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ヘリコバクター・ピロリ外膜蛋白の構造多様性と宿主免疫回避機構

幽门螺杆菌外膜蛋白的结构多样性与宿主免疫逃避机制

基本信息

批准号：
14021102
负责人：
西園晃
金额：
$ 4.35万
依托单位：
大分医科大学
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2002
资助国家：
日本
起止时间：
2002 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14021102/
关键词：
遺伝子感染症細菌微生物ヘリコバクター・ピロリ

项目摘要

今年度はH.pyloriの外膜蛋白Omp29の直系遺伝子群の構造の多様性と宿主免疫系からの回避機構との関係についての検討と、初感染時におけるH.pyloriに対する免疫応答がどのように成立機構について検討した。1.新規外膜蛋白Omp29に関する検討大腸菌組み換え蛋白として発現させたOmp29に対する血中抗体は、乳幼児血清を用いた検討では0歳児で既に4/23(17%)が抗体を保有し、1歳前後から早くも抗体の陽転現象が見られ、非感染成人では6/19(31%)が陽性であった。つまり本抗原は感染曝露を受けるにつれ陽性率が上昇するが、健常人にも抗体を誘導するような菌体抗原であり、病原性との関連は薄いことが予想された。2.H.pylori感染成立初期応答におけるCTLA-4分子の役割非経口的な菌体抗原の免疫後による実験的H.pylori感染成立初期の免疫応答の検討を行った。感染初期から18週までの観察で感染マウス胃ではリンパ球を主体とする炎症細胞浸潤を強く認めた。一方免疫後感染させた群では、H.pyloriの生着菌量は感染群と差はないものの炎症細胞浸潤はほとんど認められなかった感染マウス群はTh1サイトカインが優位の炎症だが、免疫群は事前の免疫により特異抗原とマイトゲンに対してのIL-2、IFN-γの産生抑制が著しく、T細胞の低反応状態が示唆された。またT細胞表面のCTLA-4の発現は、感染72時間後には、免疫群において有意な発現の増多を示していた。また抗CTLA-4抗体をin vivoで投与することで、血中IgGlの増加が早期に強力に誘導され、IL-4の増加がIFN-γに比べより優位に認められた。このことは感染初期のTh2バランスへのシフトがその後の炎症の沈静化を誘導し、補助分子CTLA-4活性化を介したT細胞応答の抑制が結果的にH.pylori胃炎を抑制したと予想された。

This year, the direct subgroup of H.pylori outer membrane protein Omp29 has established a multipotent host immune system, which has been established by the avoidance mechanism of multiple host immune system, the immune response of H.pylori antibody at the time of first infection and the establishment of the established organ. 1. The new outer membrane protein (Omp29) was detected in E. coli. The antibody in the blood was detected in the Omp29 group, the antibody in the serum of the breast and baby was found to be 23% (17%), the antibody was retained in 23% (17%), the antibody in the non-infected adult was 619% (31%), and the antibody was retained in the serum of breast-feeding infants. The infection exposure of this antigen is affected by the sex rate of the virus, the antibody of the normal person leads to the infection of the bacterial antigen, and the pathogenicity of the virus is thin. In the early stage of 2.H.pylori infection, the CTLA-4 molecule was used to immunize the non-oral "somatic antigen" and then immunized with the H.pylori infection in the early stage of the establishment. In the early stage of the infection, the infection was observed in the stomach, the main body of the ball, the inflammation, the cell infiltration and the strong infection. After one party was immunized, the number of bacteria infected, the number of bacteria infected, the number of bacteria, the amount of bacteria, the number of bacteria, the number of bacteria, the amount of bacteria, the amount of bacteria, the number of bacteria, the amount of bacteria, the amount of bacteria, the number of bacteria, the number of bacteria, the amount of bacteria, the number of bacteria, the number of bacteria, the amount of bacteria, the number of bacteria, the number of bacteria, the amount of bacteria, the number of bacteria, the number The T cell has a low level of reflex, indicating that it is abetted. The presence of CTLA-4 on the cell surface of T cells, 72 hours after infection, and the intentional presence of multiple antibodies in the immune group indicated that there were multiple signs of infection. The anti-CTLA-4 antibody "in vivo" was injected into the serum, IgGl was added to the blood, and IL-4 was added to the IFN- γ antibody. In the early stage of infection, Th2 was detected. After infection, inflammation was induced by sedation and activation of CTLA-4. The activation of H.pylori was mediated by T cells. The results of inhibition showed that H.pylori gastritis was suppressed.