Regulative Mechanisms and Manipulation of the Germ Cell Lineage

生殖细胞谱系的调节机制和操纵

• 批准号: 11234101
• 负责人: NAKATSUJI Norio
• 金额: $ 9.02万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11234101/
• 关键词: Germ cells; mammalian; embryo manipulation; germ lineage

This research group was aimed at facilitating and encouraging scientific interactions and collaboration among the scientists involved in this priority area. For such purpose, this group organized annual meeting of the priority area, and also symposia open to general scientists. The most significant was the international symposium on "Development and Epigenetics of Mammalian Germ Cells and Pluripotent Stem Cells, "held on 19-21 November, 2001 in Kyoto, with more than 300 participants and 25 speakers.Speakers includes : Anne McLaren (Cambridge, UK) "Germ cells and stem cells : the key to developmental potency"/Patrick Tam (Sydney, Australia) "Allocation and differentiation of the progenitors for germ cells and urogenital tissues"/Austin Smith (Edinburgh, UK) "Signalling self-renewal in pluripotent stem cells"/Guang-Quan Zhao (Dallas, USA) "BMP signaling in the generation of primordial germ cells in the mouse"/Chris Wylie (Cincinnati, USA)"Migration of mouse primordial germ cells"/Robin L … More ovell-Badge (London, UK)"The Molecular Genetics of Sex Determination in Mammals : Building Pathways"/Azim Surani (Cambridge, UK)"The origin and properties of the mouse germ line."/N. B. Hecht (Philadelphia, USA)"Selective intracellular and intercellular mRNA transport in mammalian germ cells"/Hitoshi Niwa (Center for Developmental Biology, RIKEN, Japan) /Yukio Tsunoda (Kinki Univ., Japan)/Nobuo Takagi (Hokkaido Univ., Japan)/Hiroyuki Sasaki (Nat. Inst. of Genetics, Japan)/Fumitoshi Ishino (Tokyo Inst. for Technology, Japan)"Genomic imprinting in the germ cell lineage"/Takashi Tada (Kyoto Univ., Japan)/Junji Takeda (Osaka Univ., Japan)/Kuniya Abe (Kumamoto Univ., Japan), Yasuhisa Matsui (Osaka Medical Center for Maternal and Child Health, Japan), Toshiaki Noce (Mitsubishi-Kagaku Inst. of Life Sciences, Japan), Norio Nakatsuji (Kyoto Univ., Japan), Tomohiro Kono (Tokyo Univ. of Agriculture, Japan), Atsuo Ogura (Nat. Inst. of Infectious Diseases, Japan), Hiroshi Nojima (Osaka Univ., Japan), Masami Nozaki (Osaka Univ., Japan), Masaru Okabe (Osaka Univ., Japan), Gen Kondoh (Osaka Univ., Japan) Less

该研究小组旨在促进和鼓励参与这一优先领域的科学家之间的科学互动和合作。为此，该小组组织了重点领域的年度会议，并向普通科学家开放专题讨论会。其中最重要的是2001年11月19日至21日在京都举行的“哺乳动物生殖细胞和多能干细胞的发育和表观遗传学”国际研讨会，有300多名与会者和25名发言人。演讲嘉宾包括：Anne McLaren（剑桥，英国）“生殖细胞和干细胞：发育潜能的关键”/Patrick Tam（悉尼，澳大利亚）“生殖细胞和泌尿生殖组织的前体分配和分化”/Austin Smith（爱丁堡，英国）“多能干细胞中的自我更新信号”/赵广全（达拉斯，美国）“BMP信号在小鼠原始生殖细胞的产生”/Chris Wylie（辛辛那提，美国）“小鼠原始生殖细胞的迁移”/Robin L…More ovell-Badge(伦敦，英国)《哺乳动物性别决定的分子遗传学：构建途径》/Azim Surani （Cambridge， UK）《小鼠生殖系的起源和特性》/N。B. Hecht（美国费城）“哺乳动物生殖细胞中细胞内和细胞间mRNA的选择性转运”/ Niwa仁（日本理化研究所发育生物学中心）/ Yukio Tsunoda（日本近畿大学）/Nobuo Takagi（日本北海道大学）/Hiroyuki Sasaki（日本国立遗传学研究所）/Fumitoshi Ishino（日本东京技术研究所）“生殖细胞谱系中的基因组印记”/Takashi Tada（日本京都大学）/Junji Takeda（日本大阪大学）/Kuniya Abe（日本熊本大学），松井康久（日本大阪妇幼保健医疗中心），野司敏明（日本三菱鹿洲生命科学研究所），中津治则夫（日本京都大学），河野友广（日本东京农业大学），小仓笃男（日本国立传染病研究所），野岛浩（日本大阪大学），野崎雅美（日本大阪大学），冈部雅鲁（日本大阪大学），近藤元(日本大阪大学

项目成果

Kono T, Sotomaru Y, L Dandolo.: "Mouse parthenogenetic embryos with monoallelic H19 expression can develop to day 17.5 of gestation."Dev.Biol.. 243. 294-300 (2002)

Kono T、Sotomaru Y、L Dandolo.：“具有单等位基因 H19 表达的小鼠孤雌胚胎可以发育至妊娠第 17.5 天。”Dev.Biol.. 243. 294-300 (2002)

Nakatsuji, N., Chuma, S.: "Differentiation of mouse primordial germ cells into female or male germ cells"Int. J. Dev. Biol.. 45. 541-548 (2001)

Nakatsuji，N.，Chuma，S.：“小鼠原始生殖细胞分化为雌性或雄性生殖细胞”Int。

T. Noce, S. Okamoto, and N. Tsunekawa: "Vasa homolog genes in mammalian germ cell development"Cell Struc. & Func.. 26. 131-136 (2001)

T. Noce、S. Okamoto 和 N. Tsunekawa：“哺乳动物生殖细胞发育中的 Vasa 同源基因”Cell Struc。

Hasegawa, K., Nakatsuji, N.: "Insulators prevent transcriptional interference between two promoters in a double gene construct for transgenesis"FEBS Lett.. 520. 47-52 (2002)

Hasekawa, K., Nakatsuji, N.：“绝缘子防止转基因双基因构建体中两个启动子之间的转录干扰”FEBS Lett.. 520. 47-52 (2002)

Tamura, M., Kanno, Y., Chuma, S., Saito, T., Nakatsuji, N.: "Pod-1/Capsulin shows a sex-and stage-dependent expression pattern in the mouse gonad development and represses expression of Ad4BP/SF-1"Mech. Devel.. 102. 135-144 (2001)

Tamura, M.、Kanno, Y.、Chuma, S.、Saito, T.、Nakatsuji, N.：“Pod-1/Capsulin 在小鼠性腺发育中显示出性别和阶段依赖性表达模式，并抑制