Establishment of a nationwide NTF-EV-Biobank from polytraumatized patients: dependency of EV-characteristics in response to the injury pattern

2013年，由创伤研究网络工作队(Netzwerk Traumaforschung，NTF)(Relja等人)发起了全国多发性创伤患者血清和血浆样本生物库。2020年；Huber-Lang等人。2011年)。这一质量控制项目是为了系统地评估和监测多发伤患者的(病理)生理构成和临床治疗策略，以提高他们的水平。在2018年结束的五年试点阶段，多中心NTF生物库以及在线工具的可行性已得到成功证明，并在七个创伤中心建立。在下一个资助期，将建立一个非关税壁垒-EV-生物库。本项目的目标是用NTF-EV-生物库扩展NTF生物库，并提供创伤患者血清/血浆EV的分离、存储和管理方案。这包括有效隔离电动汽车的方法的比较，将伦理和软件基础扩展到额外的电动汽车隔离和储存。因此，第一个NTF-EV-Biobank先导研究的目的是验证所开发的EV生物库的可行性，并将重点放在比较不同损伤类型的患者在创伤后不同时间点收集的血清/血浆中的EV种群。因此，入院的EVS样本将根据新的NTF-EV-生物库分离和鉴定方案进行采集。NTF-EV-Biobank材料将用于通过给定的胞外细胞表面受体追踪组织细胞起源的EV。表面受体EV条形码可以反映损伤模式，因此可能成为未来的诊断工具。此外，来自新成立的NTF-EV-Biobank的电动汽车将用于分析电动汽车货物。特别是，假设短半衰期的炎症标志物不会在EVS中降解，因此可以作为多发伤患者的诊断和预后标志物。

The nationwide biobank for sera and plasma samples from polytrauma patients was initiated in 2013 by the task force Network Trauma Research (Netzwerk Traumaforschung, NTF) (Relja et al. 2020; Huber-Lang et al. 2011). This quality control project was introduced to systemically evaluate and monitor polytraumatized patients in terms of their (patho-) physiological make-up and clinical treatment strategies in order to improve the same. The feasibility of the multi-center NTF Biobank as well as the online tool have been successfully proven and established in seven trauma centers during the pilot phase of five years, which ended in 2018. In the next funding period, the establishment of an NTF-EV-Biobank will be conducted. The objective of this project is to expand the NTF Biobank with NTF-EV-Biobank and provide isolation, storage and management protocols of serum-/plasma- EVs from trauma patients. This includes comparisons of methods for efficient EV isolation, extension of the ethics and software basis to the additional isolation and storage of EVs. Consequently, the first NTF-EV-Biobank pilot study will be aimed to validate the feasibility of the developed EV Biobank and will be focused on comparison of EVs populations in serum/plasma of patients with different injury patterns, collected at different time points after trauma. Therefore, EVs samples from admission to the hospital will be collected according to the new NTF-EV-Biobank isolation and characterization protocol. The NTF-EV-Biobank material will be used to trace tissue-cell-originated EVs by given exosomal cell surface receptors. The surface receptor EV barcode could mirror the injury pattern and therefore could be a diagnostic tool in future. In addition, the EVs from the newly established NTF-EV-Biobank will be used to analyse the EVs cargo. Especially, the short half-life inflammatory markers are hypothesized to be preserved from degradation in EVs and therefore could be used as a diagnostic and prognostic markers in polytrauma patients