Large Scale Sequencing to Unravel Carbon Cycling in the Elbe estuary (Micro)biota

大规模测序揭示易北河河口（微）生物群的碳循环

• 批准号: 496691966
• 负责人: Professorin Dr. Kathrin Dausmann
• 金额: --
• 依托单位: Institut für marine Ökosystem- und Fischereiwissenschaften
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/496691966?language=en
• 关键词: Large; Scale; Sequencing; Unravel; Carbon

Estuaries cover more than one fifth of the global terrestrial surfaces and are key drives in global carbon fixation. They are characterized by high temporal dynamics due to variable external forcing (e.g. freshwater discharge, ocean currents, tidal energy) and by pronounced spatial gradients in, e.g., salinity, hydrodynamics, and oxygen availability (estuarine gradients). Along estuarine gradients, ecosystems of intertidal areas (vegetated tidal marshes, intertidal flats with microphytobenthos communities) and subtidal channels (benthic and pelagic ecosystems) are tightly linked and produce, transport and process large amounts of terrestrial and aquatic organic matter. Key processes governing estuarine C cycling are still poorly understood, although recent research has exemplified the potential high relevance of plant- and phytoplankton-microbe interactions and herbivory as controls of C fluxes in tidal marshes and estuarine channels. The proposal together with the RTG 2530 aims to systematically analyze biota-mediated effects on estuarine C cycling considering estuarine gradients and dynamics, linkages among estuarine ecosystems, and effects of global change. Through sequencing of samples along the Elbe Estuary we will describe the dominant microorganisms on a phylum and subphylum level for phytoplankton, bacteria, archaea and fungi (e.g., in soil, water, rhizosphere, fecal samples). We will decipher the metabolic pathways actively involved in carbon cycling in water, soils, sediments and estuarine plant rhizospheres. We will see the impact of salinity gradient on the fecal microbiota of small mammals and the gill microbiome of fish. We will also describe the broad transcriptomic stress status of the main fish species in the Elbe estuary.

河口覆盖了全球陆地表面的五分之一以上，是全球碳固定的关键驱动力。它们的特点是由于外部强迫（例如淡水排放、洋流、潮汐能）的变化而具有高度的时间动态，盐度、水动力学和氧气可用性（河口梯度）。沿着河口梯度，潮间带生态系统（有植被的潮沼、有微型底栖植物群落的潮间带）和潮下水道生态系统（底栖和远洋生态系统）紧密相连，产生、运输和处理大量的陆地和水生有机物。控制河口C循环的关键过程仍然知之甚少，虽然最近的研究已经举例说明了潜在的高相关性的植物和phytophyteton-microbial相互作用和草食动物作为控制C通量在潮汐沼泽和河口通道。该建议连同RTG 2530旨在系统地分析生物介导的河口C循环的影响，考虑河口梯度和动态，河口生态系统之间的联系，以及全球变化的影响。通过对沿着易北河口的样品进行测序，我们将在浮游植物、细菌、古生菌和真菌的门和亚门水平上描述优势微生物（例如，在土壤、水、根际、粪便样品中）。我们将破译积极参与水，土壤，沉积物和河口植物根际碳循环的代谢途径。我们将看到盐度梯度对小型哺乳动物粪便微生物和鱼类鳃微生物的影响。我们还将描述易北河河口的主要鱼类的广泛的转录应激状态。