Research on development and sex differentiation of mouse fetal germ cells
小鼠胎儿生殖细胞发育及性别分化的研究
基本信息
- 批准号:11234201
- 负责人:
- 金额:$ 97.92万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research on Priority Areas
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Mouse primordial germ cells (PGCs) migrate from the base of allantois to the genital ridge. They proliferate during the migration and after the arrival until initiation of the sex-differentiation of fetal gonads. Then, PGCS enter into the prophase of the first meiotic division in the ovary to become oocytes, while those in the testis become mitotically arrested to become prospermatogonia. Relatively little was known : about differentiation of PGCs into oocytes or prospermatogonia in fetal gonads. Observation of ectopic germ cells and studies of cultured PGCs have indicated that both male and female PGCs show cell-autonomous entry into meiosis and differentiation into oocytes if they were not in the fetal testis.We showed that both female and male PGCs isolated from the fetal gonads immediately after their arrival, or those isolated from the mesenteries at 9.5-10.5 dpc during their migration, entered into the leptotene stage of the first meiotic division after dissociation and cultivati … More on for a few days on feeder cells, thus showing that meiotic competence is already acquired by PGCs before reaching the fetal gonads. We also demonstrated that the LIF/gp130-mediated signal suppressed the meiotic transition by PGCs in vitro.Apoptosis is another remarkable event in the fetal germ cells. We investigated regulation of the germ cell apoptosis by using a mouse strain in which bcl-x gene was disrupted. Analysis of heterozygotic embryos and mice revealed that prospermatogonia are more prone to apoptosis than oocytes. Such differences appeared at the beginning of sex-differentiation of germ cells.We also investigated the mouse tudor-related gene, mtr-1, in mouse male germ cells. It is expressed in the prospermatogonia in fetal testis and more abundantly in spermatocytes in adult testes. In mice, nuage structures called chromatoid bodies appear during postnatal spermatogenesis rather than during germ-line specification. Previously, a mouse homologue of vasa, one of the Drosophila polar granule components, has been identified. Its product was present in chromatoid bodies, and the targeted disruption of the gene showed defects in postnatal spermatogenesis. TUDOR is another component of polar granules in Drosophila, and it contains ten repeated copies of the tudor domain. We have identified a novel mtr-1 gene that encodes four repeated copies of the tudor domain. Mtr-1 expression was restricted to germ-line cells, and the transcript was most abundant in spermatocytes in the adult testis. The MTR-1 protein was present predominantly in spermatocytes and round spermatids, showing granular distribution in the cytoplasm. These granules co-localized precisely with those of cytoplasmic ribonucleoproteins, and they were present exclusively in both the inter-mitochondrial nuages and perinuclear chromatoid bodies. Less
小鼠原始生殖细胞(PGCs)从尿囊底部迁移到生殖器嵴。它们在迁移过程中和到达后增殖,直到胎儿性腺的性别分化开始。然后,PGCS在卵巢中进入第一次减数分裂的前期,成为卵母细胞,而在睾丸中的PGCS则被有丝分裂阻滞,成为精原细胞。关于PGCs在胎儿性腺中分化为卵母细胞或卵母细胞的情况,目前知之甚少。对异位生殖细胞的观察和体外培养的PGCs的研究表明,无论是雄性还是雌性PGCs,如果它们不在胎儿睾丸内,都能自主地进入减数分裂并分化为卵母细胞。我们发现,无论是从胎儿性腺中分离的,还是在9.5-10.5 dpc时从肠系膜中分离的,经分离培养后进入第一次减数分裂的细线期, ...更多信息 在饲养细胞上持续几天,从而表明PGCs在到达胎儿性腺之前已经获得了减数分裂能力。我们还证实了LIF/gp 130介导的信号在体外抑制PGCs的减数分裂转变。我们使用bcl-x基因被破坏的小鼠品系来研究生殖细胞凋亡的调控。对杂合子胚胎和小鼠的分析表明,卵母细胞比卵母细胞更容易凋亡。我们还研究了小鼠雄性生殖细胞中的mtr-1基因。它在胎儿睾丸的精原细胞中表达,在成年睾丸的精母细胞中表达更丰富。在小鼠中,被称为拟染色体的核结构出现在出生后的精子发生过程中,而不是在生殖细胞特化过程中。此前,已经确定了果蝇极粒成分之一vasa的小鼠同源物。其产物存在于拟染色体中,并且该基因的靶向破坏显示出出生后精子发生的缺陷。TUDOR是果蝇极性颗粒的另一个组成部分,它包含十个重复的tudor结构域拷贝。我们已经确定了一个新的mtr-1基因,编码四个重复拷贝的都铎结构域。Mtr-1的表达仅限于生殖系细胞,并且在成年睾丸的精母细胞中表达最丰富。MTR-1蛋白主要存在于精母细胞和圆形精子细胞中,在细胞质中呈颗粒状分布。这些颗粒共定位精确的细胞质核糖核蛋白,它们是唯一存在于线粒体间的nuages和核周类染色体体。少
项目成果
期刊论文数量(63)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kanno, Y., Tamura, M., Chuma, S., Sakurai, T., Machida, T., Nakatsuji, N.: "A cystatin-related gene, testatin/cresp, shows male-specific expression in germ and somatic cells from the initial stage of murine gonadal sex-differentiation"Int. J. Dev. Biol..
