell,2000)。这表明HSC和EC之间存在密切的相互作用。(4)IL-3依赖性BaF细胞转染组成型活性形式的TIE 2受体激酶表示长期生存没有IL-3。这表明两种可能性;一种是通过粘附改变细胞周期,另一种是通过TIE 2信号传导避免凋亡。造血干细胞可能通过粘附于基质细胞或细胞基质而形成自身的微环境(Niche)。
项目成果
期刊论文数量(53)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nakamura YS: "Tyro 3 receptor tyrosine kinase and its ligand, Gas6, stimulate the function of osteoclasts."Stem Cells. 16. 229-238 (1998)
Nakamura YS:“Tyro 3 受体酪氨酸激酶及其配体 Gas6 刺激破骨细胞的功能。”干细胞。
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- 影响因子:0
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- 通讯作者:
Takakura N: "Critical role of the TIE2 endothelial cell receptor in the development of definitive hematopoiesis."Immunity. 9. 677-686 (1998)
Takakura N:“TIE2 内皮细胞受体在最终造血发育中的关键作用。”免疫。
- DOI:
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- 影响因子:0
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Duan Y: "Expression and functional analysis of hemopoietic progenitor antigen. NJ-1(114/A10), in the megakaryocytic lineage."Biochem Biophys Res Comm. 253. 401-406 (1998)
段Y:“造血祖抗原的表达和功能分析。NJ-1(114/A10),在巨核细胞谱系中。”Biochem Biophys Res Comm。
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- 影响因子:0
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Takakura N, Watanabe T, Suenobu S, Yamada Y, Noda T, Ito Y, Satake M and Suda T: "A role for hematopoietic cells in promoting angiogenesis."Cell. 102. 199-209 (2000)
Takakura N、Watanabe T、Suenobu S、Yamada Y、Noda T、Ito Y、Satake M 和 Suda T:“造血细胞在促进血管生成中的作用。”细胞。
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- 影响因子:0
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Hirao A: "Overexpression of c-terminal src kinase homologous kinase supresses activation of lyntyrosine kinase required for VLA5-mediated Dami cell spreading." J Biol Chem. 273. 10004-10010 (1998)
Hirao A:“C 端 src 激酶同源激酶的过度表达会抑制 VLA5 介导的 Dami 细胞扩散所需的林酪氨酸激酶的激活。”
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{{ truncateString('SUDA Toshio', 18)}}的其他基金
Self-renewal capacity of hematopoietic stem cells and mitochondrial metabolism
造血干细胞的自我更新能力与线粒体代谢
- 批准号:
18H05284 - 财政年份:2018
- 资助金额:
$ 25.15万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Homeostasis of hematopoietic stem cells and its breakdown
造血干细胞的稳态及其分解
- 批准号:
26221309 - 财政年份:2014
- 资助金额:
$ 25.15万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Nicher Regulation for Normal Hemopoietic Stem Cells and Leukemic Stem Cells
正常造血干细胞和白血病干细胞的利基调节
- 批准号:
23249053 - 财政年份:2011
- 资助金额:
$ 25.15万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Regulation of Hematopoietic Stem Cell Division in a Niche
微环境中造血干细胞分裂的调节
- 批准号:
16002011 - 财政年份:2004
- 资助金额:
$ 25.15万 - 项目类别:
Grant-in-Aid for Specially Promoted Research
Differentiation of Mesenchymal Stem Cells in Bone Marrow
骨髓间充质干细胞的分化
- 批准号:
12557082 - 财政年份:2000
- 资助金额:
$ 25.15万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Self Renew and Cell Differentiation in Hemopoietic System
造血系统的自我更新和细胞分化
- 批准号:
10181103 - 财政年份:1998
- 资助金额:
$ 25.15万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Cloning and functional analysis of specific molecules of osteoclast
破骨细胞特定分子的克隆及功能分析
- 批准号:
09557086 - 财政年份:1997
- 资助金额:
$ 25.15万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional analyzes for common receptor tyrosine kinases of hematopoietic stem cells and vascular endothelial cells
造血干细胞和血管内皮细胞共同受体酪氨酸激酶的功能分析
- 批准号:
08457279 - 财政年份:1996
- 资助金额:
$ 25.15万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Purification and ampliefication of hematopoietic stem cells
造血干细胞的纯化和扩增
- 批准号:
06277104 - 财政年份:1994
- 资助金额:
$ 25.15万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Cloning of Seel-renewal factor receptors from hemopoietic stem cells
造血干细胞 Seel 更新因子受体的克隆
- 批准号:
05454333 - 财政年份:1993
- 资助金额:
$ 25.15万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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