Functions of RecQ family helicases in DNA replication, repair, and damage avoidance

RecQ 家族解旋酶在 DNA 复制、修复和避免损伤中的功能

• 批准号: 17013005
• 负责人: ENOMOTO Takemi
• 金额: $ 45.76万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17013005/
• 关键词: 癌; ブルーム症候群; ロスモンド・トムソン症候群; RecQファミリーへリカーゼ; DNA複製; DNA組換え; 姉妹染色分体接着; RecQファミリーヘレカーゼ; DNA傷害回避; BLM; RECQL4; KNA組換え; ブルーム症侯群; RecQフミリーヘリカーゼ; RECL4; RecQヘリカーゼ; Sgs1; DNA複製フォーク; 傷害回避; ワーナー症候群; Sgsl; 障害回避

Human cells possess five RecQ family helicases, RECQL1, BLM, WRN, RTS, and RECQL5, whose defects cause a predisposition to cancer. In this study, functions of these RecQ family helicases were investigated and following results were obtained. BLM functions at the last stage of DNA replication, segregation of replicated DNA and sister chromatid cohesion. RTS is involved in DNA replication and repair of DNA double strand breaks. Finally, RECQL1 and RECQL5 are involved in early stages of recombination.

人类细胞具有五个RECQ家族解旋酶，RecQL1，BLM，WRN，RTS和RECQL5，它们的缺陷会导致癌症的易感性。在这项研究中，研究了这些RECQ家族解旋酶的功能，并获得了以下结果。 BLM在DNA复制的最后阶段起作用，复制DNA的分离和姐妹染色单体凝聚力。 RTS参与DNA复制和DNA双链断裂的修复。最后，RECQL1和RECQL5参与重组的早期阶段。

项目成果

RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability

• DOI: 10.1101/gad.1708608
• 发表时间: 2008-10-15
• 期刊: GENES & DEVELOPMENT
• 影响因子: 10.5
• 作者: Xu, Dongyi;Guo, Rong;Wang, Weidong
• 通讯作者: Wang, Weidong

RecQL5 promotes genome stabilization through two parallel mechanism Interacting with RNA polymerase II and acting as a helicase

RecQL5 通过两个并行机制促进基因组稳定：与 RNA 聚合酶 II 相互作用并充当解旋酶

• 发表时间: 2010
• 期刊: Mol.Cell.Biol. (in press)
• 作者: Islam;M. N.
• 通讯作者: M. N.

Mgsl and Rad18/Rad5/Mms2 are require for survival of novel temperature/cold sensitive mutant alleles of Pol31, the second subunit of DNA polymerase d, in Sacharomyces cerevisiae.

Mgsl和Rad18/Rad5/Mms2是酿酒酵母中DNA聚合酶d的第二个亚基Pol31的新型温度/冷敏感突变等位基因的生存所必需的。

• 发表时间: 2006
• 期刊: DNA Repair 5・12
• 作者: Akagi T;Ito T;Kato M;Jin Z;Cheng Y;Kan T;Yamamoto G;Olaru A;Kawamata N;Boult J;Soukiasian HJ;Miller CW;Ogawa S;Meltzer SJ;Koeffler HP.;N.Davoodi Vijeh Motlagh
• 通讯作者: N.Davoodi Vijeh Motlagh

Repression of nascent strand elongation by deregulated Cdtl during DNA replication in Xenonus egg extracts

蟾蜍卵提取物中 DNA 复制过程中 Cdtl 失调对新生链伸长的抑制

• 发表时间: 2009
• 期刊: Mol. Biol. Cell 20
• 作者: Tsuyama;T
• 通讯作者: T

Global analysis of mutual interaction surfaces of nucleosomes with comprehensive point mutants

• DOI: 10.1111/j.1365-2443.2009.01350.x
• 发表时间: 2009-11
• 期刊: Genes to Cells
• 影响因子: 2.1
• 作者: M. Sakamoto;Shuhei Noguchi;S. Kawashima;Yusuke Okada;T. Enomoto;M. Seki;M. Horikoshi