Kanno, Y.、Tamura, M.、Chuma, S.、Sakurai, T.、Machida, T.、Nakatsuji, N.:“半胱氨酸蛋白酶抑制剂相关基因 testatin/cresp 在生殖细胞和体细胞中显示出雄性特异性表达
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- 影响因子:0
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Chuma, S., Nakatsuji, N.: "Autonomous transition into meiosis of mouse fetal germ cells in vitro and its inhibition by gp130-mediated signaling"Devel. Biol.. 229. 468-479 (2001)
Chuma, S., Nakatsuji, N.:“体外小鼠胎儿生殖细胞自主转变为减数分裂及其受 gp130 介导的信号传导的抑制”Devel。
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中辻憲夫(編): "実験医学別冊 ポストゲノム時代の実験講座4 幹細胞・クローン研究プロトコール"羊土社. 254 (2001)
Norio Nakatsuji(编辑):“实验医学特刊:后基因组时代 4 干细胞/克隆研究方案的实验课程”Yodosha 254 (2001)。
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Nakatsuji, N., Chuma, S.: "Differentiation of mouse primordial germ cells into female or male germ cells"Int. J. Dev. Biol.. 45. 541-548 (2001)
Nakatsuji,N.,Chuma,S.:“小鼠原始生殖细胞分化为雌性或雄性生殖细胞”Int。
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T. Noce, S. Okamoto, and N. Tsunekawa: "Vasa homolog genes in mammalian germ cell development"Cell Struc. & Func.. 26. 131-136 (2001)
T. Noce、S. Okamoto 和 N. Tsunekawa:“哺乳动物生殖细胞发育中的 Vasa 同源基因”Cell Struc。
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NAKATSUJI Norio其他文献
NAKATSUJI Norio的其他文献
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{{ truncateString('NAKATSUJI Norio', 18)}}的其他基金
Establishment of a isolation and maturation system for human adult hepatic stem cells
人成体肝干细胞分离成熟体系的建立
- 批准号:
14370384 - 财政年份:2002
- 资助金额:
$ 97.92万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Regulative Mechanisms and Manipulation of the Germ Cell Lineage
生殖细胞谱系的调节机制和操纵
- 批准号:
11234101 - 财政年份:1999
- 资助金额:
$ 97.92万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Research on Development and Sex-Differentiation of Mouse Germ Cells
小鼠生殖细胞发育及性别分化的研究
- 批准号:
11480223 - 财政年份:1999
- 资助金额:
$ 97.92万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Research on regulation mechanisms of development and differentiation of mouse primordial germ cells
小鼠原始生殖细胞发育分化调控机制研究
- 批准号:
08458241 - 财政年份:1996
- 资助金额:
$ 97.92万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Research on manipulation of mammalian fetal germ cells and gene transfection
哺乳动物胎儿生殖细胞操作及基因转染研究
- 批准号:
07558290 - 财政年份:1995
- 资助金额:
$ 97.92万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Research on control mechanisms of growth and differentiation of mouse primordial germ cells and their developmental manipulation
小鼠原始生殖细胞生长分化调控机制及其发育调控研究
- 批准号:
05454655 - 财政年份:1993
- 资助金额:
$ 97.92万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)