線虫における減数分裂機構の解析

线虫减数分裂机制分析

• 批准号: 09264223
• 负责人: 渡邉 信元
• 金额: $ 1.15万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09264223/
• 关键词: 細胞周期; 減数分裂; 分子生物学; 線虫; チェックポイント

〈Weel様タンパク質の強制発現の減数分裂に対する影響の解析〉平成8年度の研究で単離された2種のWeel様タンパク質(あるいはそのキナーゼ活性変異タンパク質)を,それぞれGFPとの融合タンパク質として,線虫内で強制発現させた.発現させた個体からは対照と同様に子孫が得られ,Weel様タンパク質の強制発現のみでは減数分裂に顕著な影響を与えないことが明らかになった.しかし,融合タンパク質の発現をGFPの発光により解析したところ生殖細胞での発現が必ずしも明らかでなく,用いるプロモーターシステムなどの検討が必要であることが明らかになった.〈線虫サイクリン・Cdc2タンパク質の大量調製〉平成8年度の研究で,2種の線虫Weel様タンパク質が,ヒトCdc2のT14,Y15の両者をリン酸化するが,T14,Y15に対して逆の基質親和性を有するという興味深い結果が得られている.この結果が線虫Cdc2(他の生物のCdc2に比べN末が長いなどの特徴を持つ)に対しても同様であるか否かを検討するために,線虫サイクリン・Cdc2タンパク質の大量調製を試みた.しかし,野性型Cdc2,リン酸化される部位に変異を入れたCdc2のバキュロウイルスの系での発現は確認されたが,その可溶化に現在までのところ成功しておらず,可溶化の条件を検討中である.〈Weelファミリー遺伝子欠損株の解析〉Tc1トランスポゾンの挿入によりWeel様遺伝子の欠損が起こっている線虫変異株を単離を試みた.しかし,現在までのところ欠損が明らかな株の単離には至っていない.これは,Weel様遺伝子のそれぞれが個体の維持に重要な役割を有するためと考えられた.現在,Weel様遺伝子のアンチセンス遺伝子を導入し特定の条件下で発現させることでこの可能性を確認している。

"Analysis of the effects of meiosis on stress development of Weel elements." In the study of Heisei 8, two kinds of Weel elements were isolated, including GFP and fusion elements, and stress development in line. In the process of development, the individual is divided into two groups: the first group is divided into two groups: the second group is divided into two groups: the first group is divided into three groups: the first group is divided into two groups: the second group is divided into three groups: the first group is divided into two groups: the first group is divided into three groups: the second group is divided into three groups: the first group is divided into three groups: the first group is divided into three groups: the second group is divided into three groups: the first group is divided into three groups: the first group is divided into three groups: the second group is divided into three groups: the first group is divided into three groups: the second group is divided into three groups: the first group is divided into three groups: the second group is divided into three groups: the first group is divided into three groups: the second group is divided into three groups: the third group is divided into three groups: the first group is divided into three groups: the first group is divided into three groups: the second group is divided into three groups: the first group is divided into three groups: the first group is divided into three groups: the first The fusion protein is expressed in GFP, and the analysis of GFP is necessary for the development of germ cells. In the study conducted in 2008, two species of nematode weel species were studied, and the results showed that there was a strong correlation between the affinity of T14 and Y15 and the substrate affinity of Cdc2 and Cdc2. The results are as follows: Cdc 2 (Cdc 2 of other organisms is longer than Cdc 2), Cdc 2 is longer than Cdc 2, Cdc 2 is longer than Cdc 2. The discovery of Cdc-2 in the presence of different sites of Cdc-2, Cdc-2 and Cdc-2 in the presence of different sites of Cdc-2, Cdc-2 and Cdc-2 was confirmed. <Analysis of Weel's Loss of Subspecies> Tc 1, T 2, T 3, T 4, T 5, T 6, T 7, T 8, T 8, T 9, T And now we're going to lose some of our power. This is a very important part of the individual's maintenance. Now, the Weel vector is introduced under certain conditions and the possibility of its occurrence is confirmed.

项目成果

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Shuichi Kotani：“PKA 和 MPF 激活的 Polo 样激酶调节后期促进复合物活性和有丝分裂进展。”

Veronigue Baldin: "Evidence for a mammalian Niml-like kinase pathway acting at the GO-1/s transition." Biochem.Biophys,Res.Comm. 236. 130-134 (1997)

Veronigue Baldin：“哺乳动物 Niml 样激酶途径作用于 GO-1/s 转变的证据。”