Functions of RECQL1 and RECQL5 in the maintenance of genome stability

在本研究中，我主要研究了RECQL5的功能。对RECQL5基因敲除（KO）细胞的分析显示，RECQL5参与DNA链间交联（ICL）修复。本研究结果表明，在Rad51-ssDNA丝形成后，RECQL5在BRCA2下游发挥作用，可能会破坏废弃的丝。此外，cddp诱导的免疫球蛋白位点基因转化的频率和变异在RECQL5 koccells中增加。这些结果表明，RECQL5在调节icl诱导的重组发生率和质量方面发挥了作用。

In this study, I mainly focused on the function of RECQL5. Analyses using RECQL5 gene knockout (KO) cells revealed that RECQL5 is involved in DNA interstrand crosslink (ICL) repair. The results obtained in this study suggest that RECQL5 functions down stream of BRCA2, after formation of Rad51-ssDNA filament probably disrupting disused filaments. Furthermore, the frequency and variation of CDDP-induced gene conversion at the immunoglobulin locus were increased in RECQL5 KOcells. These results suggest that RECQL5 plays a role in regulating the incidence and quality of ICL-induced recombination.